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Identification of Macro-Algae Species in Sindangkerta Beach, Tasikmalaya District, an Effort to Explore the Biodiversity of Indigenous Species Sri Handayani; Cipto Utomo; Nuraidah Nuraidah; Indrastiwi Pramuliati; Achmad Fahreza
Journal of Tropical Biodiversity Vol 1 No 1 (2020): December 2020
Publisher : Universitas Nasional Jakarta

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Abstract

Sindangkerta beach has a total area of ​​90 ha, located in the Sindangkerta village, which is one of the natural tourism areas located on the south coast of West Java, which became one of the mainstays of Tasikmalaya regency. The tourist area is relatively unspoiled and has not maximized its potential. Aside from being a tourist area, Sindangkerta Beach is also a conservation area for sea-turtle breeding. As one of the conservation areas and a tourist spot is certainly a lot of pressure and ecological threats. Handling indigenous species diversity is very important and urgent treated as early as possible to monitor the occurrence of extinction and efforts to develop the existing potential. Data species and morphological descriptions for basic data management and enrichment of biodiversity, especially in Sindangkerta Beach Tasikmalaya. This study was conducted in June 2013 at the Sindangkerta Coastal Waters, District Cipatujah, Tasikmalaya District, West Java Province. The method used Line Transect Square. Sampling was carried out during low tide using square plot. All types of macroalgae found in the square plot were calculated for the number of individuals before the identification process. Macroalgae found in Sindangkerta Beach were 25 species and 17 genera of 3 divisions namely Chlorophyta, Phaeophyta, and Rhodophyta. The highest abundance of macroalgae is Padina australis and Sargassum duplicatum, while the lowest abundance is Halimeda discoidea, Halimeda macroloba, and Laurencia sp. Types of macroalgae that dominate a group Sindangkerta Beach Aquatic included as Phaeophyta (brown algae) categories with the highest dominance type of Padina autralis.
Phytochemical, Antibacterial and Antioxidant Activities Test of Three Macro-Algae Phaeophyceae Extracts from Pulau Tidung Coastal Kepulauan Seribu Sri Handayani; Suprihatin Suprihatin; Retno Widowati; Nyoman Yudi; Indra Rahayu
Journal of Tropical Biodiversity Vol 1 No 1 (2020): December 2020
Publisher : Universitas Nasional Jakarta

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Abstract

Macro-algae is a Thallophyta plant that has been known to have potential as a food and medicine ingredient, one of that is in the Kepulauan Seribu, particularly on Pulau Tidung coastal. Therefore this study aimed to explore the potential of three types of macro-algae: Hormophysa triquerta, Padina austraslis, and Sargassum binderi (Phaeophyceae) on the Pu;au Tidung coastal by revealing the phytochemical content, antibacterial, and antioxidant activity. The design used in this study was completelyrandomized factorial designs. The phytochemicals tested were flavonoid, alkaloid, tannin, saponin, quinon, steroid, and terpenoid. Antibacterial were tested by diffusion wel method on Muller Hinton agar in a petri dishes with a size of 100 mm and a thickness of 10 mm. The three tested macro-algae were extracted using methanol solvent and divided into four concentrations: 5%, 10%, 15%, and 20%. The test bacteria used were Staphylococcus aureus ATCC 6539 and Escherichia coli ATCC 8739. Observation of the antibacterial test was carried out 24 hours after incubation at 370C. Antioxidant activity at concentrations of 100, 200, 300, 500, 700 ppm was carried out by the DPPH (1,1-diphenil-2-pikrylhidrazyl) method. The results of phytochemical tests showed that flavonoids and steroids were found in the three macro-algae tested, while saponins were only found in S. binderi. The results of the inhibition zone showed that the three macroalgae extract tested did not inhibit E. coli bacteria. The largest inhibition zone was shown at a concentration of 20% against S. aureus bacteria, namely, H. triquerta = 10.09 ± 0.89, P. austraslis = 11, 46 ± 1.09, and S. binderi 8.00 ± 1.29. The regression results of the tested macro -algae antioxidant activity showed that the IC50 values were 13,290 ppm (H. triquerta), 3,800 ppm (P. australis), and 4,053 ppm (S. binderi).