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All Journal Makara Journal of Technology Bisma : Business and Management Journal
Hertanto, Robby
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Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Electrical & Electronics Engineering Engineering Materials Science & Nanotechnology Mechanical Engineering Social Sciences

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Expression and the Functional Study of Fusion Proteins α-Amylase and Hemolysin- αas an Application in Biofilm Polysaccharide Degradation Sugiarta, Gede Yuda; Wiseso, Anggoro; Sari, Siska Yuliana; Kamila, Etri Dian; Geraldine, Vanessa; Christina, Diana; Hanifi, Muhammad; Satyapertiwi, Dwiantari; Hertanto, Robby; Bela, Budiman; Yohda, Masafumi; Sahlan, Muhamad
Makara Journal of Technology Vol. 20, No. 3
Publisher : UI Scholars Hub

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Abstract

Biofilm is an aggregate of consortium bacteria that adhere to each other on a surface. It is usually protected by the exopolysaccharide layer. Various invasive medical procedures, such as catheterization, endotracheal tube installation, and contact lens utilization, are vulnerable to biofilm infection. The National Institute of Health (NIH) estimates 65% of all microbial infections are caused by biofilm. Periplasmic α-amylase (MalS) is an enzyme that hydrolyzes α-1, 4- glicosidic bond in glycogen, starch, and others related polysaccharides in periplasmic space. Another protein called hemolysin-α (HlyA) is a secretion signal protein on C terminal of particular peptide in gram negative bacteria. We proposed a novel recombinant plasmid expressing α-amylase and hemolysin-α fusion in pSB1C3 which is cloned into E.coli to enable α-amylase excretion to extracellular for degrading biofilm polysaccharides content, as in starch agar. Microtiter assay was performed to analyze the reduction percentage of biofilm by adding recombinant E.coli into media. This system is more effective in degrading biofilm from gram positive bacteria i.e.: Bacillus substilis (30.21%) and Staphylococcus aureus (24.20%), and less effective degrading biofilm of gram negative i.e.: Vibrio cholera (5.30%), Pseudomonas aeruginosa (8.50%), Klebsiella pneumonia (6.75%) and E. coli (-0.6%). Gram positive bacteria have a thick layer of peptidoglycan, causing the enzyme to work more effectively in degrading polysaccharides.
Pengaruh WLB dan Keadilan Organisasi terhadap Loyalitas Karyawan Generasi Z melalui Kepercayaan Organisasi Rahmawati, Yunita Devi; Prasetyo, Hendy Budi; Arsyad, Nurul Arsyianti; Effendy, Saimon; Yusnita, Irma; Wijaya, Bayu Arya; Girawan, Agi Herdyandyah; Kusumawati, Vita Maya; Hertanto, Robby; Prajogi, Liena; Gunawan, Andreas W
BISMA : Business and Management Journal Vol. 3 No. 4 (2025): Bisma : Business and Management Journal
Publisher : CV. Kalimasada Group

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59966/bisma.v3i4.2403

Abstract

This study examines the effects of work–life balance and organizational justice on Generation Z employees’ loyalty, with organizational trust as a mediating variable, within the context of industrial relations at a private security services company in Jakarta. A quantitative approach was employed using an online survey distributed to Generation Z employees. Data were analyzed using Partial Least Squares–Structural Equation Modeling (PLS-SEM). The results indicate that work–life balance does not have a significant effect on organizational trust or employee loyalty. In contrast, organizational justice has a positive and significant effect on both organizational trust and employee loyalty. Furthermore, organizational trust does not mediate the relationship between work–life balance and employee loyalty; however, it significantly strengthens the relationship between organizational justice and employee loyalty. These findings suggest that, in a high-pressure and non-conventional work context, perceptions of fairness in procedures, interactions, and resource distribution play a more decisive role in shaping trust and loyalty among Generation Z employees than work–life balance initiatives alone. The study contributes to the organizational behavior literature by highlighting the central role of justice and trust in fostering employee loyalty among younger workforce cohorts and offers practical implications for managerial strategies in similar industrial settings.
Co-Authors Arsyad, Nurul Arsyianti Budiman Bela Christina, Diana Effendy, Saimon Geraldine, Vanessa Girawan, Agi Herdyandyah Gunawan, Andreas W Hanifi, Muhammad Kamila, Etri Dian Kusumawati, Vita Maya Liena Prajogi Prasetyo, Hendy Budi Rahmawati, Yunita Devi SAHLAN, MUHAMAD Sari, Siska Yuliana Satyapertiwi, Dwiantari Sugiarta, Gede Yuda Wijaya, Bayu Arya Wiseso, Anggoro Yohda, Masafumi Yusnita, Irma