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All Journal Biosaintifika: Journal of Biology & Biology Education Biosaintifika: Journal of Biology & Biology Education
Apichat Upaichit, Apichat
Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University Thailand
Agriculture, Biological Sciences & Forestry

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Proteases from Latex of Euphorbia spp. and Its Application on Milk Clot Formation Fibriana, Fidia; Upaichit, Apichat
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.3951

Abstract

Crude proteases were extracted from Euphorbiaceae family, i.e. E. milii var imperata, E. trigona, and E. maculata. Among those three crude proteases, the activity of protease from E. trigona was the highest (812.50 U/ml), whereas E. milii and E. maculata crude proteases activity were 298.60 U/ml and 95.80 U/ml, respectively. E. maculata protein concentration was the highest among those three crude enzymes (1.206 mg/ml). The optimum pH and temperature of the enzymes were pH 7.0, pH 6.0, pH 6.5 and 60 C, 50 C, and 50 C, respectively. Crude protease from E. milii var imperata, E. trigona, and E. maculata retained proteolytic activity over a wide range of pH (5.09.0) and temperature (up to 65 C) with casein as substrate. All crude proteases showed milk clotting activity ranged from 0.58 U/ml to 1.01 U/ml. Thus, these crude proteases are potential to be applied in dairy industries. However, further study on enzyme purification and characterization are necessary to obtain high purity of proteases before its application.Protease kasar berhasil diekstrak dari tanaman family Euphorbiaceae, yaitu E. milii var imperata, E. trigona, dan E. maculata. Diantara ketiga protease tersebut, aktivitas protease tertinggi diperoleh dari E. trigona (812,50 U/ml), sedangkan aktivitas protease dari E. milii dan E. maculata adalah 298,60 U/ml dan 95,80 U/ml, berturut-turut. Konsentrasi total protein tertinggi terdapat pada protease kasar E. maculata (1,206 mg/ml). pH dan suhu optimum ketiga enzim tersebut adalah pH 7.0, pH 6.0, pH 6.5 dan suhu 60 C, 50 C, and 50 C, berturut-turut. Protease kasar dari E. milii var imperata, E. trigona, dan E. maculata menunjukkan aktivitas proteolitik pada rentang pH 5.09.0 dan rentang suhu sampai 65 C menggunakan kasein sebagai substrat. Semua protease kasar menunjukkan aktivitas penggumpalan susu dengan rentang dari 0,58 U/ml sampai 1,01 U/ml. Berdasarkan hasil yang diperoleh, protease kasar dari ketiga jenis tanaman ini berpotensi untuk diaplikasikan dalam industri olahan susu. Meskipun demikian, studi lanjut mengenai purifikasi dan karakterisasi sangat diperlukan untuk memperoleh protease murni sebelum aplikasi dalam industri makanan, khususnya pada industri olahan susu.
A Story-Based Virtual Laboratory Practicum in an Undergraduate Genetics Course to Improve Concept Understanding and Visual Literacy Fibriana, Fidia; Abidin, Zaenal; Naufal, M Ahganiya; Febriyanto, Hendra; Alma’ruf, Fattah; Nurazizah, Kaylha Salsabilla; Ningrum, Nuraini Septia; Upaichit, Apichat
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 2 (2025): August 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i2.23901

Abstract

Genetic concepts, particularly DNA technology, often lead to misconceptions among students. Integrating a virtual laboratory (VL) into coursework presents an innovative approach to enhancing concept understanding and visual literacy through interactive, story-based simulations that bridge theory and practice. This study aimed to analyze the students' concept understanding and visual literacy improvement by applying a VL to teach DNA Test Simulation (Paternity Test) using a contextual narrative method. The research was conducted in the Science Education undergraduate program with a purposive total sampling of students enrolled in Genetics (First Semester 2024/2025), utilizing an open-source web-based VL platform. Pretest and posttest assessments measured students' concept understanding and visual literacy before and after VL integration. The findings reveal a significant improvement, with concept understanding rising from an average pretest score of 42.6 to posttest 69.9 (N-Gain 0.46) and visual literacy increasing from 53.8 to 71.8 (N-Gain 0.37). Furthermore, 97% of students were satisfied with the VL-based learning method. This research contributes to knowledge and science development by demonstrating that VL can improve concept learning and visual literacy in genetics education. It offers an accessible and scalable solution for teaching complex DNA technology concepts, reducing reliance on costly wet laboratory experiments while maintaining scientific rigor. Beyond academia, the study supports broader educational equity, making advanced molecular biology concepts more available to diverse learners and fostering scientific literacy in society.
Co-Authors Alma’ruf, Fattah Febriyanto, Hendra Fidia Fibriana Naufal, M Ahganiya Ningrum, Nuraini Septia Nurazizah, Kaylha Salsabilla Zaenal Abidin