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PHYLOGENETIC RELATIONSHIPS OF LOCAL DURIAN SPECIES BASED ON MORPHOLOGICAL CHARACTERISTICS AND PCR-RFLP ANALYSIS OF THE RIBOSOMAL INTERNAL TRANSCRIBED SPACER (ITS) DNA Fibriana, Fidia; Hadiyanti, Lutfia Nur
Sainteknol : Jurnal Sains dan Teknologi Vol 14, No 2 (2016): December 2016
Publisher : Unnes Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/sainteknol.v14i2.8990

In this study, twentydurian species obtained from Central Java in situ collection were characterized using the morphological characteristics and the restriction patterns which generatedfrom the region spanning the internal transcribed spacers ITS LEU and ITS 4. Morphological characteristics of durian leaf, stem, tree, and fruit showed a variation for the differentspecies, whereas polymerasechain reaction (PCR) products of ribosomal DNA region showed a low length of variation. The size of the PCR products and the restrictionanalyses with the restriction endonucleases Bsp1431yielded a restriction pattern for eachspecies.

PENERAPAN STRATEGI STORY TELLING DALAM PEMBELAJARAN BIOLOGI MOLEKULER BERBASIS KOMPETENSI DAN KONSERVASI Mustikaningtyas, Dewi; Widianti, Tuti; Fibriana, Fidia
Journal of Biology Education Vol 5 No 1 (2016)
Publisher : Journal of Biology Education

Show Abstract | Download Original | Original Source | Check in Google Scholar

Telah dilakukan penelitian pembelajaran Biologi Molekular berbasis Kompetensi dan Konservasi menggunakan strategi Paired Story telling with picture untuk mendiskripsikan bentuk pembelajaran yang dapat menumbuhkembangkan nilai-nilai konservasi dalam diri mahasiswa serta mengetahui efektivitasnya. Subyek penelitian adalah mahasiswa program studi Pendidikan Biologi berjumlah 42 orang angkatan 2013 yang telah menggunakan kurikulum Unnes 2012. Pembelajaran Biologi molekular berbasis kompetensi dan konservasi didiskripsikan sebagai pembelajaran yang mengintegrasikan materi dengan nilai karakter konservasi menggunakan strategi paired storytelling with pictures dengan terlebih dahulu melakukan analisis Standar Kompetensi (SK) dan Kompetensi Dasar (KD) dan pemetaan materi ajar yang memiliki korelasi dengan tema karakter atau nilai-nilai moral dalam konservasi, yang selanjutnya dirumuskan indikator pencapaian SK, KD dengan menyertakan kualitas nilai-nilai konservasi. Pembelajaran yang dilakukan efektif dalam menumbuhkan nilai-nilai karakter dalam diri mahasiswa dengan indikator: (1) hasil penilaian diri (self assessment) 94,07% mahasiswa setuju dan sangat setuju dengan pernyataan tentang nilai karakter yang ada, (2) kemampuan komunikasi lisan mahasiswa dalam storytelling with picture70% mahasiswa memiliki performance sangat baik dan baik, dan (3) tanggapan mahasiswa terhadap pelaksanaan pembelajaran 97% mahasiswa berpendapat bahwa strategi pairedstory telling with pictures melatih kemampuan mahasiswa mengamati dan menganalisis gambar dan menceritakan kembali kepada teman secara tertulis maupun lisan. Molecular Biology and conservation competency-based described as learning that integrates the material with the character of the conservation value using paired strategy storytelling with pictures by analyzing the standard of competence and basic competence. Subjects in this study were 42 students of Biology Education year 2013 who have used the 2012 Unnes curriculum learning. Further, mapping of teaching materials that have a correlation with the theme of character or moral values in conservation was performed. Then, it indicators was formulated for achievement of standard of competence and basic competence to include the quality of conservation values. The results showed that lessons were effective in growing the value of conservation characters within the student by the indicator: (1) the results of self-assessment (self-assessment) was 94.07% of the students agreed and strongly agreed with the statement on the value of the existing character, (2) oral communication skills of students in storytelling by picture media was 70% of students have a performance very well and good, and (3) the response of students to the implementation of the study was 97% of the students found the strategy paired storytelling by picture media could train the students ability to observe and analyze the image and retell to friends written or orally.

Phylogenetic Relationships of Local Durian Species based on Morphological Characteristics and PCR-RFLP Analysis of the Ribosomal Internal Transcribed Spacer (ITS) DNA Fibriana, Fidia; Hadiyanti, Lutfia Nur
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6748

In this study, twenty local durian accessions obtained from Central Java in situ collection were characterized using the morphological characteristics and the restriction patterns which generated from the region spanning the internal transcribed spacers ITS LEU and ITS 4. Morphological characteristics of durian leaf, stem, tree, and fruit showed variations for the different accessions, whereas polymerase chain reaction (PCR) products of ribosomal DNA region showed a low length of variation. The size of the PCR products and the restriction analyses with the restriction endonucleases Bsp1431yielded a restriction pattern for each accessions. The results of this study can be utilized by local durian farmers as a preliminary reference for durian propagation. The data obtained need to be supported by further research using the other molecular markers to obtain more accurate data. The clear identity of durian species can help the management of propagation systems by farmers to get superior local durian.How to CiteFibriana, F., & Hadiyanti, L. N. (2016). Phylogenetic Relationships of Local Durian Species based on Morphological Characteristics and PCR-RFLP Analysis of the Ribosomal Internal Transcribed Spacer (ITS) DNA. Biosaintifika: Journal of Biology & Biology Education, 8(3), 362-370.

Deteksi Daging Babi Pada Produk Bakso di Pusat Kota Salatiga Menggunakan Teknik Polymerase Chain Reaction Fibriana, Fidia; Widianti, Tuti; Retnoningsih, Amin; -, Susanti
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 2 (2012): September 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i2.3928

Teknik PCR mempunyai kemampuan yang sensitif untuk deteksi keberadaan daging babi di dalam daging segar maupun produk olahan yang dicampur dengan bahan lain. Tujuan penelitian ini untuk mengetahui apakah produk bakso yang dijajakan di pusat kota Salatiga mengandung daging babi. Teknik stratified random sampling digunakan untuk mengambil sampel bakso yang dijajakan 13 warung bakso dari 25 warung bakso yang tersebar di pusat Kota Salatiga. Isolasi dan purifikasi DNA sampel bakso, daging sapi, dan daging babi menggunakan metode isolasi DNA jaringan hewan. DNA hasil isolasi dilanjutkan proses PCR menggunakan primer p14 untuk mengamplifikasi lokus PRE-1 pada genom babi. Proses amplifikasi DNA dengan program denaturasi awal pada suhu 93 C selama 2 menit, diikuti 45 siklus terdiri atas denaturasi 93 C selama 1 menit, annealing 62 C selama 30 detik, ekstensi 72 C selama 1 menit, kemudian diakhiri ekstensi 72 C selama 2 menit. Produk PCR yang diharapkan muncul berukuran 481bp. Hasil elektroforesis gel agarose 1,2% pada produk PCR menunjukkan adanya pita DNA spesifik berukuran 481 bp pada daging babi dan sampel bakso nomor tiga belas, sehingga disimpulkan warung bakso nomor tiga belas produk baksonya mengandung daging babi.PCR technique has the ability to be sensitive to the detection of the presence of pork in fresh meat and processed products are mixed with other materials. The aim of this research to determine whether the product meatballs are sold in downtown Salatiga containing pork. Stratified random sampling technique is used to take samples of meatballs stall which sold 13 of the 25 meatballs stalls in the Salatiga City centre. Isolation and purification of DNA samples of meatballs, beef, and pork using DNA isolation method of animal tissue. DNA isolation results continue the process of PCR using primers to amplify p14 locus PRE-1 in the pig genome. DNA amplification process with initial denaturation program at a temperature of 93 C for 2 min, followed by 45 cycles consisting of denaturation 93 C for 1 min, annealing 62 C for 30 seconds, extension 72 C for 1 minute, then topped extension 72 C for 2 minutes. PCR products were expected to appear sized 481bp. Results of a 1.2% agarose gel electrophoresis of PCR products indicate a specific DNA band sized 481 bp on pork and meatball sample number thirteen, so it concluded meatball stall number thirteen baksonya products containing pork.

Proteases from Latex of Euphorbia spp. and Its Application on Milk Clot Formation Fibriana, Fidia; Upaichit, Apichat
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.3951

Crude proteases were extracted from Euphorbiaceae family, i.e. E. milii var imperata, E. trigona, and E. maculata. Among those three crude proteases, the activity of protease from E. trigona was the highest (812.50 U/ml), whereas E. milii and E. maculata crude proteases activity were 298.60 U/ml and 95.80 U/ml, respectively. E. maculata protein concentration was the highest among those three crude enzymes (1.206 mg/ml). The optimum pH and temperature of the enzymes were pH 7.0, pH 6.0, pH 6.5 and 60 C, 50 C, and 50 C, respectively. Crude protease from E. milii var imperata, E. trigona, and E. maculata retained proteolytic activity over a wide range of pH (5.09.0) and temperature (up to 65 C) with casein as substrate. All crude proteases showed milk clotting activity ranged from 0.58 U/ml to 1.01 U/ml. Thus, these crude proteases are potential to be applied in dairy industries. However, further study on enzyme purification and characterization are necessary to obtain high purity of proteases before its application.Protease kasar berhasil diekstrak dari tanaman family Euphorbiaceae, yaitu E. milii var imperata, E. trigona, dan E. maculata. Diantara ketiga protease tersebut, aktivitas protease tertinggi diperoleh dari E. trigona (812,50 U/ml), sedangkan aktivitas protease dari E. milii dan E. maculata adalah 298,60 U/ml dan 95,80 U/ml, berturut-turut. Konsentrasi total protein tertinggi terdapat pada protease kasar E. maculata (1,206 mg/ml). pH dan suhu optimum ketiga enzim tersebut adalah pH 7.0, pH 6.0, pH 6.5 dan suhu 60 C, 50 C, and 50 C, berturut-turut. Protease kasar dari E. milii var imperata, E. trigona, dan E. maculata menunjukkan aktivitas proteolitik pada rentang pH 5.09.0 dan rentang suhu sampai 65 C menggunakan kasein sebagai substrat. Semua protease kasar menunjukkan aktivitas penggumpalan susu dengan rentang dari 0,58 U/ml sampai 1,01 U/ml. Berdasarkan hasil yang diperoleh, protease kasar dari ketiga jenis tanaman ini berpotensi untuk diaplikasikan dalam industri olahan susu. Meskipun demikian, studi lanjut mengenai purifikasi dan karakterisasi sangat diperlukan untuk memperoleh protease murni sebelum aplikasi dalam industri makanan, khususnya pada industri olahan susu.

PCR-RFLP Analysis of D-Loop mtDNA in Indonesian Domestic Waterfowl Susanti, R; Fibriana, Fidia; Yuniastuti, Ari
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.12177

Phylogenetic or population genetic studies have been focused on finding genetic variations in mitochondrial DNA (mtDNA) because mutations in this region contained five times higher than other genetic materials. The aim of this research was to generate PCR-RFLP D-loop mtDNA profiles of some Indonesian domestic waterfowl. The samples were 35 local ducks, 5 muscovy duck, and 5 geese. These local ducks included on seven types of Javanese ducks in Central Java, i.e. Magelang duck, Peking duck, Pengging duck, Tegal Branjangan duck, Tegal Jarakan duck, Tegal Blorong duck, and Tegal Lemahan duck. PCR technique was used to amplify the D-loop genes, and RFLP analysis of the D-loop was performed with AluIand RsaI. The results show that muscovy duck has no restriction sites in D-loop gene. Moreover, goose has a restriction site of AluI in D-loop gene at 550 bp and 187 bp; and it has no restriction site of RsaI. The analysis of RsaI-RFLP in mtDNA D-loop region of ducks resulted in 1 restriction fragment pattern which can be applied in the identification of duck to differentiate it from goose and muscovy duck. PCR-RFLP analysis duck D-loop mtDNA using AluI and RsaI restriction enzyme resulted in 3 combinations of restriction fragment pattern shown in 3 haplotypes (A, B, and C). Genetic diversity of Indonesian domestic waterfowl population could be identified by using PCR-RFLP analysis on mtDNA D-loop region. So far, there are few results of research on Indonesian domestic waterfowl genetic characteristics based on PCR-RFLP mtDNA D-loop marker. Also, this research has a good contribution on genetic characterization of local duck. The characterization is an important aspect to maintain breed quality and manage the genetic resources of Indonesian germplasm and in facilitating the breeding program.

POTENSI KITCHEN MICROBIOLOGY UNTUK MENINGKATKAN KETERAMPILAN TEKNIK HANDS-ON DALAM PEMBELAJARAN MIKROBIOLOGI Fibriana, Fidia; Amalia, Andin Vita
Unnes Science Education Journal Vol 5 No 2 (2016): July 2016
Publisher : Department of Integrated Science, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang in Collaboration with Perkumpulan Pendidikan IPA Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/usej.v5i2.12591

Abstrak___________________________________________________________________Peserta didik di sekolah menengah maupun perguruan tinggi perlu dibekali dengan kemampuan softskill dan hardskill sebagai bekal menghadapi tantangan yang semakin berat seiring dengan arus globalisasi yang cepat. Keterampilan hands-on konten mikrobiologi merupakan suatu keterampilan yang harus dikuasai oleh peserta didik khususnya dari bidang ilmu pengetahuan alam, biologi, kimia, farmasi, kedokteran, lingkungan, pangan, ilmu gizi, kesehatan masyarakat, pertanian, serta arkeologi. Akan tetapi, pembelajaran konten mikrobiologi di banyak sekolah menengah dan perguruan tinggi masih memiliki keterbatasan fasilitas dan biosafety yang menghalangi peserta didik untuk belajar dengan maksimal. Untuk itu diperlukan suatu teknik alternatif yang dapat diaplikasikan di sekolah menengah dan perguruan tinggi yang minim fasilitas dan memiliki keterbatasan biosafety. Dalam artikel ini akan dibahas mengenai teknik kitchen microbiology serta potensi aplikasinya dalam pembelajaran konten mikrobiologi khususnya dalam praktik hands-on. Selain itu, teknik ini memiliki potensi efektivitas yang baik untuk menumbuhkan kemampuan berpikir kritis dengan pendekatan pembelajaran berbasis inkuiri, kontekstual, maupun evidence-based approach.Â Abstract_______________________________________________________________The students of secondary school and higher education must be equipped with softskill and hardskill to face the global challenge in present and in the future. The hands-on skill of microbiology is a must have skill for students who are majoring in natural sciences, biology, chemistry, pharmacy, medical, environment, food, nutrition, public health, farming, and archeology. However, the microbiology learning in the secondary school and in the higher education level is still facing with the limitation of facilities and low level of biosafety. It prevents the students to fully comprehend the microbiology content. In this article, it will be explained about the effectiveness potency of kitchen microbiology technique to build the studentsâ€™ critical thinking ability and their hands-on skill on microbiology by the approach of inquiry-based and evidence-based.

PENERAPAN STRATEGI STORY TELLING DALAM PEMBELAJARAN BIOLOGI MOLEKULER BERBASIS KOMPETENSI DAN KONSERVASI Mustikaningtyas, Dewi; Widianti, Tuti; Fibriana, Fidia
Journal of Biology Education Vol 5 No 1 (2016)
Publisher : FMIPA UNNES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbe.v5i1.14667

Telah dilakukan penelitian pembelajaran Biologi Molekular berbasis Kompetensi dan Konservasi menggunakan strategi Paired Story telling with picture untuk mendiskripsikan bentuk pembelajaran yang dapat menumbuhkembangkan nilai-nilai konservasi dalam diri mahasiswa serta mengetahui efektivitasnya. Subyek penelitian adalah mahasiswa program studi Pendidikan Biologi berjumlah 42 orang angkatan 2013 yang telah menggunakan kurikulum Unnes 2012. Pembelajaran Biologi molekular berbasis kompetensi dan konservasi didiskripsikan sebagai pembelajaran yang mengintegrasikan materi dengan nilai karakter konservasi menggunakan strategi paired storytelling with pictures dengan terlebih dahulu melakukan analisis Standar Kompetensi (SK) dan Kompetensi Dasar (KD) dan pemetaan materi ajar yang memiliki korelasi dengan tema karakter atau nilai-nilai moral dalam konservasi, yang selanjutnya dirumuskan indikator pencapaian SK, KD dengan menyertakan kualitas nilai-nilai konservasi. Pembelajaran yang dilakukan efektif dalam menumbuhkan nilai-nilai karakter dalam diri mahasiswa dengan indikator: (1) hasil penilaian diri (self assessment) 94,07% mahasiswa setuju dan sangat setuju dengan pernyataan tentang nilai karakter yang ada, (2) kemampuan komunikasi lisan mahasiswa dalam storytelling with picture70% mahasiswa memiliki performance sangat baik dan baik, dan (3) tanggapan mahasiswa terhadap pelaksanaan pembelajaran 97% mahasiswa berpendapat bahwa strategi pairedstory telling with pictures melatih kemampuan mahasiswa mengamati dan menganalisis gambar dan menceritakan kembali kepada teman secara tertulis maupun lisan. Molecular Biology and conservation competency-based described as learning that integrates the material with the character of the conservation value using paired strategy storytelling with pictures by analyzing the standard of competence and basic competence. Subjects in this study were 42 students of Biology Education year 2013 who have used the 2012 Unnes curriculum learning. Further, mapping of teaching materials that have a correlation with the theme of character or moral values in conservation was performed. Then, it indicators was formulated for achievement of standard of competence and basic competence to include the quality of conservation values. The results showed that lessons were effective in growing the value of conservation characters within the student by the indicator: (1) the results of self-assessment (self-assessment) was 94.07% of the students agreed and strongly agreed with the statement on the value of the existing character, (2) oral communication skills of students in storytelling by picture media was 70% of students have a performance very well and good, and (3) the response of students to the implementation of the study was 97% of the students found the strategy paired storytelling by picture media could train the students' ability to observe and analyze the image and retell to friends written or orally.

POTENSI KITCHEN MICROBIOLOGY UNTUK MENINGKATKAN KETERAMPILAN TEKNIK HANDS-ON DALAM PEMBELAJARAN MIKROBIOLOGI Fibriana, Fidia; Amalia, Andin Vita
Unnes Science Education Journal Vol 5 No 2 (2016): July 2016
Publisher : Department of Integrated Science, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang in Collaboration with Perkumpulan Pendidikan IPA Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/usej.v5i2.12591

Abstrak___________________________________________________________________Peserta didik di sekolah menengah maupun perguruan tinggi perlu dibekali dengan kemampuan softskill dan hardskill sebagai bekal menghadapi tantangan yang semakin berat seiring dengan arus globalisasi yang cepat. Keterampilan hands-on konten mikrobiologi merupakan suatu keterampilan yang harus dikuasai oleh peserta didik khususnya dari bidang ilmu pengetahuan alam, biologi, kimia, farmasi, kedokteran, lingkungan, pangan, ilmu gizi, kesehatan masyarakat, pertanian, serta arkeologi. Akan tetapi, pembelajaran konten mikrobiologi di banyak sekolah menengah dan perguruan tinggi masih memiliki keterbatasan fasilitas dan biosafety yang menghalangi peserta didik untuk belajar dengan maksimal. Untuk itu diperlukan suatu teknik alternatif yang dapat diaplikasikan di sekolah menengah dan perguruan tinggi yang minim fasilitas dan memiliki keterbatasan biosafety. Dalam artikel ini akan dibahas mengenai teknik kitchen microbiology serta potensi aplikasinya dalam pembelajaran konten mikrobiologi khususnya dalam praktik hands-on. Selain itu, teknik ini memiliki potensi efektivitas yang baik untuk menumbuhkan kemampuan berpikir kritis dengan pendekatan pembelajaran berbasis inkuiri, kontekstual, maupun evidence-based approach.Â Abstract_______________________________________________________________The students of secondary school and higher education must be equipped with softskill and hardskill to face the global challenge in present and in the future. The hands-on skill of microbiology is a must have skill for students who are majoring in natural sciences, biology, chemistry, pharmacy, medical, environment, food, nutrition, public health, farming, and archeology. However, the microbiology learning in the secondary school and in the higher education level is still facing with the limitation of facilities and low level of biosafety. It prevents the students to fully comprehend the microbiology content. In this article, it will be explained about the effectiveness potency of kitchen microbiology technique to build the studentsâ€™ critical thinking ability and their hands-on skill on microbiology by the approach of inquiry-based and evidence-based.

THE ANALYSIS OF STAKEHOLDER SATISFACTION LEVEL TOWARDS THE INTEGRATED SCIENCE EDUCATION GRADUATES Amalia, Andin Vita; Dewi, Novi Ratna; Savitri, Erna Noor; Fibriana, Fidia
Unnes Science Education Journal Vol 7 No 3 (2018): December 2018
Publisher : Department of Integrated Science, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang in Collaboration with Perkumpulan Pendidikan IPA Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/usej.v7i3.27518

There has been limited information about the integrated science education alumni and the graduate user (stakeholder) satisfaction level. Therefore, a concrete step needs to be taken by the department managers in the form of alumni data collection â€” this research intended to reveal the stakeholders' responses to the Integrated Science Education graduates of Universitas Negeri Semarang. The methods adopted were descriptive-qualitative and quantitative employing questionnaire instruments available on the tracer study web system. The questionnaires had several indicators including (1) ethics and moral, (2) professionalism, (3) insight, (4) leadership, (5) teamwork, (6) foreign language mastery, (7) communication, (8) IT skills, and (9) self-development. Based on the accomplishment score average of the 9 indicators, the graduates categorized as very good having the final score of 3.72. The knowledge background suitability with the jobs scored 3.60 or categorized as very good. The analysis results revealed that of the 9 indicators, 7 of them categorized as â€˜very high' (ethics and moral, insight, leadership, teamwork, communication, IT skills, and self-development) and 2 of them categorized as â€˜high' (professionalism and foreign language mastery). The integrated science education alumni's suitability of competencies towards the jobs also categorized as â€˜very high.'

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