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All Journal Indonesian Biodiversity Journal Dharma Acariya Nusantara: Jurnal Pendidikan, Bahasa dan Budaya Indonesian Biodiversity Journal Pentagon: Jurnal Matematika dan Ilmu Pengetahuan Alam
Carolin Manuahe
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary

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ISOLATION AND IDENTIFICATION OF ENTOMOPATHOGENIC ON CROSIDOLOMIA BINOTALIS [ZELLER] (LEPIDOPTERA : PHYRALIDAE) USING 16S rRNA GENE: ISOLATION AND IDENTIFICATION OF ENTOMOPATHOGENIC ON CROSIDOLOMIA BINOTALIS [ZELLER] (LEPIDOPTERA : PHYRALIDAE) USING 16S rRNA GENE Carolin Manuahe
Indonesian Biodiversity Journal Vol. 2 No. 1 (2021): April 2021
Publisher : Universitas Negeri Manado

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Abstract

The aims of this reserach were to isolating and to identifying bacteria entomopathogenic on caterpillar pests in cabbage. The study was conducted in three phases. The first stage isolation of bacteria from cabbage caterpillar pests, the second stage of microscopic characterization of bacterial isolates and the third phase molecular identification of bacteria isolates using 16S RNA gene. The results were obtained five bacterial isolates of caterpillar pests in cabbage which 4 isolates shaped cocus, three isolates of gram positive and gram negative two isolates. All of isolates were able to degrade D-glucose. Isolates were translucent white color, white yellowish and brownish white. Generally isolates are not motile dan growth in the media to the surface. Results of DNA extraction and amplification of 16S RNA gene, showing the purity and concentration of DNA is good, confirmed by electrophoresis, amplicons formed band at 1500 bp according to the characteristics of the 16 S RNA gene
Aedes Mosquito Population density of Dengue Fever Vectors in The Area of Pineleng Minahasa: Aedes Mosquito Population density of Dengue Fever Vectors in The Area of Pineleng Minahasa Carolin Manuahe; Yermia Semuel Mokosuli; Ferny Margo Tumbel
Indonesian Biodiversity Journal Vol. 1 No. 1 (2020): April 2020
Publisher : Universitas Negeri Manado

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Abstract

Aedes mosquitoes have the behavior of sucking blood repeatedly (multiple bites) in one gonotrophic cycle, these mosquitoes are also very effective at transmitting the virus to humans (WHO 2004). Kawada et al. (2007) reported that the foraging activity of A. albopictus was less than 0.1 times compared to A. aegypti under laboratory conditions, this result is consistent with the tendency to bite both species in nature. The target-attack frequency (foraging activity) in A. aegypti female mosquitoes in unfed conditions is 30 times greater than that of A. albopictus mosquitoes in several laboratory treatments (Kawada, et al. 2007). Aedes sp is a deadly infectious vector between another dengue hemorrhagic fever (DHF) and chikungunya. Pineleng is a marginal/suburban area of Station 1. Recent studies have reported changes in habitat patterns of Aedes sp, especially Aedes albopictus and Aedes aegypti . The aim of this study is to characterize the population density of the Aagoan sp. Imago based on the time distribution and altitude of the sea surface. The population density characteristics of the Aedes spagoed imago captured inside and outside the room based on the time distribution in North Sulawesi. The stages of the study consisted of larvae surveying, larval extraction and analysis, and imago extraction and analysis. The results showed that the high population of Ae aegypti was at 8:00 a.m. to 10:00 p.m. in the morning and 14:00 to 16:00 p.m. in the afternoon before the afternoon. in the morning before noon and at 14:00 to 18:00 in the afternoon before evening.
ISOLATION AND IDENTIFICATION OF ENTOMOPATHOGENIC ON CROSIDOLOMIA BINOTALIS [ZELLER] (LEPIDOPTERA : PHYRALIDAE) USING 16S rRNA GENE: ISOLATION AND IDENTIFICATION OF ENTOMOPATHOGENIC ON CROSIDOLOMIA BINOTALIS [ZELLER] (LEPIDOPTERA : PHYRALIDAE) USING 16S rRNA GENE Carolin Manuahe
Indonesian Biodiversity Journal Vol. 2 No. 1 (2021): April 2021
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v2i1.137

Abstract

The aims of this reserach were to isolating and to identifying bacteria entomopathogenic on caterpillar pests in cabbage. The study was conducted in three phases. The first stage isolation of bacteria from cabbage caterpillar pests, the second stage of microscopic characterization of bacterial isolates and the third phase molecular identification of bacteria isolates using 16S RNA gene. The results were obtained five bacterial isolates of caterpillar pests in cabbage which 4 isolates shaped cocus, three isolates of gram positive and gram negative two isolates. All of isolates were able to degrade D-glucose. Isolates were translucent white color, white yellowish and brownish white. Generally isolates are not motile dan growth in the media to the surface. Results of DNA extraction and amplification of 16S RNA gene, showing the purity and concentration of DNA is good, confirmed by electrophoresis, amplicons formed band at 1500 bp according to the characteristics of the 16 S RNA gene
Aedes Mosquito Population density of Dengue Fever Vectors in The Area of Pineleng Minahasa: Aedes Mosquito Population density of Dengue Fever Vectors in The Area of Pineleng Minahasa Carolin Manuahe; Yermia Semuel Mokosuli; Ferny Margo Tumbel
Indonesian Biodiversity Journal Vol. 1 No. 1 (2020): April 2020
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v1i1.151

Abstract

Aedes mosquitoes have the behavior of sucking blood repeatedly (multiple bites) in one gonotrophic cycle, these mosquitoes are also very effective at transmitting the virus to humans (WHO 2004). Kawada et al. (2007) reported that the foraging activity of A. albopictus was less than 0.1 times compared to A. aegypti under laboratory conditions, this result is consistent with the tendency to bite both species in nature. The target-attack frequency (foraging activity) in A. aegypti female mosquitoes in unfed conditions is 30 times greater than that of A. albopictus mosquitoes in several laboratory treatments (Kawada, et al. 2007). Aedes sp is a deadly infectious vector between another dengue hemorrhagic fever (DHF) and chikungunya. Pineleng is a marginal/suburban area of Station 1. Recent studies have reported changes in habitat patterns of Aedes sp, especially Aedes albopictus and Aedes aegypti . The aim of this study is to characterize the population density of the Aagoan sp. Imago based on the time distribution and altitude of the sea surface. The population density characteristics of the Aedes spagoed imago captured inside and outside the room based on the time distribution in North Sulawesi. The stages of the study consisted of larvae surveying, larval extraction and analysis, and imago extraction and analysis. The results showed that the high population of Ae aegypti was at 8:00 a.m. to 10:00 p.m. in the morning and 14:00 to 16:00 p.m. in the afternoon before the afternoon. in the morning before noon and at 14:00 to 18:00 in the afternoon before evening.
Application Of The Inquiry Learning Model In Improving Student Learning Outcomes In Biology Science Learning At SMP Negeri 6 Tondano Sri Dewinta Kurais; Meity N. Tanor; Carolin Manuahe
Dharma Acariya Nusantara: Jurnal Pendidikan, Bahasa dan Budaya Vol. 1 No. 2 (2023): SEPTEMBER: Jurnal Pendidikan, Bahasa dan Budaya
Publisher : Sekolah Tinggi Agama Buddha Nalanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47861/jdan.v1i2.485

Abstract

Inquiry-based learning refers to a sequence of educational activities that aim to fully engage students in methodical, critical, logical, and analytical processes of searching and investigating. The ultimate goal is for students to independently develop their own discoveries with a high level of confidence. This study aims to find out the students' mastery learning results by using the inquiry learning model in which the material is related to the structure and function of plant parts. This research is classroom action research. This research was conducted at SMP Negeri 6 Tondano. The research subjects were class VIII students, totaling 27 people. The results showed that the percentage of learning completeness in cycle I was 29.63% with an average score of 60.62, and cycle II was 92.60% with an average score of 81.07. The results of this study indicate that learning the structure and function of plant parts using the inquiry learning model can improve student learning outcomes.
Improving Biology Learning Outcomes on the Subject of Ecology Using Think Pair Share Type Learning Strategies for Class X Science Students at Onate High School, Yapen Islands, Papua Province Martenci Yawandare; Orbanus Naharia; Carolin Manuahe
Pentagon : Jurnal Matematika dan Ilmu Pengetahuan Alam Vol. 2 No. 2 (2024): Juni : Pentagon : Jurnal Matematika dan Ilmu Pengetahuan Alam
Publisher : Asosiasi Riset Ilmu Matematika dan Sains Indonesia

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Abstract

This research aims to improve student learning outcomes on ecology by implementing the Think Pair Share type learning strategy in class X Science at SMA Onate, Yapen Islands, Papua Province. The research method used is classroom action research. The subjects of this research were 25 class X Science students at Onate High School, Yapen Islands, Papua Province. This research was carried out in two cycles, and each cycle was carried out in 3 meetings. The research results showed that the recapitulation of learning outcomes in cycle I was 60%, or 15 students completed classical learning. In comparison, in cycle II, the percentage of student learning completion was 88%, or 22 students completed classical learning. Implementing the Think Pair Share type learning strategy can improve student learning outcomes in class X Science at SMA Onate, Yapen Islands, Papua Province.
Co-Authors Ferni Margo Tumbel Ferny Margo Tumbel Martenci Yawandare Meity Nelltje Tanor Mokosuli Yermia Samuel Orbanus Naharia Sri Dewinta Kurais