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All Journal Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Agronomi Tanaman Tropika (JUATIKA) Bioscientist : Jurnal Ilmiah Biologi
Herry Nirwanto
Universitas Pembangunan Nasional "Veteran" Jawa Timur
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Engineering Immunology & microbiology Social Sciences

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Study of Thermal Imaging Potential for Early Detection of Fusarium sp. Pathogen on Rice Seeds (Oryza sativa L.) Avianita Agustianti; Herry Nirwanto; Maroeto Maroeto
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 15 No. 1 (2026): February 2026
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtepl.v15i1.90-100

Abstract

The early detection of Fusarium sp. infection in rice seeds is crucial for improving agricultural productivity and food security. Traditional methods like the Blotter Test, while effective, are time-consuming and require specialized personnel. This study explores the potential of thermal imaging technology to detect Fusarium sp. infections on rice seeds quickly and non-destructively. Rice seeds were inoculated with Fusarium sp. and incubated for seven days, during which surface temperatures were measured daily using the Fluke iSee TC01A thermal camera. The results showed that infected seeds exhibited significantly higher surface temperatures compared to control seeds, particularly from days 3 to 6 of incubation. Scatterplot analyses revealed clear temperature differences between infected and uninfected seeds, supporting the hypothesis that thermal imaging can serve as an early indicator of Fusarium infection. The study also demonstrated the high sensitivity and specificity of thermal imaging, particularly on days 2 to 4 of the incubation period. Logistic regression analysis confirmed the significant relationship between seed temperature and infection status, with prediction accuracy up to 91%. This research suggests that thermal imaging technology could replace traditional methods, offering a faster, more efficient approach for seed health monitoring in the agricultural industry.
Fluctuations in Fruit Fly Population (Bactrocera spp.) and Its Relationship to Environmental Conditions in Red Guava Plantations in Sidoarjo Regency Indra Djiwanata; Noni Rahmadhini; Herry Nirwanto
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol. 8 No. 1 (2026): Jurnal Agronomi Tanaman Tropika (JUATIKA) Vol. 8 No. 1 January 2026
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v8i1.4706

Abstract

Crystal guava (Psidium guajava) is a superior variety cultivated in Indonesia. A major constraint in crystal guava production is the high incidence of pest attacks, particularly from fruit flies. This study was conducted in a red guava orchard located in Sidoarjo Regency and Jalan Simorejosari A IV/17 during the period from July to August. The research employed a survey method involving the installation of attractant traps and purposive sampling to collect infested fruit for host rearing. Both trapping and host rearing were used to monitor the fruit fly population. Trapping was carried out by installing attractant traps at five predetermined plots, positioned at a height of 1.5 meters and spaced 20 meters apart. Fruit fly rearing involved collecting infested fruit using purposive sampling from the same five plots, then incubating until adult flies emerged. The fruit fly species attacking the red guava orchard was identified as Bactrocera dorsalis. Independent t-test analysis revealed significant differences between the populations of trapped fruit flies and those obtained from host rearing. The emergence rate of fruit fly adults from host rearing exhibited a negative correlation with the trapped fruit fly population, described by the regression equation Y = 359.282 – 7.2146X. Path analysis indicated that rainfall positively correlates with temperature and humidity, while temperature negatively correlates with humidity. Additionally, rainfall and humidity negatively correlate with the trapped fruit fly population, whereas temperature shows a positive correlation.
Effectiveness of Antagonistic Agents from Several Bacillus spp. Isolates against Anthracnose Disease (Colletotrichum capsici) in Chili Pepper Fruit (Capsicum annuum L.) Aullyvia Putri Nabiella; Yenny Wuryandari; Herry Nirwanto
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20692

Abstract

This study aimed to evaluate the antagonistic activity of selected Bacillus spp. isolates against Colletotrichum capsici and determine the most effective isolate and cell density for suppressing anthracnose in red chili pepper. The study was conducted under in vitro and in vivo conditions. The in vitro assay tested five Bacillus spp. isolates, namely Bcz-14, Bcz-16, Bcz-20, Bcz-21, and Bcz-30, at cell densities of 10⁹ and 10¹⁰ CFU/mL. The three most effective treatments from the in vitro assay were subsequently evaluated under in vivo conditions. Data were analyzed using SPSS version 24 through analysis of variance (ANOVA) at a 5% significance level, followed by Tukey’s honestly significant difference (HSD) test at 5% when significant differences were detected. The results showed that B1K1 treatment (Bcz-14 at 10⁹ CFU/mL), with an inhibition zone of 5.83 mm; B5K1 treatment (Bcz-30 at 10⁹ CFU/mL), with an inhibition zone of 5.50 mm; and B2K1 treatment (Bcz-16 at 10⁹ CFU/mL), with an inhibition zone of 4.16 mm, exhibited the most stable inhibitory effects against C. capsici growth in vitro. The in vivo assay demonstrated that B1K1 treatment (Bcz-14 at 10⁹ CFU/mL) was the most effective treatment, resulting in a disease intensity of 26.25% and a control efficacy of 51.16%.
Co-Authors Aullyvia Putri Nabiella Avianita Agustianti Indra Djiwanata Maroeto Maroeto Noni Rahmadhini Yenny WURYANDARI