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Analisa Kandungan Mineral, Lemak dan Protein pada Sampel Ikan Kembung Rebus Asin Leni Widiarti; Sajaratud Dur
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 6, No 1 (2022): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v6i1.12407

Research has been carried out to analyze the content of minerals, fat and protein in salted boiled pufferfish samples. Samples were purchased from a traditional market in Medan City, Indonesia. The method used is the 2005 AOAC method. Crude protein analysis was carried out by the Kjeldhal method, crude fat analysis was carried out by the Soxhlet extraction method, water content analysis was carried out by the drying method, ash content analysis was carried out by the combustion method at high temperature, and mineral analysis was carried out by using an Atomic Absorption Spectrophotometer (AAS). The results of the analysis of the salted mackerel sample obtained crude protein content of 65.9770 %, this indicates that the salted boiled mackerel sample has a high protein content. Crude fat content from the analysis is 16.7600 %. The water content and ash content of the boiled mackerel samples were 9.5077% and 6.2700%, respectively. Mineral analysis, namely the levels of calcium and sodium obtained respectively 0.7635 ppm and 0.7571 ppm.

Analisa Kandungan Mineral, Lemak dan Protein pada Sampel Ikan Kembung Rebus Asin Leni Widiarti; Sajaratud Dur
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 6, No 1 (2022): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v6i1.12407

Research has been carried out to analyze the content of minerals, fat and protein in salted boiled pufferfish samples. Samples were purchased from a traditional market in Medan City, Indonesia. The method used is the 2005 AOAC method. Crude protein analysis was carried out by the Kjeldhal method, crude fat analysis was carried out by the Soxhlet extraction method, water content analysis was carried out by the drying method, ash content analysis was carried out by the combustion method at high temperature, and mineral analysis was carried out by using an Atomic Absorption Spectrophotometer (AAS). The results of the analysis of the salted mackerel sample obtained crude protein content of 65.9770 %, this indicates that the salted boiled mackerel sample has a high protein content. Crude fat content from the analysis is 16.7600 %. The water content and ash content of the boiled mackerel samples were 9.5077% and 6.2700%, respectively. Mineral analysis, namely the levels of calcium and sodium obtained respectively 0.7635 ppm and 0.7571 ppm.

SOIL QUALITY IN ORGANIC AGRICULTURAL LAND: STUDY OF CHEMICAL ANALYSIS AND SOIL MICROBIOLOGY Melfa Aisyah Hutasuhut; Husnarika Febriani; Leni Widiarti
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 9 No. 2 (2023): February
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v9i2.8178

Increased public awareness for a healthy diet must be balanced with successful cultivation. Organic farming system is the right choice since it leaves all non-organic components. This study aims to identify the chemical and microbiological properties of agricultural soils that apply organic systems located in Batang Buluh Village, Pematang Johar, Deli Serdang Regency, North Sumatra. Chemical analysis was carried out at Socfindo Laboratory in Medan, including testing the pH of H2O, total P and K, C Organic, N Kjehldahl, and CEC (Cation Exchange Capacity). Soil microbiological tests were carried out at Medan Regional Health Lab including gram staining tests and biochemical tests. The results of chemical analysis from the analysis of pH H2O, P and total K, Organic C, N Kjehldahl, and CEC (Cation Exchange Capacity) at the edges and middle each obtained that was pH 5 -6, soil total P content was 0.0260% up to 0.450%, available K analysis, namely 0.200% and 0.210%, organic C content obtained results of 0.970% and 0.630%, N content using the KJehldahl method was 0.150% and 0.090%, and CEC obtained results of 14.330 me/100 and 10.090 me/100 g. Related species of Bacillus contained in the soil were Gram-positive, rod-shaped, spore-forming, aerobic or facultative anaerobic, motile bacteria with peritrichous flagella.

Molecular Weight and Functional Group Analysis of Low Molecular Weight of Liquid Cyclic Natural Rubber Widiarti, Leni; Sajaratuddur, Sajaratuddur; Wirjosentono, Basuki; Eddyanto, Eddyanto
ALKIMIA Vol 6 No 1 (2022): ALKIMIA
Publisher : SCIENCE AND TECHNOLOGY FACULTY OF UNIVERSITAS ISLAM NEGERI RADEN FATAH PALEMBANG

The research about molecular weight and functional group analysis of low molecular weight liquid cyclic natural rubber has been done. The aim of this research is to achievement the quality of low molecular weight of liquid cyclic natural rubber. This research has been made in several steps such as preparation of the sample of rubber, the process of molecular degradation of cyclic rubber, and characterization of LCNR by using FTIR, GPC and viscosity test. Degradation is done with the help of the phenylhydrazine reagent with oxygen gas atmosphere with a flow rate of 2 LMin-1 for 24 hours. Molecular weight analysis by GPC had result the LCNR sample had a Mw is 60,556, a Mn is 6,661, and a PDI is 11,08613. The intrinsic viscosity can be used by relating it to the molecular weight by the Mark Houwink – Sakurada (MHS) equation and get the result 63.533 for LCNR molecular weight. The C-H stretching and bending region are two of the most difficult regions to interpret in infrared spectra. The ranges between 3300 to 2750 cm-1 is the C-H stretching region, is the more practical of the two regions. The frequency with which C-H bonds are obstructed is largely determined by the type of hybridization attributed to the bond. The stronger the vibrational force constant, the higher the vibration frequency.

Effect of Moringa oleifera L. Leaf Extract on GPx-3 Gene Expression in the Brain of Induced Ethylene Glycol on Rats (Rattus norvegicus) Niska Maulida Nasution; Zahratul Idami; Leni Widiarti; Nurlian Augustin Ningrum
RUMPHIUS Vol 8 No 1 (2026): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv8i1p055-060

Ethylene glycol is one of the toxic substances commonly used as solvents and is known to induce oxidative stress in neural tissue. GPX-3 is an antioxidant enzyme that works to reduce ROS, maintain redox balance, and protect cells from damage caused by free radicals produced during oxidative stress processes. This study aims to evaluate the effect of administering Moringa oleifera L. leaf extract after ethylene glycol induction on the intensity of GPx-3 gene expression in the brain of white rats (Rattus norvegicus). This research employed a completely randomized design (RAL) using 20 rats divided into five groups: a normal control group, a 0.75% ethylene glycol group, a 150 mg/kgBW Mo group, a 300 mg/kgBW Mo group, and a 450 mg/kgBW Mo group. The research stages included brain RNA extraction, cDNA synthesis, PCR amplification, agarose gel electrophoresis, and band intensity analysis using ImageJ. The results showed that GPx-3 gene expression in rat brain tissue appeared at 300 bp. Administration of Moringa oleifera L. leaf extract increased GPx-3 gene expression intensity in ethylene glycol induced rats, with the most prominent increase observed in treatment 2 (300 mg/kgBW). The increase or decrease in measured expression after normalization to GAPDH indicates that the observed changes reflect a biological response to treatment rather than technical error. This study presents a novelty finding by demonstrating, for the first time, the dose-dependent modulation of GPx-3 gene expression in brain tissue following ethylene glycol induced oxidative stress and identifying 300 mg/kgBW as the most effective dose, thereby revealing a potential molecular mechanism underlying the neuroprotective effect of Moringa oleifera L. leaf extract.

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