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Evaluation of Semen Quality and Estimation of Semen Repeatability Value of Madura Bulls at Lembang Artificial Insemination Center Wigiyanti, Ema Trie; Indrijani, Heni; Darodjah, Siti; Kurnia, Asep
Jurnal Ilmu Ternak Universitas Padjadjaran Vol 24, No 2 (2024)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v24i2.53308

Abstract

Madura bull are one of the local bulls in Indonesia whose semen is used for artificial insemination. This study aims to evaluate the quality of semen and the repeatability value of semen from Madura bulls at the Lembang Artificial Insemination Center. A total of 1,402 semen collection data from three Madura bulls from 2014-2020 were used for analysis. The data was analyzed descriptively to evaluate semen quality consisting of semen volume, sperm motility and sperm concentration. Repeatability value was estimated using analysis of variance and intra-class correlation. The results showed that the average volume, motility, and concentration were 5.32 ± 1.50 ml; 68.10±7.07 %; and 1,044.27 ± 277.63 million/ml. The semen volume repeatability value was high, motility was low, and concentration was medium.
Reproductive Performance of Ongole Grade, Simmental, and Limousin Cattle Given Superovulation Treatment Pangestu, Muhamad Syahid Panji; Darodjah, Siti; Hilmia, Nena; Imron, Muhamad
Jurnal Agripet Vol 24, No 2 (2024): Volume 24, No. 2, October 2024
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v24i2.32335

Abstract

Reproductive technologies have the potential to enhance livestock productivity, with superovulation being one such method aimed at increasing the number of ovulated oocytes per estrous cycle through hormonal stimulation. This study aimed to evaluate the reproductive performance of three cattle breedsOngole Grade (OG) (n=20), Simmental (n=18), and Limousin (n=20)subjected to superovulation treatment. The cattle, aged 2.5 to 4 years with a body condition score of 3.5 to 4, were intensively managed. Superovulation was induced using CIDR (Controlled Internal Drug Release) for synchronization, followed by follicle-stimulating hormone (FSH) administration. Reproductive performance data, including age at first estrus, number of corpora lutea (CL), and embryo production, were collected over three estrous cycles. A quasi-experimental design was employed with breed as the primary treatment factor, and data were analyzed using analysis of variance (ANOVA). The results indicated that breed had a significant effect (P0.05) on the age of first estrus, with OG cattle reaching first estrus at 621.393.3 days, Simmental at 522.475.5 days, and Limousin at 570.488.1 days. However, no significant differences (P0.05) were observed among the breeds in terms of the number of CL, collected embryos, or transferable embryos across the superovulation cycles.
Effect of Various Avian Egg Yolk Types on the Viability and Longevity of Post-Thawed Resiquimod-Sexed Spermatozoa in Sapera Goats Setiadi, Deffany Wulandari; Solihati, Nurcholidah; Darodjah, Siti
Jurnal Produksi Ternak Terapan Vol 7, No 1 (2026): Volume 7 Nomor 1 Tahun 2026
Publisher : Faculty of Animal Husbandry, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jptt.v7i1.71539

Abstract

Improving the reproductive efficiency of Sapera goats through artificial insemination necessitates optimal quality of sexed frozen semen. This study aimed to determine the effect of various avian egg yolk types on the viability and longevity of post-thawed Sapera Goat spermatozoa sexed using the Resiquimod-based swim-up method. The research used a Completely Randomized Design (CRD) with four egg yolk types: Commercial Chicken (T1), Native Chicken (T2), Quail (T3), and Duck (T4) as treatments and five replications. Data were analyzed using One-Way ANOVA and Duncan’s Multiple Range Test. The results indicated that post-thaw viability ranged from 53.30–64.90% in the upper layer, while longevity ranged from 26.17–29.46 hours in the upper layer and 25.11–28.95 hours in the lower layer, showed no significant differences among treatments. Conversely, the egg yolk type significantly influenced viability in the lower layer (P < 0.05). Specifically, Native Chicken (T2), Duck (T4), and Commercial Chicken (T1) egg yolks maintained superior and stable viability (60.90%, 59.80%, and 56.20%), whereas Quail egg yolk (T3) exhibited significantly lower performance (52.40%). These findings suggest that the choice of egg yolk is critical for maintaining the sperm viability of the lower layer in Resiquimod-based sexed semen.