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All Journal Tropical Medicine Journal The Indonesian Biomedical Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Umi Solekhah Intansari
Department Of Clinical Pathology And Laboratory Medicine, Faculty Of Medicine, Nursing And Public Health, Gadjah Mada University, Yogyakarta
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience Public Health

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Journal : Tropical Medicine Journal

 1 
The HLA-DR Expression on Monocytes in Acute Dengue Infection Umi Solekhah Intansari; Muhammad Ilham Bin Azib; Mohd Nazirul Shareef bin Mohd Ridhwan; Ida Safitri Laksanawati
Tropical Medicine Journal Vol 2, No 1 (2012): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.945 KB) | DOI: 10.22146/tmj.4311

Abstract

Introduction: Dengue is the most common disease of mosquito-borne infection. According to the World Health Organization, it is estimated that 50 million cases ofdengue infection have been reported annually. Macrophages andmonocyte are thought to play an important role in dengue infection both as primary targets of viral infection and as a source of immunomodulatory cytokines.Whenmonocytes are infectedby dengue viruses, it processed the virus by lyses it and expressed the antigen on its surfacemembrane bind together with HLA class II molecules. HLA-DR-expressing monocytes in acute dengue patientsespecially the intensity is still poorly understood.Measurement of at difference days of infectionwill give an evidence about the role ofmonocytes in dengue pathogenesisObjectives: To observe the kinetics on percentage of HLA-DR expressingmonocytes and themedian of HLA-DR expression intensity in acute dengue infection.Methods: This researchwas an observational type study conducted by cross sectionalmethod towards all the dengue patients in Dr. Sardjito’s General Hospital. Blood samples were drawn from 32 acute dengue infected patients from Day 1 to Day 6th. The HLA-DR expression was measured flow cytometrically using FACS Calibur.Results: The data showed that the mean difference in acute dengue infection from Day 1 to Day 6 is not significant with the p-value larger than 0.05, (p>0.05).Conclusion: The peak level of activated HLA-DR monocyte was at day 2 and then decreasing until day 6. There were no significant changes in percentage of HLA-DR-expressing monocytes from the day 1 to the day 6 and the median intensity of HLA-DR expression in acute dengue infection.Keywords HLA-DR-expressing monocytes, Acute Dengue Infection
THE KINETIC OF ACTIVATED MONOCYTES IN ACUTE PHASE DENGUE INFECTION Umi Solekhah Intansari; Arif Bahiyuddin bin Badaruddin; Ida Safitri Laksanawati
Tropical Medicine Journal Vol 1, No 2 (2011): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1044.323 KB) | DOI: 10.22146/tmj.4572

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Introductions: Nowadays dengue infection is at present one of the most common mosquito-borne viral diseases of humans worldwide. Initially, Dengue infections were primarily recorded when they occurred as epidemics in tropical and subtropical countries. Monocyte/macrophage infection is central to the pathogenesis of dengue fever and to the origin of dengue hemorrhagic fever. Increased activation of monocytes and greater numbers of DEN-infected cells were associated with more severe Dengue, implicating a role for monocyte activation in dengue immunopathogenesis. Thus, more knowledge about the relation between numbers of activated monocyte with dengue severities is essential for better understanding regarding this subject.Objectives: Studying the relation between numbers of activated monocyte, marked by HLA-DR marker intensity with Dengue Fever severity throughout the disease course, from day 2 until day 6 of acute dengue fever.Methods: This research is cross sectional observational study. The location in done at Dr. Sardjito Hospital in 6 months time.Results: There is a negative correlation between median number of activated monocyte with dengue severity, on day 3 of acute dengue fever.Conclusion: There was a negative correlation between numbers of activated monocyte marked by HLADR intensity with disease severity on day 3 of acute dengue infection.Keywords: Activated Monocyte; Human Leukocyte Antigen (HLA)-DR intensity; Percentage of Activated Monocyte; Dengue Fever Severity.
The Kinetics of CD8+ T Lymphocytes in Dengue Patients in Yogyakarta Loo Huai Na; Umi Solekhah Intansari; Ida Safitri Laksanawati
Tropical Medicine Journal Vol 2, No 2 (2012): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.663 KB) | DOI: 10.22146/tmj.17127

Abstract

ABSTRACTIntroduction: Dengue    fever can be graded into dengue fever (DF), dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The CD8+ T lymphocytes mediate antiviral activity by producing cytokines and directly destroyed the dengue virus infected cells. This study focuses in observing the kinetics of CD8+ T lymphocytes absolute and relative count in dengue patients.Objectives: To observe the kinetics of CD8+ T lymphocytes absolute and relative              count in dengue patients.Methods: The research design used is a descriptive study. This research measures and observes the kinetics CD8+ T lymphocytes absolute and relative count from day 2 to day 7. The CD8+ T lymphocytes count was determined using flowcytometry. Data was analyzed using ANOVA and independent t test with p<0.05 considered as significant.Results: The CD8+ T lymphocytes absolute count is low during the beginning of disease course and it gradually increases from day 2 to day 7. The CD8+ T lymphocytes relative count decreases from day 2 to day 3, and start to increase back from day 3 to day 7. There is no difference between the level of CD8+ T lymphocytes absolute count and relative count between DF and DHF patients.Conclusion: There is an increase in CD8+ T lymphocytes absolute count and relative count in dengue patients. There is no difference between DF and DHF patients in CD8+ T lymphocytes absolute and relative count. Keywords: dengue fever; dengue hemorrhagic fever; CD8+ T lymphocytes; absolute count; relative count.
Co-Authors Arif Bahiyuddin bin Badaruddin Caesar Haryo Bimoseno Ida Safitri Laksanawati Loo Huai Na Mohd Nazirul Shareef bin Mohd Ridhwan Muhammad Ilham Bin Azib Purbosari Lisnaedy Purbosari Purbosari Surawijaya Bakhtiar Kaslam Teguh Triyono Yenny Surjawan