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Isolation and selection of siderophore-producing bacteria from roots of Simadu pineapple (Ananas comosus) in Subang District, West Java Hanifah FUADI; Prayoga SURYADARMA; Khaswar SYAMSU; Surono SURONO; Nurika Asih SETIYANI; Selvia Mahilda RIDHOHA; Aninda Sekar ZAHRA; Nadia STEPANI; Muhammad Rasyid RAMADHAN
E-Journal Menara Perkebunan Vol 90, No 2 (2022): Oktober, 2022
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v90i2.502

Abstrak Bakteri mampu menghasilkan siderofor umtuk mengkelat besi dalam lingkungan dan digunakan oleh tanaman sebagai kofaktor dalam pembentukan klorofil untuk pembentukan glukosa. Penelitian ini bertujuan untuk mendapatkan bakteri penghasil siderofor tinggi dari akar nanas Simadu, Kabupaten Subang Provinsi Jawa Barat. Bakteri diisolasi dari akar nanas Simadu, kemudian diseleksi dengan membedakan morfologinya dan diidentifikasi sebagai penghasil siderofor pada media Chrome Azurol S (CAS). Diperoleh 10 isolat bakteri (M1 sampai dengan M10) yang mampu menghasilkan siderofor. Isolat bakteri M7 memiliki kemampuan menghasilkan siderofor tertinggi. Isolat M7 teridentifikasi sebagai bakteri Gram negatif. Hasil analisis pohon filogenetik berdasarkan sekuensing 16S rDNA menunjukkan isolat kelompok genus Providencia. Dibandingkan dengan Providencia vermicola , isolat standar dari InaCC yang berasal dari akar Curcuma zedoaria, M7 menunjukkan produksi siderofor yang lebih tinggi pada media LB pada kondisi aerobik.[Kata kunci: Isolasi bakteri, Providencia, akumulasi siderofor, akar nanas Simadu Subang]Abstract Bacteria can produce siderophores for chelating iron in environments and are used by plants as an ingredient cofactor in building chlorophyll for glucose production. This study aims to obtain high siderophore-producing bacteria from the roots of pineapple Simadu, Subang District, West Java Province. Bacteria were isolated from Simadu pineapple roots, then selected by distinguishing their morphology and identified as producing siderophores with Chrome Azurol S (CAS) media. Obtained 10 bacterial isolates (M1 to M10) capable of producing siderophores. Bacterial isolate M7 had the highest siderophore production ability. M7 isolate was identified as Gram-negative bacteria. The results of the phylogenetic tree analysis based on 16S rDNA sequencing showed this isolate as the genus Providencia. Compared to the Providencia vermicola as reference isolate from InaCC derived from the roots of Curcuma zedoaria, M7 showed higher siderophore production in LB media under aerobic conditions.[Keywords: Bacteria isolation, Providencia, siderophore accumulation, Simadu Subang pineapple root]

Glycerol Utilization as Substrate for Ethanol Production in Escherichia coli Recombinant under an Aerobic Condition Wahyu Suradi Pranata; Prayoga Suryadarma; Djumali Mangunwidjaja
Journal of Tropical Life Science Vol. 8 No. 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.14

The utilization of glycerol as a substrate for ethanol production in E. coli recombinant harboring ethanologenic gens (PDC and ADHB) under aerobic conditions was investigated. This research was conducted by using E. coli BW25113 which compared the growth characteristics on glycerol and glucose as carbon source. E. coli can grow well on both substrates and it consumed glycerol faster than glucose. On glucose, E. coli occurred overflow metabolism that indicated by high acetate accumulation. Meanwhile, on substrate of glycerol the acetate accumulation could be reduced. Using glycerol substrate on E. coli increased piruvate accumulation, comparing with glucose. Therefore, the growth characteristic of glycerol was more effective. In E. coli âˆ†pta/pHfdh/pTadhB-pdc utilized glycerol substrate was able to accumulate pyruvate as intermediate metabolite for produce 2.18 gL-1 ethanol.

Dose and Time-Dependent Lipopolysaccharide Exposure on A549 Cell Model Influences Pro-Inflammatory Cytokine Interleukin 8 Sarah Sumayyah; Prayoga Suryadarma; Rachmawati Noverina; Wireni Ayuningtyas; Firman Fuad Wirakusumah; Ahmad Faried
Majalah Kedokteran Bandung Vol 55, No 2 (2023)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v55n2.3143

Hyperinflammation in COVID-19 patients is one of the causes of the high mortality rate of COVID-19. An in vitro model mimicking the inflammatory responses in COVID-19 patients is important in the efforts of finding new drug candidates for this disease. Lipopolysaccharide (LPS) can increase the proinflammatory cytokine interleukin 8 in response to the presence of foreign substances. This preliminary study sought to explore the use of the A549 cells as an in vitro inflammatory model. This study was conducted from August to November 2022 at the stem cell research and development laboratory of Bio Farma Indonesia. The exposure of 100, 500, and 1000 g/mL doses of LPS administered for 24, 72, and 120 hours on the A549 cells was analyzed for cell viability, population doubling time (PDT), and the presence of proinflammatory cytokine IL-8. The group differences were examined using one- and two-way analysis of variance in IBM SPSS Statistics Version 29, with a p-value of 0.05 considered significant. Cells exposed to a dose of 1000 g/mL LPS had a lower viability and a higher proliferation rate (p<0.05) based on the viability and PDT. Viability, PDT, and pro-inflammatory cytokines showed concentration- and time-dependent responses. Therefore, increased levels of the proinflammatory cytokine IL-8 in cells exposed to LPS at a dose of 1000 g/mL for 24 hours can be used as a mimic to study hyperinflammation in COVID-19 patients.

Evaluasi sifat fisik sediaan tablet dari ekstrak pedicel buah merah dan serbuk inulin komersial secara kempa langsung Prayoga Suryadarma; Murtiningrum Murtiningrum; Ani Suryani; Djumali Mangunwidjaja
AGROINTEK Vol 18, No 3 (2024)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v18i3.16948

Red fruit pedicel is a solid waste of the red fruit oil production. The pedicel contain inulin as dietary fiber. The inulin as an innovation that is practical and stable during storage. This study aimed to investigate the physical properties of dry inulin powder, inulin granules, and tablets from red fruit pedicel extract and compare them with commercial inulin. Inulin from red fruit pedicel was extracted using the hydrodynamic cavitation and dried using a spray dryer.. Then the inulin powder was added 20% maltodextrin with a ratio of 1:10. Preparation of inulin granules with a total volume of 100 mg was made with the composition of 54% of inulin powder, 25% of avicel, 0.1% of aerosil, 1% of magnesium stearate, and 19.9% of lactose. For inulin powder, the water content was evaluated; then, for the granules, flow rate, angle of repose, and compressibility were evaluated; and for tablets, the uniformity of weight, hardness, disintegration time, and friability were observed. The result showed that the water content of and commercial inulin were 7.78% and 5.42%, respectively. The flow rate, angle of repose, and compressibility of the red fruit pedicel extract granules were 1.91 g/second, 17.74o, and 27.04%, respectively. Meanwhile, the flow rate, angle of repose, and compressibility of commercial inulin were 11.31 g/second, 19.03o, and 41.03%, respectively. The average weight of the red fruit pedicel extract tablets and commercial inulin were 97.72 g and 97.53 g, respectively. The hardness, disintegration time, and friability of red fruit pedicel extract tablet were 11.26 kg/cm3, 13 minutes 12 seconds, and 0.63%, respectively. Then hardness, disintegration time, and friability of commercial inulin were 7.5 kg/cm3, 9 minutes 2 seconds, and 0.69%, respectively.

Peningkatan akumulasi tiamin pada kultur Escherichia coli melalui peningkatan oksigenasi dan penghilangan rpoS Nuraliah Rusman; Prayoga Suryadarma; Nisa Rachmania Mubarik
AGROINTEK Vol 19, No 1 (2025)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v19i1.11110

Microorganisms as cell factories in producing a product are strongly influenced by their metabolism. engineering microbial metabolism by increasing coenzymes that play a role in enzyme activity. Thiamine diphosphate is one of the coenzymes that are commonly found in the metabolic pathways of microbial cells. Increasing the thiamine formation pathway as a precursor in the thiamine metabolic pathway is a strategy in this study. Enhancing thiamine accumulation in Escherichia coli culture by inducing oxygenation level and deleting rpoS was investigated. The investigation was conducted by comparing the cultivation of Escherichia coli BW25113 (parent strain) and BW25113-∆rpoS (rpoS mutant) cultures under aerobic conditions at different shaking rotation rates of 150 and 200 rpm. The measured parameters were thiamine accumulation, dry cell weight, acetate concentration and glucose consumption of the cultures. Inducing oxygenation level of parent strain cultures through increasing shaking rotation rate from 150 to 200 rpm induced 40 % thiamine accumulation. Meanwhile, deletion of rpoS increased 9,5 % thiamine accumulation compared to parent strain culture

Isolation and selection of siderophore-producing bacteria from roots of Simadu pineapple (Ananas comosus) in Subang District, West Java Hanifah FUADI; Prayoga SURYADARMA; Khaswar SYAMSU; Surono SURONO; Nurika Asih SETIYANI; Selvia Mahilda RIDHOHA; Aninda Sekar ZAHRA; Nadia STEPANI; Muhammad Rasyid RAMADHAN
Menara Perkebunan Vol. 90 No. 2 (2022): 90 (2), 2022
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v90i2.502

Abstrak Bakteri mampu menghasilkan siderofor umtuk mengkelat besi dalam lingkungan dan digunakan oleh tanaman sebagai kofaktor dalam pembentukan klorofil untuk pembentukan glukosa. Penelitian ini bertujuan untuk mendapatkan bakteri penghasil siderofor tinggi dari akar nanas Simadu, Kabupaten Subang Provinsi Jawa Barat. Bakteri diisolasi dari akar nanas Simadu, kemudian diseleksi dengan membedakan morfologinya dan diidentifikasi sebagai penghasil siderofor pada media Chrome Azurol S (CAS). Diperoleh 10 isolat bakteri (M1 sampai dengan M10) yang mampu menghasilkan siderofor. Isolat bakteri M7 memiliki kemampuan menghasilkan siderofor tertinggi. Isolat M7 teridentifikasi sebagai bakteri Gram negatif. Hasil analisis pohon filogenetik berdasarkan sekuensing 16S rDNA menunjukkan isolat kelompok genus Providencia. Dibandingkan dengan Providencia vermicola , isolat standar dari InaCC yang berasal dari akar Curcuma zedoaria, M7 menunjukkan produksi siderofor yang lebih tinggi pada media LB pada kondisi aerobik.[Kata kunci: Isolasi bakteri, Providencia, akumulasi siderofor, akar nanas Simadu Subang]Abstract Bacteria can produce siderophores for chelating iron in environments and are used by plants as an ingredient cofactor in building chlorophyll for glucose production. This study aims to obtain high siderophore-producing bacteria from the roots of pineapple Simadu, Subang District, West Java Province. Bacteria were isolated from Simadu pineapple roots, then selected by distinguishing their morphology and identified as producing siderophores with Chrome Azurol S (CAS) media. Obtained 10 bacterial isolates (M1 to M10) capable of producing siderophores. Bacterial isolate M7 had the highest siderophore production ability. M7 isolate was identified as Gram-negative bacteria. The results of the phylogenetic tree analysis based on 16S rDNA sequencing showed this isolate as the genus Providencia. Compared to the Providencia vermicola as reference isolate from InaCC derived from the roots of Curcuma zedoaria, M7 showed higher siderophore production in LB media under aerobic conditions.[Keywords: Bacteria isolation, Providencia, siderophore accumulation, Simadu Subang pineapple root]

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