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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Majalah Kedokteran Bandung Agrointek Menara Perkebunan
Prayoga SURYADARMA
Department Of Agricultural Industrial Technology, Faculty Of Agricultural Technology, Bogor Agricultural Institute, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Medicine & Pharmacology

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Glycerol Utilization as Substrate for Ethanol Production in Escherichia coli Recombinant under an Aerobic Condition Wahyu Suradi Pranata; Prayoga Suryadarma; Djumali Mangunwidjaja
Journal of Tropical Life Science Vol. 8 No. 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.14

Abstract

The utilization of glycerol as a substrate for ethanol production in E. coli recombinant harboring ethanologenic gens (PDC and ADHB) under aerobic conditions was investigated. This research was conducted by using E. coli BW25113 which compared the growth characteristics on glycerol and glucose as carbon source. E. coli can grow well on both substrates and it consumed glycerol faster than glucose. On glucose, E. coli occurred overflow metabolism that indicated by high acetate accumulation. Meanwhile, on substrate of glycerol the acetate accumulation could be reduced. Using glycerol substrate on E. coli increased piruvate accumulation, comparing with glucose. Therefore, the growth characteristic of glycerol was more effective. In E. coli ∆pta/pHfdh/pTadhB-pdc utilized glycerol substrate was able to accumulate pyruvate as intermediate metabolite for produce 2.18 gL-1 ethanol.
Co-Authors Ahmad Faried Ani Suryani Aninda Sekar ZAHRA Djumali Mangunwidjaja Djumali Mangunwidjaja Firman Fuad Wirakusumah Hanifah FUADI Khaswar Syamsu Muhammad Rasyid RAMADHAN Murtiningrum Murtiningrum Nadia STEPANI NISA RACHMANIA MUBARIK Nuraliah Rusman Nurika Asih SETIYANI Pranata, Wahyu Suradi Rachmawati Noverina Sarah Sumayyah Selvia Mahilda RIDHOHA Surono SURONO Wireni Ayuningtyas