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All Journal Jurnal Fisioterapi dan Ilmu Kesehatan Sisthana (JUFDIKES) Jurnal Sains dan Kesehatan
Ira P. Ely
Sekolah Tinggi Ilmu Kesehatan Maluku Husada
Health Professions Public Health

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Skrining Fitokimia Dan Uji Aktivitas Ekstrak Etanol Biji Labu Kuning (Curcubita Moschata) Terhadap Daya Hambat Bakteri Staphylococcus Aureus Ira P. Ely; Aulia Debby Pelu; Lukman La Bassy
JURNAL FISIOTERAPI DAN ILMU KESEHATAN SISTHANA Vol. 2 No. 2 (2020): Juli : Jurnal Fisioterapi dan Ilmu Kesehatan Sisthana
Publisher : Stikes Kesdam IV/Diponegoro Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jufdikes.v2i2.157

Abstract

Pumpkin (Cucurbita moschata) is an important vegetable because of its nutritional value and health benefits. This plant is a rich source of carotenoids, which are rich in water-soluble vitamins, phenolics, flavonoid polysaccharides, mineral salts, and vitamins, all of which are beneficial for health. Pumpkin fruit has a very thick and hard skin, so it can act as a barrier to the rate of respiration, the release of water through the evaporation process, and the entry of air that causes the oxidation process. The purpose of this study was to determine the antibacterial activity of pumpkin seed extract (curcubita moschata). ) against the inhibition of staphylococcus aureus bacteria by well diffusion method. This type of research is a true experimental laboratory using the well diffusion method. The ethanol extract of pumpkin seeds (curcubita moschata) which was tested using the well method was then divided into several concentrations including 10%, 15%, 20%, and 25%. On the NA media that has been made then smeared with Staphylococcus aureus bacteria which had been previously sterilized, then four wells were made in a petri dish after which pumpkin seed extract was added to each concentration. Chloramphenicol was used as a positive control as a positive control. It was then incubated at 37o for 24 hours, then the inhibition zone formed was then measured. The result of this research is pumpkin seed extract (curcubita moschata) has antibacterial activity in the presence of inhibition around the wells of each extract concentration. At a concentration of 10% extract with an inhibitory diameter of 19 mm, a concentration of 15% in an inhibitory diameter of 20 mm, a concentration of 20% in an inhibitory diameter of 21 mm and for a concentration of 15% with an inhibitory diameter of 24 mm, it can be concluded that the extract of pumpkin seeds (curcubita) moschata.) has antibacterial activity against Staphylococcus aureus.
SKRINING FITOKIMIA DAN UJI AKTIVITAS EKSTRAK ETANOL BIJI LABU KUNING (Curcubita Moschata) TERHADAP DAYA HAMBAT BAKTERI Staphylococcus aureus Aulia Debby Pelu; Ira P. Ely; Lukman La Bassy
Jurnal Sains dan Kesehatan Vol. 4 No. 1 (2020): Juni : Jurnal Sains dan Kesehatan (JUSIKA)
Publisher : Universitas Muhamadiyah Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (896.518 KB) | DOI: 10.57214/jusika.v4i1.151

Abstract

Labu kuning (Cucurbita moschata) merupakan sayuran penting karena nilai nutrisinya dan manfaat kesehatannya. Tanaman ini adalah sumber karotenoid yang kaya akan vitamin larut air, fenolat, flavonoid polisakarida, garam mineral, dan vitamin yang semuanya bermanfaat bagi kesehatan. Buah labu kuning mempunyai kulit yang sangat tebal dan keras, sehingga dapat bertindak sebagai penghalang laju respirasi, keluarnya air melalui proses penguapan, maupun masuknya udara penyebab proses oksidasi.. Tujuan dari pada penelitian ini adalah untuk mengetahui aktivitas antibakteri ekstrak biji labu kuning (curcubita moschata) terhadap daya hambat bakteri staphylococcus aureus dengan metode Difusi sumuran. Jenis penelitian ini adalah True Eksperimental laboratorium dengan menggunakan metode difusi sumuran. Sebagai control positif yang di ganakan adalah kloramfenikol. Selanjutnya diinkubasi pada temperatur 37o selama 24 jam yang kemudian zona hambat yang terbentuk selanjutnya diukur. Hasil dari penelitian ini adalah ekstrak biji labu kuning (curcubita moschata) memiliki aktivitas antibakteri dengan adanya daya hambat disekitar sumuran dari masing-masing konsentrasi ekstrak. Pada konsentrasi ekstrak 10% diameter daya hambat 19 mm, konsentrasi 15% diameter daya hambat 20 mm, konsentrasi 20% diameter daya hambat 21 mm dan untuk konsentrasi 15% dengan diameter daya hambat 24 mm, sehingga dapat disimpulkan bahwa ekstrak biji labu kuning (curcubita moschata.) memiliki aktivitas antibakteri terhadap bakteri staphylococcus aureus.
Co-Authors Aulia Debby Pelu Aulia Debby Pelu Lukman La Bassy