Syarifah Wahyuni
Bagian Kimia, Jurusan Teknologi Laboratorium Medis, Politeknik Kesehatan Kemenkes Aceh, Aceh

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Penggunaan ubi kayu putih sebagai media alternatif kultur Candida albicans Safridha Kemala Putri; Syarifah Wahyuni; Farah Fajarna
Jurnal SAGO Gizi dan Kesehatan Vol 4, No 1 (2022): Juli - Desember
Publisher : Politeknik Kesehatan Kemenkes Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/gikes.v4i1.1053

Abstract

Background: Candida albicans is a fungus that requires organic compounds as a source of carbon and energy for growth and metabolic processes obtained from carbohydrates. One of the alternative media from materials that are readily available in nature but have not been widely utilized, namely from tubers such as white cassava (Manihot esculenta C.), which is believed to have a high carbohydrate content according to the needs of Candida albicans.Objectives: The study aims to determine whether white cassava can be used as an alternative medium for Candida albicans culture.Methods: The method used in this study is experimental, namely by seeing whether Candida albicans can grow or not on alternative media of white cassava. This study begins with the collection of Candida albicans specimens in patients with candidiasis, and then research is carried out at the Microbiology Laboratory of the Medical Laboratory Technology Department of the Aceh Ministry of Health Polytechnic. This research was carried out in 2020. The sample of this study was white cassava meat, as much as 150 grams. This study collected data by culturing Candida albicans on alternative media with raw materials for white cassava meat. For the identification of Candida albicans species, confirmation tests were carried out using the Germ tube test method. Furthermore, the data obtained from the assessment of colony growth (+) positive Candida albicans on white cassava media (Manihot esculenta C.) incubated for 72 hours (± three days) at 370C.Results: Based on the macroscopic and microscopic assessments, Candida albicans can grow well on white cassava media (Manihot esculenta C.).Conclusion: Alternative media of white cassava raw materials (Manihot esculenta C.) can be used as a culture medium for Candida albicans.
Efektivitas pertumbuhan Candida albicans pada media alternatif air rebusan kacang kedelai (Glycine max (l) Merr) Rahmayanti Rahmayanti; Siti Hadijah; Syarifah Wahyuni; Safwan Safwan
Jurnal SAGO Gizi dan Kesehatan Vol 4, No 1 (2022): Juli - Desember
Publisher : Politeknik Kesehatan Kemenkes Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/gikes.v4i1.1067

Abstract

Background: Candida albicans are one of the pathogenic fungi in humans whose growth requires carbohydrates and proteins to grow and develop. Soybeans have a reasonably good nutritional content and potential as an alternative medium for the growth of Candida albicans fungi, and soybeans are very easy to find in the community, so they can be used as an alternative medium to replace SGA (Sabouraud Glucose Agar). Soybeans (Glycine max (L.) Merr) have nutritional content consisting of oils, carbohydrates, and minerals that allow them to source nutrients and food for fungi. Furthermore, one of them that can be utilized from soybeans is cooking water.Objectives: The study aims to determine whether soybean (Glycine max (L.) Merr) cooking water media can be an effective alternative for Candida albicans culture.Methods: An analytical study using an experimental design was conducted in the Microbiology laboratory at the Department of Medical Laboratory Technology, Health Polytechnic of Aceh, Ministry of Health, in September 2022. The sample used in this study was soybean cooking water as an alternative medium for Candida albicans. Data collection is based on the culture (breeding) of Candida albicans on alternative media with soybean decoction water as raw material. For the identification of Candida albicans species, confirmation tests were carried out using the germ tube test method. Then the growth assessment of positive (+) colonies of Candida albicans on alternative media of soybean cooking water was incubated for 72 hours (± three days) at 37°C. Data analysis was only done descriptivelyResults: There is the growth of Candida albicans fungal colonies on alternative media from soybean cooking water with macroscopic characteristics of yellowish white color, yeast smell, smooth, smooth surface, flat edges, small colonies, and many colonies. On microscopic observation, Candida albicans fungus was found with microscopic characteristics of round, oval, small, thin-walled, yeast-like cells and pseudohyphae.Conclusion: Soybean (Glycine max (L) Merr) cooking water media is effectively used as an alternative media for the growth of Candida albicans.