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All Journal Journal of Indonesian Medical Laboratory and Science (JoIMedLabS)
Iqlila Romaidha
D-III Analis Kesehatan, STIKes Borneo Cendekia Medika, Kalimantan Tengah, Indonesia
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology

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ANALISA EKSTRAK ETANOL DAUN BAJAKAH KAIT-KAIT (Uncaria acida (Hunt.) Roxb.) TERHADAP BAKTERI Escherichia coli MENGGUNAKAN METODE KIRBY BAUER Mia Irawan; Febri Nur Ngazizah; Riky Riky; Iqlila Romaidha
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.49

Abstract

Kalimantan forest is the largest tropical rain forest which is one of the places with biodiversity that has the potential for development in the field of health or medicine, one of the plants used for treatment is hook root (Uncaria acida (Hunt.) Roxb.). This plant has indole alkaloid compounds, triterpenes, flavonoids and phenylpropanoids that act as antibacterial. In this study, an antibacterial test was conducted using the Kirby Bauer method to determine the antibacterial activity which was characterized by the formation of an inhibition zone around the paper disc. The bacteria tested were E. coli. The extract tested in this study was the ethanolic extract of U. acida leaves with 5 concentrations of treatment (5 mg/mL, 15 mg/mL, 30 mg/mL, 40 mg/mL and 50 mg/mL). The average result of the inhibition zone of the ethanolic extract of U. acida leaves in a concentration of 5 mg/mL is 8.1 mm in the weak category, the concentration of 15 mg/mL is 8.3 mm in the weak category, the concentration of 30 mg/mL is 9.1 mm in the weak category, 40 mg/mL ie 9.2 mm in the weak category and 50 mg/mL in the moderate category. Using significance (? > 0.05) which means that the inhibition zone data on the growth test of E. coli bacteria at the 5 concentrations affected the inhibition zone of E. coli bacteria.
PERBANDINGAN EFEKTIVITAS EKSTRAK BAWANG PUTIH (Allium sativum L.) DENGAN DAUN BAJAKAH (Uncaria acida Roxb.) SEBAGAI LARVASIDA VEKTOR DENGUE Aedes aegypti Fitra Akbar Mulyono; Iqlila Romaidha; Nur Aini Hidayah Khasanah; Febri Nur Ngazizah
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.50

Abstract

Dengue fever is a disease caused by the dengue virus carried by the A. aegypti mosquito. Making natural larvicides from plants such as Alium sativum L. and Uncaria acida Roxb. are indispensable. This study aims to make natural larvicides from A. sativum and U. acida. This study is an experimental study using a Completely Randomized Design (CRD) to see the effectiveness of the extract A. sativum with U. acida as larvicide of the mosquito A. aegypti.This study used 20 mosquito larvae for each concentration and repeated 4 times. The results showed that the larvicide of A. sativum at the highest concentration reached 100% mortality, while the larvicide of U. acida at the highest concentration reaching 80% mosquito larvae death A. aegypti. Analysis of research data was processed using SPSS version 20. One Way Anova analysis of A. sativum extract was sig 0.049 and U. acida extract was sig 0.245. In the extract of A. sativum there was a significant difference in the mortality of mosquito larvae at each concentration, while in the extract of U. acida there was no significant difference in the mortality of mosquito larvae at each concentration. T-test analysis of the extracts of A. sativum and U. acida was sig 0.80, so there was no difference in effectiveness between the two larvicidal extracts. It can be concluded that the larvicidal research results of A. sativum extract and U. acida extract were equally effective on A. aegypti larvae.
Co-Authors Febri Nur Ngazizah Fitra Akbar Mulyono Mia Irawan Nur Aini Hidayah Khasanah Riky Riky