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All Journal STOMATOGNATIC- Jurnal Kedokteran Gigi Journal of Indonesian Dental Association
Ariyati Retno Pratiwi
Departemen Biologi Oral, Fakultas Kedokteran Gigi, Universitas Brawijaya
Dentistry Medicine & Pharmacology

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Hubungan Ekspresi Osteocalcin dan Interleukin-1β dengan Status Inflamasi Periodontal Rahmavidyanti Priyanto; Malianawati Fauzia; Ariyati Retno Pratiwi; Neny Roeswahjuni; Novi Khila Firani
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 19 No 2 (2022)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v19i2.34736

Abstract

Periodontitis is inflammation of the periodontal tissue that can cause progressive damage to the periodontal ligament and alveolar bone. Clinical examination to determine the degree of damage to the alveolar bone using a periodontal probe instrument or probing. Cellular activity in the gingival crevicular fluid is thought to be a biomarker to determine the inflammatory status of the periodontal tissue. Cellular immune responses suggest the role of cytokines in the pathogenesis of gingivitis and periodontitis. The cytokines observed in this study were the protein Osteocalcin and Interleukin-1β (IL-1β) in the gingival crevicular fluid (GCF). In this study, 67 patients were examined for the condition of their periodontal tissues including the severity of gingivitis and periodontal probing. The gingivitis group was divided into 4 groups, namely normal, mild, moderate, and severe. Meanwhile, for probing samples were grouped based on probing into 3 groups. Group I (0-3 mm), group II (3.1-5 mm) and group III (> 5 mm). GCF was obtained using paper points that were left in the gingival sulcus for 30 seconds. Then paper points are put into cryovial tubes which are sent to the laboratory for ELISA test. The observed data on the expression of Osteocalcin and IL-1β were then performed statistical tests. Osteocalcin and IL-1β expression showed a significant difference with the severity of gingivitis cases. In the other hand, it did not show a significant difference with difference of periodontal pocket depth. Osteocalcin and IL-1β expression associated with the severity of gingivitis cases.
The Antibacterial Activity of Chitosan from Haruan (Channa striata) Fish Scales on the Growth of Streptococcus sanguinis Wijayanti Diah Wasi Hutami; Deby Kania Tri Putri; Amy Nindia Carabelly; Indah Listiana Kriswandini; Ariyati Retno Pratiwi; Muhammad Luthfi
Journal of Indonesian Dental Association Vol. 3 No. 2 (2020): October
Publisher : Indonesian Dental Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jida.v3i2.450

Abstract

Introduction: Recurrent Aphthous Stomatitis (RAS) is the most common oral lesion in Banjarmasin, with a prevalence of 45.42%. Streptococcus sanguinis (S. sanguinis) is thought to be one of the factors causing RAS. One natural ingredient that is often used by the people of South Kalimantan is the Haruan fish. The high Haruan fish consumption will eventually leave fish scales that have the potential to pollute the environment if not addressed immediately. Fish scales contain chitin, which when deacetylated produces chitosan, which has antibacterial properties. Objectives: The purpose of this study was to determine the effectiveness of the chitosan from the scales of the Haruan fish (Channa striata) at inhibiting S. sanguinis growth. Methods: This study used a randomized pretest-posttest with control group design using five treatments. The five treatments were subjected to liquid dilution using the UV-Vis Spectrophotometer method to obtain minimum inhibitory concentration (MIC) and a solid dilution test using the Total Plate Count method to obtain minimum bactericidal concentration (MBC). Results: The results showed that Haruan scale chitosan proved to be effective as an antibacterial against S. sanguinis, with a MIC of 1.25% and a minimum lethal concentration of 2.5%. One-Way Anova test results showed significance for the MIC test (p = 0,000) and MBC test (p = 0.000; p < 0.05). Conclusion: Chitosan from Haruan fish scales is both inhibitory and lethal to S. sanguinis.
Co-Authors Amy Nindia Carabelly Indah Listiana Kriswandini Malianawati Fauzia Muhammad Luthfi Neny Roeswahjuni Novi Khila Firani Rahmavidyanti Priyanto Tri Putri, Deby Kania Wijayanti Diah Wasi Hutami