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All Journal Jurnal Sain Veteriner Buletin Veteriner Udayana
Juli Melia
Faculty Of Veterinary Medicine Universitas Syiah Kuala, Banda Aceh
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Pemanfaatan Limbah Kulit Buah Salak sebagai Antibakteri Pseudomonas aeruginosa Amalia Sutriana; Ananda Putra Perdana; Budianto Panjaitan; Juli Melia; Mulyadi Adam
Buletin Veteriner Udayana Vol. 14 No. 6 December 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i06.p19

Abstract

Pseudomonas aeruginosa is an opportunistic pathogenic bacteria that can infect both human and animal. This bacteria has undergone Multi Drug Resistance caused by irrational use of antibiotics. Therefore, it is necessary to conduct research to obtain antibacterial compounds from natural ingredients, one of them is from salak fruit peel (Salacca zalacca). This study aims to investigate the antibacterial activity of salak fruit peel extract in inhibiting the growth of P. aeruginosa in vitro. Salak fruit peel was extracted by maceration method using ethanol 96%. Concentrations of salak fruit peel extract used in this study were 5%, 10%, 20% and 30%. Disk containing chloramphenicol was used as positive control and aquadest was used as negative control. Antibacterial activity of salak fruit peel extract was conducted using Kirby-Bauer method. The data was analyzed descriptively. The results showed that there were no inhibition zone was formed surrounding the disc containing salak fruit peel extract at concentrations of 5%, 10%, and 20%. At concentration of 30%, the inhibition zone was formed with diameter 6.2 mm. It could be concluded that salak fruit extract at concentration less than 30% did not have antibacterial activity againts P. aeruginosa in vitro.
Pengaruh Pemberian Gonadotropin Releasing Hormone (GnRH) Terhadap Peningkatan Kualitas Spermatozoa Sapi Bali Pengaruh Pemberian Gonadotropin Releasing Hormone (GnRH) Terhadap Peningkatan Kualitas Spermatozoa Sapi Bali Amalia Sutriana; Tongku Nizwan Siregar; Feby Claudia Sirait; Juli Melia; Hafizuddin .
Jurnal Sain Veteriner Vol 40, No 3 (2022): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.65067

Abstract

Penelitian ini bertujuan mengetahui pengaruh pemberian gonadotropin releasing hormone (GnRH) terhadap peningkatan kualitas spermatozoa sapi bali. Penelitian ini menggunakan dua ekor sapi bali berumur 2 dan 4 tahun dengan rancangan pola bujursangkar latin 2 perlakuan dan 2 periode perlakuan. Perlakuan yang dilakukan adalah P1 (NaCl fisiologis) dan P2 (GnRH 100 µg) dengan interval periode waktu perlakuan adalah satu minggu. Sampel semen dikoleksi menggunakan vagina buatan tiga hari setelah perlakuan. Data hasil pemeriksaan kualitas spermatozoa sapi bali dianalisis dengan uji Wilcoxon Signed Ranks Test. Berdasarkan uji statistik menunjukkan bahwa pemberian GnRH 100 µg tidak berpengaruh nyata (P>0,05) terhadap pemeriksaan warna, pH, konsistensi, volume, konsentrasi dan motilitas spermatozoa sapi bali. Hasil pemeriksaan menunjukkan bahwa warna semen pada kedua perlakuan adalah krem dan putih susu. Derajat keasaman (pH); konsistensi; volume semen (ml); konsentrasi (10⁶/ml); dan motilitas spermatozoa (%) sapi  bali pada P1 vs P2 adalah 7,0±0,0 vs 7,0±0,0; 2,5±0,71 vs 3,0±0,0; 4,5±2,8 vs 4,8±2,8; 925±106,07 vs 1210±155,56; dan 69,0±1,41 vs 71,5±0,71 (P>0,05). Disimpulkan bahwa pemberian GnRH 100 µg tidak memengaruhi kualitas spermatozoa sapi bali.
Co-Authors Amalia Sutriana Amalia Sutriana Ananda Putra Perdana Budianto Panjaitan Feby Claudia Sirait Hafizuddin Hafizuddin Mulyadi Adam Tongku Nizwan Siregar