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All Journal e-Journal BUDIDAYA PERAIRAN JURNAL ILMIAH PLATAX
Diane J. Kusen
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Environmental Science

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Journal : e-Journal BUDIDAYA PERAIRAN

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Uji fitokimia dan aktivitas antimikroba ekstrak kulit pisang kepok (Musa paradisiaca) terhadap bakteri Aeromonas hydrophila Aylifia S. Somba; Reni L. Kreckhoff; Diane J. Kusen; Henky Manoppo; Reiny A. Tumbol; Fitje Losung
e-Journal BUDIDAYA PERAIRAN Vol. 11 No. 1 (2023)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.11.1.2023.42192

Abstract

This research was conducted to qualitatively analyze the phytochemical content of kepok banana (Musa paradisiaca) peel extract and the effectivity of the extract as antibacterial against Aeromonas hydrophila. Fresh yellow color banana peels were washed, drained, then cut into pieces, crushed and dried at room temperature. Extraction was carried out by maceration method with 70% and 95% ethanol as solvent. A total of 90 g was put in an elemeyer glass then added with solvent until all the banana peels were submerged. The immersion time was 3x24 hours at room temperature while stirring occasionally so that the extraction occurs properly. Furthermore, the extract was filtered with Whatman paper no. 42 to separate the residue from the filtrate. The filtrate was then concentrated using a rotary evaporator. Phytochemical examined consisted of alkaloid, flavonoid, sternoid, triterpenoid, saponin, and tannin. Inhibition test against A. hydrophila was carried out by disc diffusion method using 5 different extract concentrations as treatments, namely A: 0%, B:10%, C:20%, D:30%, E:40%, F:50%. In the inhibition test, the paper disc was dipped in each treatment and then placed on agar media previously stocked with bacteria. The inhibitory zone was measured based on the clear area formed around the disc and observed at 24, 48, and 72 hours. Phytochemical test found that phenol and tannin were only detected in extracts with 95% ethanol while terpenoids and steroids were only found in  ethanol70%.  Flavonoid was detected in the extract using either ethanol70% and 95%. The results of the inhibitory test showed that the kepok banana peel extract could inhibit the growth of A. hydrophila bacteria after 48 hours of incubation. Inhibition zones formed at both 48 hours and 72 hours tended to increase in line with the increase in concentration even though the inhibitory power produced was categorized as "medium".
Limbah hasil produksi budidaya ikan sistem karamba jaring tancap di Desa Eris, Talikuran dan Kaima Kabupaten Minahasa Asmirawati Zai; Diane J. Kusen; Suzanne L. Undap; Novie P.L. Pangemanan
e-Journal BUDIDAYA PERAIRAN Vol. 11 No. 1 (2023)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.11.1.2023.43451

Abstract

This study aimed to obtain the amount of organic waste, nitrogen and phosphorus that wasted to  lake and measuring water quality parameters, temperature, dissolved oxygen (DO), acidity (pH) and dissolved solids (TDS) in the area of fixed net cages at Lake Tondano in the villages of Eris, Talikuran and Kaima. This research was carried out in Lake Tondano, Eris, Talikuran and Kaima villages . The time of the study was carried out from March to July 2022. This study used     a descriptive exploration method. Data obtained included primary and secondary data. Primary data otained from interview results and parameter measurements of the water quality in the field (in situ). Secondary data obtained through various resources such as journals and the results of previous studies related to the topic under study. The data of organic waste, nitrogen, and phosphorus were analyzed descriptively using Schmittou method. The results of analyzing the parameters quality of water was presented in the form of tables and graphs then compared with the class of water quality standards in Government Regulation Number 22, 2021 regarding the Implementation of Environmental Protection and Management. The results showed that the amount of organic waste in Eris Village was 874.74 tons/year, Talikuran village 170.52 tons/year, and kaima village 105.80 tons/year, the amount of nitrogen waste in Eris village is 49.86 tons/year, Talikuran village 9.67 tons/year, and kaima village 6.00 tons/year, the amount of phosphorus waste in Eris Village is 17.24 tons/year, Talikuran Village 3.35 tons/year and Kaima Village 2.08 tons/year. The temperature in Eris Village is in the range of 27.4-29.5 °C, Talikuran Village is 26.5-30.3 °C and   Kaima Village is 26.6-30 °C, DO in Eris Village is 5.25-6, 74 mg/L, Talikuran Village 3.76-6.69 mg/L and Kaima Village 4.86-7.18 mg/L, pH in Eris Village was in the range of 7.2-7.9, Talikuran Village 7.1-7.8 and Kaima Village 7,3-7,8, TDS in Eris Village is in the range of 0.104-0.114 g/L, Talikuran Village is 0.117-0.160 g/L and Kaima Village is 0.104-0.114 g/L. The measured water quality parameter data still meets the water quality standard value.
Co-Authors Asmirawati Zai Aylifia S. Somba Edwin L. A. Ngangi Febi S.D. Saragih Fitje Losung Henky Manoppo Henneke Pangkey Kreckhoff, Reni L. Mikraim J. Kaseger Nego E. Bataragoa Novie P.L. Pangemanan Reiny A. Tumbol Sammy N. J. Longdong Suzanne L. Undap Winda Mercedes Mingkid