Sammy N. J. Longdong
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Konsentrasi merkuri ikan capungan Cardinalfish (Sphaeramia orbicularis) di area budidaya perairan Pintu Kota Kecamatan Lembeh Utara, Bitung Sabono, Mateda M.; Undap, Suzanne L.; Longdong, Sammy N. J.
e-Journal BUDIDAYA PERAIRAN Vol 8, No 1 (2020)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.8.1.2020.27539

Abstract

The aims of this research were to determine the total mercury concentration of Cardinalfish (Sphaeramia Orbicularis) and the waters in aquaculture area of Pintu Kota, North Lembeh, Bitung, and its water quality paramaters (temperature, salinity, pH). The research was conducted from June to Nopember 2019 in the cultivation area of tiger grouper fish in which the fish and seawater were sampled and analyzed in the laboratory. Determination stations were done by purposive sampling which refers to the physiographic lacation wherever possible in order to represent or describe these waters. Station 1 represented the sea transpartion port of Lembeh Island (no cultivation), Station 2 where the cultivation A, Station 3 where the cultivation B. The results showed the concentration  of the mercury obtained at Station 1 ranged from 0.00756 -0.00789 ppm, Station 2 ranged 0.00394-0.00418 ppm, and Station 3 ranges from 0.00506-0.00564 ppm. Whereas the concentration of mercury in seawaters samples were detected at Station 1, 2, and 3 were < 0.003 ppm. The temperature ranged between 26.2 – 26.7°C, pH 7 and salinity 30-33 ppt. In general, the existence of the phisycal chemical parameters of water was in good measurement after comparing with the water quality standard of Kep. MENLH No. 51/2004. Mercury concentrations in all samples in Pintu Kota waters of North Lembeh as compared to WHO/FDA standard are still in good condition but have been already contaminated by mercury.
PKM Kelompok Pembudidaya Ikan Di Kelurahan Tendeki Kecamatan Matuari Kota Bitung Provinsi Sulawesi Utara Mudeng, Joppy D.; Longdong, Sammy N. J.
e-Journal BUDIDAYA PERAIRAN Vol 7, No 2 (2019)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.7.2.2019.25990

Abstract

"Gemusis" fish cultivation group is one of the aquaculture business groups that exist in Tendeki Village, Matuari District, Bitung City, North Sulawesi Province. The width of  pond owned is around 3 ha with an average production of 2 tons per year. The main problem in this group is the difficulty in obtaining superior seeds, expensive feed prices and often the fish that are kept stricken with disease, resulting in less production and even crop failure. Alternative solutions to problems in partner problems are accompanied by two approaches, namely the classical and individual approaches. The classical approach is carried out when delivering the material. An individual approach is taken at the time of practice. Lectures in class in the form of delivery of how to make tilapia seed with sex reversal methods for the maintenance of monosex, making low-quality quality feed made from local raw materials and producing maggot as natural food, as well as how to increase fish immunity by giving immunostimulants to fish feed. Demonstration and practice with techniques for making cheap feed ingredients made from local raw materials. In order for the fish cultivation activities carried out by the partners according to the activity plan, monitoring and assistance will be carried out after the lecture and practice activities. The results obtained showed the group are able to cultivate fish with monosex system, produce feed using local raw materials, maggot cultivation as a natural feed, and can treat fish diseases using mmunostimulant.
Uji penggunaan pelepah pisang dan spon pada pengemasan bibit rumput laut Kappahycus alvarezii dalam mencegah ice-ice Datangmanis, Adieng A. R.; Kreckhoff, Reni L.; Longdong, Sammy N. J.
e-Journal BUDIDAYA PERAIRAN Vol 8, No 2 (2020)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.8.2.2020.29980

Abstract

This search aimed to determine the shelf life of seaweed seedlings that are packaged using banana and sponge fronds in Styrofoam, determine the best seaweed packaging media in preventing damage or disease when seedlings are planted. The study was conducted for 5 weeks, from January to February 2020. Observation of seaweed seedlings was conducted at the Laboratory of Fish Health, Environment and Toxicology, Faculty of Fisheries and Marine Sciences and planting tests were carried out in Arakan Village, Tatapaan District. South Minahasa Regency.The research method was carried out in the form of: packing Kappaphycus alvarezii seaweed, observing seaweed seeds and planting test. The experiment was designed according to a factorial completely randomized 3 x 3 design so that there were two factors tested in this experiment, namely the packaging medium factor and the shelf life. The packaging medium factor (A), consists of the level ie, (a1) control / without medium, (a2) banana fronds, (a3) sponge; while the shelf life factor (B), consists of three levels namely, (b1) 24 hours, (b2) 36 hours and (b3) 48 hours with three treatments and three replications so that the total number is 27 units of experiment with the number of seeds needed as much as 108 kg. The treatments taken are A: control, B: Packaging seaweed seeds with the addition of banana midrib, C: Packaging seaweed seeds with the addition of a sponge. The data obtained were analyzed ANOVA statistics for factorial RAL using the JMP (SAS-institute) statistical program. The results of the analysis of the variance showed that differences in treatment (the combination between the storage medium and the shelf life) had a very significant effect on differences in the texture and color of seaweed ("P" <0.0001). However, the effect of the storage medium factor on the texture of seaweed did not change significantly at the time of the change in the shelf life factor level ("P" = 0.1664).
Efisiensi usaha akuakultur sistem mina padi kolam dalam di Kabupaten Minahasa Tenggara Toar, Debby; Mudeng, Joppy D.; Watung, Juliaan Ch.; Longdong, Sammy N. J.; Kusen, Diane J.; Wantasen, Adnan S.
e-Journal BUDIDAYA PERAIRAN Vol 9, No 2 (2021)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.9.2.2021.34845

Abstract

This study aimed to evaluate the efficiency of the aquaculture system of the deep water rice field aquaculture system in Southeast Minahasa Regency. The method used was survey method with the respondents were all cultivators who carry out the deep water rice field aquaculture system. The data collected included primary and secondary data, with data collection techniques of observation, interviews and literature study. Data analysis was done by combining qualitative and quantitative analysis. Quantitative data analysis carried out were revenue, income and efficiency and business feasibility. The results showed that the production of deep water rice field aquaculture system in Southeast Minahasa Regency for fish was 24,700 kg and rice 69,300 kg. The cost incurred was Rp. 499,392,000, - with a net profit of Rp. 487,707,998,-. The efficiency of deep water rice field aquaculture system in Southeast Minahasa Regency was 1.90 which means this business was said to be efficient, while the business feasibility value was 0.90 which means to continue deep water rice field aquaculture system business was needed to be considered
¬Kajian kelayakan lokasi budidaya sidat (Anguilla spp.) di Perairan Danau Tondano, Provinsi Sulawesi Utara Parenta, Villy D.; Pangemanan, Novie P. L.; Longdong, Sammy N. J.; Kusen, Diane J.; Salindeho, Indra R. N.; Tumembouw, Sipriana S.
e-Journal BUDIDAYA PERAIRAN Vol 9, No 2 (2021)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.9.2.2021.34850

Abstract

The objective of this research was to determine feasibility ility on eel fish (Anguilla spp.) culture locations in Tondano Lake, North Sulawesi Province in terms of water quality, both physical and chemical parameters of the waters. Determination of the sampling point is placed at three predetermined points, station I located at Eris Village, Station 2 located at Paleloan Village and Station 3 located at Paslaten Village. The results showed that the waters of Lake Tondano have three classes of suitability for the location of eel (Anguilla spp.) culture, that is very suitable (S1), suitable (S2), and not suitable. Station I of Eris Village included in the suitable class (S2), Station II at Paleloan Village is included in the very appropriate very suitable class (S1), while Station III at Paslaten Village included in the class that is not suitable to be used as a location for eel culture in terms of water quality
Pemberian minyak pala (Myristica fragrans) Houttuyn 1774 sebagai bahan anestesi dengan konsentrasi berbeda pada benih lobster air tawar (Cherax quadricarinatus) Von Martens 1868 Mamuko, Nelsi; Mingkid, Winda M.; Watung, Juliaan Ch.; Kreckhoff, Reni L.; Longdong, Sammy N. J.; Manginsela, Fransine B.
e-Journal BUDIDAYA PERAIRAN Vol 10, No 1 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.1.2022.35535

Abstract

This study aimed to examine the best concentration of nutmeg oil for anesthesia of freshwater crayfish (Cherax quadricarinatus) seeds and analyze the length of time to faint, the length of time to faint and the length of time to wake up for the seeds of freshwater crayfish (C. quadricarinatus) . This research was carried out at the Aquaculture Technology Laboratory, Faculty of Fisheries and Marine Sciences, Sam Ratulangi University, Manado. The time of the research was carried out from April 18, 2021 - May 18, 2021. This study used a completely randomized design (CRD) consisting of 5 treatments and 3 replications, thus there were 15 experimental units. Treatment A with a dose of nutmeg oil (0.7 mL), treatment B (1 ml), treatment C (1.3 mL), treatment D (1.6 mL) and treatment E (1.9 mL). Parameters observed during behavioral research, length of time to faint, length of time to faint and length of time to wake up. The results of the study using the ANOVA statistical analysis test and the BNT follow-up test (Least Significant Difference) showed that different doses of nutmeg oil had a very significant effect on the length of time to faint, the length of time to faint and the length of time to wake up. The length of time to pass out the fastest, the longest length of time unconscious and conscious longest duration was obtained in treatment E (1.9 mL).
Pertumbuhan dan kelangsungan hidup ikan nila Oreochromis niloticus salin pada padat penebaran yang berbeda Baring, Vira; Longdong, Sammy N. J.; Ngangi, Edwin L. A.; Sinjal, Hengky J.; Kalesaran, Ockstan J.; Paruntu, Carolus P.
e-Journal BUDIDAYA PERAIRAN Vol 10, No 1 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.1.2022.35757

Abstract

This study aimed to determine the effect stocking density on growth and determine the effect on the survival of saline tilapia. The experiment was conducted at the Aquaculture Technology Laboratory and the time of the study was from February to August 2021. The experiment was designed according to a completely randomized design (CRD) with 4 treatments and 3 replications so that there were 12 experimental units. The factors tested were differences in stocking density of tilapia with treatment A: 10 fish/20 liters, treatment B: 15 fish/20 liters, treatment C: 20 fish/20 liters, and treatment D: 25 fish/20 liters. Growth data and survival rate (%) were analyzed using analysis of variance for RAL, then LSD test (Least Significance Different) was performed. Observations of water quality include temperature, DO, pH, and salinity. The result of the analysis of variance showed that the difference in stocking density had a significant effect on growth, but on the survival rate had an insignificant effect. The results of the BNT test on growth showed that treatment A was not significantly different from treatments B and C, but treatments A, B, and C were significantly different from treatment D. The temperature range during the experimental period was between 29 0C – 30.9 0C, DO ranged from 4.07 ppm – 6.80 ppm, pH between 6.78 – 7.95, and salinity 25 ppt
Pertumbuhan Trepang Holothuria scabra yang dipelihara dalam kurungan dengan substrat berbeda di Perairan Pantai Bahoi Kudato, Vania E.; Sambali, Hariyani; Ngangi, Edwin L. A.; Kusen, Diane J.; Longdong, Sammy N. J.; Rimper, Joice R. T. S. L.
e-Journal BUDIDAYA PERAIRAN Vol 10, No 2 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.2.2022.36088

Abstract

This research was conducted to examine the effect of different substrates on the growth of Sea Cucumber Holothuria scabra reared in cages in Bahoi Coastal waters. This research was conducted from April to June 2021 in Bahoi Coastal Waters, West Likupang, North Minahasa. The method used in this research is the observation method with three treatments: A (sand), B (mud), C (seagrass), and three replications. The cages are made of nets size 100 cm x 100 cm x 50 cm as many as 9 cages. The sea cucumber size data was collected every two weeks. Samples of sea cucumber were taken randomly from each cage, left for ± 10 minutes, photographed, weighed, then recorded. Data collection for water quality is carried out every week during the day at the lowest tide, between 12:00 to 14:00. Water quality parameters were measured using a thermometer, refractometer, and litmus paper. Based on the research results, the average absolute weight growth and daily growth rate of sea cucumber reared with mud substrate showed the best results, that is 0.55 g and 0.72%, while the sand and seagrass substrates showed negative values. The results of ANOVA (analysis of variance) showed that there was no significant difference between absolute growth and daily growth rate of Sea Cucumber H. scabra reared on sand, mud, and seagrass substrates.
The Inhibitory Power Extract Of Balsamica Plant (Impatiens balsamica L) on Aeromonas hydrophila Bacteria Growth Galih Arif Kusuma; Sammy N. J. Longdong; Reiny A. Tumbol
Jurnal Ilmiah PLATAX Vol. 2 No. 2 (2014): EDISI MEI - AGUSTUS 2014
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.2.2.2014.7147

Abstract

This research aimed to assess the potential use of extracts of leaves, flowers, and stems of the balsamica plant increase inhibitory effect on the growth of Aeromonas hydrophila. The content of the plant contains a compound balsamica naphthoquinone, coumarin derivatives, tannins, flavonoids, and steroids. The active compounds have the ability as an antimicrobial. A hidrophyla bacteria, including opportunistic pathogens are almost always found in water and often cause disease when the fish in adverse conditions. Antibacterial activity test in this research using the spread plate method. Data obtained in the form of the extract, the results of bacterial inoculation, and the inhibition test results, will be analyzed, displayed with pictures and described descriptively. The result of the process of maceration extraction using ethanol 70% was obtained three extracts are concentrated leaf extract: 28.75 g, flower: 12.82 g, and rods: 29.48 g. The result of antibacterial extracts of leaves, stems, and flowers balsamica plant showed inhibitory activity on the A hydrophila bacteria that seems to be indicated by a clear zone around the paper disc. Based on the classification of leaf extract and extract of the stem can be classified in the class of strong, because the inhibition zone formed by the leaf extract is equal to 11.2 mm, and extract stem with 13.7 mm inhibition zone. Ability flower extract in inhibiting the growth of A hidrophila bacteria with 21.4 mm inhibition zone showed that the extract was included in a very strong class. Keywords: The ethanol extract balsamica, Impatiens balsamica L, Aeromonas hydrophila, antibacterial activity test.
Application of probiotic bacteria isolated from catfish (Clarias batrachus) intestine to enhance growth performance and resistance of carp (Cyprinus carpio) against Aeromonas hydrophila Febi S.D. Saragih; Henky Manoppo; Suzanne L. Undap; Edwin L. A. Ngangi; Sammy N. J. Longdong; Diane J. Kusen
Jurnal Ilmiah PLATAX Vol. 7 No. 2 (2019): ISSUE JULY - DECEMBER 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.2.2019.23724

Abstract

The objective of research was to isolate probiotic bacteria from catfish intestine and to examine its effects on the growth and resistance of carp against Aeromonas hydrophila.  The probiotic isolated from intestine was grown on MRS agar.  Fish used in this research was juvenile of carp with an average weight of 5.5 g obtained from Freshwater Aquaculture Board at Tatelu Village, North Sulawesi Province.  Before running the experiment, the juveniles were adapted for one weeks in 15 glass aquaria at a density of 15 individuals each.  After acclimatization, the fish were fed diet supplemented with probiotic at different concentrations namely 1x109 , 1x108 , 1x107,  1x106 cfu/mL for three weeks. The fish were fed two times a dayat 08.00 am and 17.00 pm with a dose of 5% of body weight per day. At the end of feeding, the fish were challenged with A. hydrophila.  Data collected included average growth rate, absolute growth, feed efficiency, and food conversion ratio.  Research results found that the addition of probiotic into feed was  able to increase growth and feed efficiency and reduce food conversion ratio as well (p<0.01). The best growth, feed efficiency and food conversion ratio were obtained in fish fed diet added with probiotic bacteria at 1x108 cfu/mL.  The highest survival rate was also observed in fish probiotic diet containing 1x108 cfu/mL.  As conclusion, probiotic bacteria isolated from catfish intestine was potential to improve growth, feed efficiency, reduce food conversion ratio and increase resistance of fish against bacterial pathogen.Keywords: probiotic, carp, Aeromonas hydrophila, feed efficiency, food conversion ratioABSTRAKPenelitian ini bertujuan untuk mengisolasi bakteri probiotik dari usus ikan lele dan menguji pengaruh pemberian probiotik dari usus ikan lele terhadap pertumbuhan dan resistensi ikan mas terhadap infeksi Aeromonas hydrophilla. Sumber probiotik diisolasi dari usus ikan lele menggunakan media MRS (de Man Rogosa Sharpe) dan bakteri Aeromonas hydrophilla ditumbuhkan pada media TSA (Tryptic Soy Agar). ikan uji diambil dari Balai Budidaya Air Tawar Tatelu , Provinsi Sulawesi Utara . ikan diaklimatisasi selama seminggu dalam 15 akuarium dengan kepadatan 15 ekor/akuarium dan berat awal rata-rata 5,5 g. Setelah diaklimatisasi ikan diberi pakan yang ditambahkan bakteri probiotik sebagai perlakuan dengan konsentrasi berbeda yaitu 1x109 , 1x108 , 1x107,  1x106 cfu/mL selama empat minggu sebanyak 5% /berat tubuh/hari dengan frekuensi pemberian pakan dua kali sehari yaitu jam 08.00 pagi dan jam 17.00 sore. Setelah diberi perlakuan maka diuji tantang dengan bakteri Aeromonas hydrophilla.  Data yang dikumpulkan terdiri dari laju pertumbuhan harian, pertumbuhan mutlak, efesiensi pakan, konversi pakan dan uji tantang terhadap bakteri patogen. Hasil penelitian mendapatkan bahwa penambahan bakteri probiotik dalam pakan mampu meningkatkan pertumbuhan, efesiensi pakan dan menurunkan konversi pakan (p<0.01). Pertumbuhan, efesiensi pakan dan konversi pakan yang terbaik dicapai pada ikan yang diberi pakan dengan penambahan bakteri probiotik 1x108 cfu/mL.  Kelangsungan hidup ikan mas yang diuji tantang dengan bakteri patogen yang paling tinggi dicapai pada ikan yang diberi pakan 1x108 cfu/mL. Sebagai kesimpulan bahwa bakteri probiotik yang diisolasi dari usus ikan lele berpotensi meningkatkan pertumbuhan, efesiensi pakan, menurunkan konversi pakan serta meningkatkan sistem kekebalan tubuh pada ikan mas.Kata Kunci : Probiotik, Ikan Mas, Aeromonas hydrophilla, Pertumbuhan, Efesiensi Pakan, Konversi Pakan