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Kejutan suhu pada penetasan telur dan sintasan hidup larva ikan lele (Clarias gariepinus) Aer, Christo V S; Mingkid, Winda M; Kalesaran, Ockstan J
e-Journal BUDIDAYA PERAIRAN Vol 3, No 2 (2015)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.3.2.2015.8322

Abstract

The aim of this study was determine the effect of temperature shock on hatching capability of the catfish eggs and its larval survivals.The study was conducted at the Board of Freshwater Aquaculture (BBAT ) in Tatelu  Dimembe District, North Minahasa Regency, North Sulawesi Province. Twelve tanks were set in this experiment. Two hundred (200) eggs were put in each tank with controlled temperature of 25 C, 27 C , 29 C , and 32 ºC. Each temperature was replicated 3 times. The experimental design used in data analysis was completely randomized design (CRD).  The results showed that the treatment temperature showed significant effect on  hatching of  eggs, while the difference in temperature had no effect on the survival rate of catfish larvae.   Keywords:  Temperature shock, eggs, hatching capability, survival rate,  larvae, Clarias gariepinus.
Kajian Kelayakan dan Pengembangan Lahan Budidaya Kepiting Bakau (Scylla spp) di Desa Kabupaten Minahasa Utara M, Mardiana; Mingkid, Winda; Sinjal, Hengky
e-Journal BUDIDAYA PERAIRAN Vol 3, No 1 (2015)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.3.1.2015.6951

Abstract

This research intended to evaluate the area suitability of  Scylla spp at Likupang II Village, North Minahasa. The research was done descriptively by observing water quality, area availability. SWOT analysis was used to determine the development strategy.   Data were collected by interview, observation and questionnaire.  Research result showed that the potential of Likupang II Village was suitable for Scylla spp culture.  SWOT analysis showed internal factors affected the development of Scylla spp culture.  The strength were location, water quality, government support; the weakness were seed availability, opportunity, government policy and treating were season and safety.   Keywords : Scylla spp, SWOT analysis, , Likupang II, area suitability
Penambahan Madu Dalam Pengenceran Sperma Untuk Motilitas Spermatozoa, Fertilisasi dan Daya Tetas Telur Ikan Nila (Oreochromis niloticus) Nainggolan, Ramses; Monijung, Revol D; Mingkid, Winda
e-Journal BUDIDAYA PERAIRAN Vol 3, No 1 (2015)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.3.1.2015.6948

Abstract

The research intends to determine the optimal concentration of honey addition in sperm dilution on sperm motility, fertilization and eggs hatchability of Nile Tolapia (Oreochromis niloticus). Experimental design used was a Complete Randomized Design (CRD) with three times repetition of four treatments. The concentration of honey addition used for treatment are A (0 ml of honey in 100 ml of physiological NaCl), B (0.60 ml of honey in 99.40 ml of physiological NaCl), C (0.65 ml of honey in 99.35 ml of physiological NaCl), D (0.70 ml of honey in 99.30 ml of physiological NaCl). Parent used consisted of five female parent and five male parent with 250 – 500 mg weight. Sperm dilution containers used was 12 plastic cups and the container fertilization and hatching eggs container used was 12 brass plates.  Spermatozoa motility observation performed immediately after dilution process. Fertilization observation was performed after 12 hours of fertilization process, and Egg Hatchability observation was performed after 60 hours of fertilization.  The observation results showed that the addition of honey in sperm dilution contributed significant effect on spermatozoa motility, fertilization and eggs hatchability of Nile Tolapia (Oreochromis niloticus). This observation showed that the addition of 0.70 ml of honey in a sperm dilution was the best treatment seen from the average percentage of spermatozoa motility (73,33 %), fertilization (80,00%) and eggs hatchability (77,33 %.) Keywords:       Honey, sperm dilution, spermatozoa motility, fertilization, eggs hatchability Nile Tolapia
Physical evaluation on freshwater crayfish, Cherax quadricarinatus, feed using several gluten materials Khartiono, Lady D.; Sampekalo, Julius; Mingkid, Winda M.
AQUATIC SCIENCE & MANAGEMENT Vol 2, No 1 (2014): April
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.2.1.2014.12410

Abstract

Title (Bahasa Indonesia): Uji fisik pakan lobster air tawar, Cherax quadricarinatus, yang menggunakan beberapa bahan perekat The objective of this study was to evaluate the physical parameters of feed for freshwater crayfish (Cherax quadricarinatus) using different glutens. This study used a completely randomized design with four treatments and three replications. Treatment A was feed with starch gluten, treatment B was feed with sago gluten, treatment C was feed with seaweed gluten and treatment D was a commercial feed (control). ANOVA showed that the treatments gave a significant effect (P <0.05) on the breaking rate in the water, sinking speed, homogeneity, and hardness. The results showed that feed using seaweed gluten is very good to use. Feed with starch gluten was also better than feed of sago gluten. However, for sinking speed, control diet and starch gluten-based feed were faster than that of seaweed gluten. The result for water quality analysis including temperature, pH, DO and ammonia during the study showed tolerable ranges for freshwater crayfish. Penelitian ini bertujuan untuk menguji secara fisik pakan lobster air tawar (Cherax quadricarinatus) yang menggunakan beberapa bahan perekat. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan empat perlakuan dan tiga ulangan. Perlakuan A pakan dengan bahan perekat kanji, perlakuan B pakan dengan bahan perekat sagu, perlakuan C pakan dengan bahan perekat rumput laut dan perlakuan D adalah pakan komersil (kontrol). Hasil analisis ragam menunjukkan bahwa antara perlakuan memberikan pengaruh nyata (P<0,05) terhadap kecepatan pecah pakan dalam air, dispersi pakan, kecepatan tenggelam, homogenitas, dan tingkat kekerasan. Pakan dengan bahan perekat rumput laut sangat baik untuk digunakan. Pakan dengan bahan perekat tepung kanji juga lebih baik dibandingkan pakan berbahan perekat sagu. Tetapi untuk kecepatan tenggelam pakan kontrol dan pakan berbahan perekat kanji lebih cepat dibandingkan pakan berbahan perekat rumput laut. Hasil analisis kualitas air meliputi suhu, pH, DO dan amonia selama penelitian menunjukkan masih dalam batas kisaran yang bisa ditoleransi lobster air tawar.
Kejutan suhu pada penetasan telur dan sintasan hidup larva ikan lele (Clarias gariepinus) Aer, Christo V.S; Mingkid, Winda M; Kalesaran, Ockstan J
e-Journal BUDIDAYA PERAIRAN Vol 3, No 2 (2015)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.3.2.2015.8325

Abstract

The aim of this study was determine the effect of temperature shock on hatching capability of the catfish eggs and its larval survivals.The study was conducted at the Board of Freshwater Aquaculture (BBAT ) in Tatelu  Dimembe District, North Minahasa Regency, North Sulawesi Province. Twelve tanks were set in this experiment. Two hundred (200) eggs were put in each tank with controlled temperature of 25 C, 27 C , 29 C , and 32 ºC. Each temperature was replicated 3 times. The experimental design used in data analysis was completely randomized design (CRD).  The results showed that the treatment temperature showed significant effect on  hatching of  eggs, while the difference in temperature had no effect on the survival rate of catfish larvae.   Keywords:  Temperature shock, eggs, hatching capability, survival rate,  larvae, Clarias gariepinus.
Variasi komposisi daun Ketapang dan kulit pisang sebagai media tumbuh pakan alami jentik nyamuk Tununu, Riky R.; Solang, Jhonly; Pangkey, Henneke; Mingkid, Winda M.; Kreckhoff, Reni L.; Kusen, Diane J.; Wantasen, Adnan
e-Journal BUDIDAYA PERAIRAN Vol 9, No 2 (2021)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.9.2.2021.33752

Abstract

This study aimed to 1) determine the best combination between ketapang and banana peels for the propagation of life feed mosquito larvae; 2) determine the culture media that can produce mosquito larvae optimally. The containers used in this study were 24 buckets with a size of 5 litres. Some of the materials used in the study were dried ketapang leaves, goroho banana peels, kepok banana peels and water. This study used a factorial Completely Randomized Design (CRD) consisting of two factors and three replications. The first factor was the difference in the number of ketapang leaves. The second factor was the difference in banana peels types. The first factor included the difference in the number of ketapang leaves, consisting of: Treatment 0 = without ketapang leaves, Treatment 1 = 50 grams of ketapang leaves, Treatment 2 = 75 grams of ketapang leaves, Treatment 3 = 100 grams of ketapang leaves. The second factor included differences in banana peels, which consist of: Treatment 1 = one kepok banana peel, Treatment 2 = one goroho banana peel. The results were different types of banana peels affected the numbers of mosquito larvae, where the best was kepok banana peels; the difference in weight of ketapang leaves did not have a significant effect on the number of mosquito larvae. The container used in this study was a bucket with a volume of 5 L. The banana peels used were goroho banana peels and ripe yellow kapok banana peels. Ketapang leaves were preapared with a weight 0f 0, 50, 75, and 100 grams, respectively. Shoe banana peels and  goroho banana, one skin eachand ketapang leaves 0, 50, 75, and 100 grams, respectively, put together wit the jalah used to wrap between ketapang leavesand spatu banana peels and goroho banana, then put into a container that has been filled wit 3 L of water.
Tingkat kelangsungan hidup juvenil lobster air tawar ‘red claw’ (Cherax quadricarinatus) dengan pemberian pakan alami berbeda Paputungan, Fikri; Mingkid, Winda M.; Sambali, Hariyani
e-Journal BUDIDAYA PERAIRAN Vol 9, No 1 (2021)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.9.1.2021.31063

Abstract

This study aimed to analyze the survival rate of freshwater crayfish Cherax quadricarinatus, using different natural feeds, namely Alona sp, Daphnia sp. and Artemia sp. Observing the growth of juvenile crayfish C. quadricarinatus. The study was conducted for two months (April - May 2019), at the Aquaculture Technology Laboratory. In this study, there were three treatments, namely different types of natural food. The treatments given were: Treat A Alona sp .; Treatment B Daphnia sp; and Treatment C Artemia sp. Feeding 25 individuals during the maintenance period is carried out twice a day, namely in the morning at 08.00 - 09.00 and 17.00 - 18.00 WIB. This study used a completely randomized design showing that the average value of SR (survival) in each treatment such as treatment A (Alona sp) was 60%, then treatment B (Daphnia sp) was 66.67% and finally treatment C ( Artemia sp) of 66.67%. Observation results for 21 days of mainten ance found an increase in length between 2 - 4 mm. The analysis of variance (ANOVA) showed that the differences in the types of natural food did not have a significant effect (P> 0.05) on the survival and growth of juvenile crayfish kept for 21 days
Pemberian minyak pala (Myristica fragrans) Houttuyn 1774 sebagai bahan anestesi dengan konsentrasi berbeda pada benih lobster air tawar (Cherax quadricarinatus) Von Martens 1868 Mamuko, Nelsi; Mingkid, Winda M.; Watung, Juliaan Ch.; Kreckhoff, Reni L.; Longdong, Sammy N. J.; Manginsela, Fransine B.
e-Journal BUDIDAYA PERAIRAN Vol 10, No 1 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.1.2022.35535

Abstract

This study aimed to examine the best concentration of nutmeg oil for anesthesia of freshwater crayfish (Cherax quadricarinatus) seeds and analyze the length of time to faint, the length of time to faint and the length of time to wake up for the seeds of freshwater crayfish (C. quadricarinatus) . This research was carried out at the Aquaculture Technology Laboratory, Faculty of Fisheries and Marine Sciences, Sam Ratulangi University, Manado. The time of the research was carried out from April 18, 2021 - May 18, 2021. This study used a completely randomized design (CRD) consisting of 5 treatments and 3 replications, thus there were 15 experimental units. Treatment A with a dose of nutmeg oil (0.7 mL), treatment B (1 ml), treatment C (1.3 mL), treatment D (1.6 mL) and treatment E (1.9 mL). Parameters observed during behavioral research, length of time to faint, length of time to faint and length of time to wake up. The results of the study using the ANOVA statistical analysis test and the BNT follow-up test (Least Significant Difference) showed that different doses of nutmeg oil had a very significant effect on the length of time to faint, the length of time to faint and the length of time to wake up. The length of time to pass out the fastest, the longest length of time unconscious and conscious longest duration was obtained in treatment E (1.9 mL).
Karakteristik morfometrik kerang mutiara Pinctada margaritifera di Perairan Arakan, Mantehage Bango dan Talengen Provinsi Sulawesi Utara untuk kelayakan budidaya Silalahi, Torang J. P.; Kalesaran, Ockstan J.; Lumenta, Cyska; Mingkid, Winda M.; Ngangi, Edwin L. A.
e-Journal BUDIDAYA PERAIRAN Vol 10, No 1 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.1.2022.35255

Abstract

The purpose of this study were (1) to determine the morphometric characteristic of pearl oyster Pinctada margaritifera in Arakan, Mantehage Bango, and Talengen waters of North Sulawesi Province, (2) to determine the ideal location for the feasibility of pearl oyster culture. Determination of the sampling location is done by purposive sampling. The shell morphometric dimensions measured were shell length, shell height, hinge length, shell thickness and shell weight. The measured water quality were temperature, salinity, dissolved oxygen, pH, and depth. The morphometric characteristic of P. margaritifera oyster in Arakan waters have shell length (PC) ranging from 44,25 – 169,77 mm with an average of 86,50 mm, Mantehage Bango waters  have PC ranged from 38,73 – 106,09 mm with an average of 80,31 mm, and Talengen waters have PC ranged from 25,89 mm to 105,34 mm with an average of 68,97 mm. Arakan waters are dominated by medium size P. margaritifera (53,78%), it is suspected that Arakan waters have pearl oyster stocks in nature for aquaculture activities. Evaluation of the suitability of waters for pearl oyster culture P. margaritifera in Arakan waters scored 77,7% categorized as suitable (S2), the location of Mantehage Bango waters scored 80% categorized as suitable (S2) and the location of Talengen waters 68,8% categorized as conditional (S3).
A POPULATION STUDY OF BLUE PILCHARDS (Sardinops neopilchardus) IN SOUTH AUSTRALIA WATER USING ALLOZYME ELECTROPHORESIS Winda Mercedes Mingkid
JOURNAL OF COASTAL DEVELOPMENT Vol 9, No 1 (2005): Volume 9, Number 1, Year 2005
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (185.488 KB)

Abstract

The biology and population analysis of Australian pilchards (Sardinops eopilchardus) was reviewedand studied. The Australian pilchards stock was depleted in South Australia whereas in other statesare under-exploited. The population study was done using protein analysis. Six enzymes (PEPB,AH, PGM, EST, MPI and AAT), which showed polymorphism, were scored and used in thepopulation study. For future studies, frozen procedure techniques need to be improved and numberof samples collected need to be extended. 
Co-Authors Aditya Sasamu Adnan Sjaltout Wantasen Adnan Wantasen Ali Djamhuri Antonius P Rumengan Bawekes, Frisley Rici Billy Th Wagey Calvyn F. A. Sondak, Calvyn F. A. Carolus P Paruntu Christo V S Aer, Christo V S Christo V.S Aer, Christo V.S Cyska Lumenta Cyska Lumenta Cyska Lumenta Dahlia Malau Diane J. Kusen Edwin D Ngangi Erly Y. Kaligis Erwin Wiradita Mamonto Farnis Boneka Fransine B. Manginsela Gompi, Wiranto Hariyani Sambali Haryani Sambali Hengky J. Sinjal Hengky J. Sinjal Hengky Sinjal Henky Manoppo Henneke Pangkey Henneke Pangkey Henneke Pangkey Janny Kusen Jeffrie F. Mokolensang Jeti K. Rangan Jhonly Solang Jimmy Mamuaya John L. Tombokan Joppy Mudeng Joshian Nicolas William Schaduw Juliaan Ch. Watung Julius Sampekalo Junaidi Asnawi Kreckhoff, Reni L. Kusen, Diane J. Lady D. Khartiono, Lady D. Mamuko, Nelsi Mantiri, Desy M. H Mardiana M Massora Dudung Medy Ompi Metlin Boroallo Mikraim J. Kaseger Mokoginta, Leyda H. Muhammad Aris Nego E. Bataragoa Nego Elvis Bataragoa Nickson J. Kawung Noni Saliwati Sarifudin Ockstan J Kalesaran Ockstan J. Kalesaran Ockstan J. Kalesaran Ockstan Jurike Kalesaran Ockstan Kalesaran Ockstan Kalesaran Paputungan, Fikri Ramses Nainggolan Reinol Jacobs Reiny A. Tumbol Reiny A. Tumbol Rene Charles Kepel, Rene Charles Revol D Monijung Revol D. Monijung Reygina B.S Roring Robert A. Bara Sambali, Hariyani Sammy N. J. Longdong Sammy N. J. Longdong Sammy N.J. Longdong Sammy N.J. Longdong Sarif Hidayat Silalahi, Torang J. P. Suria Darwisito Suzanne L. Undap Tamrin Tamrin Tamrin Taufiq Abdullah Tununu, Riky R. Veibe Warouw Wahidin, Nurhalis