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All Journal Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Biology, Medicine, & Natural Product Chemistry
Rosita Dewi
University of Jember
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Neuroscience Public Health

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EKSTRAK ETHANOL KULIT BAWANG MERAH (ALLIUM CEPA L.) MEMPERBAIKI KERUSAKAN HATI TIKUS WISTAR YANG DIINDUKSI DIAZINON Nilam Cahya; Rosita Dewi; Muhammad Hasan; Dina Helianti
JURNAL KEDOKTERAN DAN KESEHATAN Vol 9, No 3 (2022)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/JKK.V9I3.18862

Abstract

Diazinon merupakan pestisida organofosfat yang paling umum digunakan untuk mengendalikan hama. Konsumsi produk pertanian yang mengandung residu diazinon mengakibatkan akumulasi diazinon dan metabolitnya pada hati dan menimbulkan stress oksidatif. Kulit bawang merah (Allium cepa L.) mengandung flavonoid kuersetin yang dapat mengikat radikal bebas, menginduksi aktivitas enzim yang berperan penting dalam perbaikan sel, dan meningkatan jumlah dan kapasitas antioksidan endogen. Penelitian ini bertujuan mengetahui efek ekstrak ethanol kulit bawang merah (EKBM) dalam memperbaiki kerusakan hati tikus Wistar yang diinduksi diazinon. Tikus dibagi menjadi kelompok normal, diazinon, dan EKBM. Diazinon diberikan dengan dosis 40 mg/kgBB selama 7 hari dilanjutkan dengan EKBM dengan dosis 600, 900, dan 1200 mg/kgBB selama 7 hari. Pengamatan mikroskopik dilakukan terhadap 100 sel hati secara acak pada setiap preparat. Skor kerusakan sel hati ditentukan berdasarkan kriteria skor Manja Roenigk. Hasil penelitian menunjukkan bahwa diazinon meningkatkan skor kerusakan sel hati (p<0,05) dan EKBM dengan dosis 1200 mg/kgBB dapat menurunkan skor kerusakan sel hati sama dengan kelompok normal. Sebagai kesimpulan, EKBM dengan dosis 1200 mg/kg BB memperbaiki kerusakan hati tikus yang diinduksi diazinon yang ditunjukkan dengan penurunan skor kerusakan sel hati hingga tingkat normal.
Molecular Method Optimization to Identify Plasmodium falciparum Multidrug Resistance 1 (pfmdr1) gene as a Predictor of Antimalarial Resistance Erma Sulistyaningsih; Rosita Dewi; Sheilla Rachmania; Irawan Fajar Kusuma; Muhammad Rizqi Kholifaturrohmy; Yunita Armiyanti; Sakinatus Sariroh Kholifaturrohmah; Made Prasanti Andriani
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.673-678

Abstract

Several approaches have been designed to control malaria, a disease with high morbidity and mortality, but they face some hurdles. Antimalarial resistance is one of the major challenges for malaria elimination, so the detection of antimalarial resistance is essential. Several molecular markers for antimalarial resistance have been identified, including Plasmodium falciparum multidrug resistance 1 (pfmdr1) gene. This study determined the optimization of molecular techniques to identify the pfmdr1 gene as an antimalarial resistance predictor in Indonesia. The study included patients diagnosed with uncomplicated or severe malaria originating from the health district of Kerom Regency, Papua Province, and Dr. Soebandi Hospital, Jember, East Java Province. Blood samples were collected in the Whatmann filer paper after informed consent. DNA was isolated from dried blood filter paper, and nested PCR was performed using a specific primer, the pfmdr1-A and pfmdr1-B genes. The PCR cycle was optimized based on previous studies. The pfmdr1-A has a similar setting to the earlier study, but the pfmdr1-B had a different optimum setting from the previous study with the annealing temperature of 57oC for nested-1 and 62oC for nested-2. This PCR setting could be used for further examination. The positive results of the amplification indicated the potential for antimalarial resistance in the parasite population. A study on the gene copy number and polymorphism is essential to determine the definitive conclusion on antimalarial resistance.
The Increase of White Pulp Diameter of Rat Spleen After CIDR1alpha-PfEMP1 Recombinant Protein Injection Risma Aulia Magdalena; Rosita Dewi; Irawan Fajar Kusuma; Sheilla Rachmania; Erma Sulistyaningsih
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.663-667

Abstract

Exploring new protein targets for malaria vaccine development is essential in supporting malaria control strategies. One such candidate is the cysteine-rich interdomain region of Plasmodium falciparum Erythrocyte Membrane Protein-1 (CIDR1alpha-PfEMP1). The spleen, as a lymphoid organ, plays a critical role in immune responses, where activation is indicated by increased white pulp diameter. This study aimed to evaluate the immune response in rats following CIDR1alpha-PfEMP1 recombinant protein injection by measuring the spleen’s white pulp diameter. Twelve male rats were divided into two groups: a control group receiving 0.9% NaCl and a treatment group receiving 150 microgramof CIDR1alpha-PfEMP1 protein. The recombinant protein was administered subcutaneously on days 0, 21, and 42. On day 56, the rats were euthanized, and their spleens were collected, processed, and stained with hematoxylin-eosin (HE). White pulp diameters were measured microscopically (100×) using ImageJ software. The mean white pulp diameter was 22.590±3.986 micrometer in the control group and 36.607±6.739 ?m in the treatment group. Statistical analysis using the independent t-test showed a significant difference (p = 0.003). These results suggest that CIDR1alpha-PfEMP1 recombinant protein stimulates immune activity, as indicated by increased white pulp diameter in rat spleens.
Co-Authors Dina Helianti Erma Sulistyaningsih Irawan Fajar Kusuma Made Prasanti Andriani Muhammad Hasan Muhammad Rizqi Kholifaturrohmy Nilam Cahya Risma Aulia Magdalena Sakinatus Sariroh Kholifaturrohmah Sheilla Rachmania Yunita Armiyanti