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Dina Helianti
Department Of Histology, Faculty Of Medicine, University Of Jember, Jember, Indonesia
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Aktifitas Fisik Berat Menyebabkan Degenerasi Sel Hepatosit melalui Mekanisme Stres Oksidatif hairrudin, Hairrudin; Helianti, Dina; Widiastuti, Yulestrina
Jurnal Kedokteran Brawijaya Vol 27, No 2 (2012)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2012.027.02.2

Abstract

Hepar mempunyai peranan yang penting dalam mempertahankan homeostasis metabolisme tubuh. Stres oksidatif akibat aktifitas fisik berat dapat meningkatkan produksi Reactive Oxygen Species (ROS) yang kemudian menyebabkan kondisi patologis pada sel hepatosit, ditandai dengan perubahan struktur dan fungsinya. Penelitian ini bertujuan untuk mengetahui dampak stres oksidatif akibat aktifitas fisik berat terhadap kerusakan sel hepatosit. Penelitian ini menggunakan 16 ekor tikus putih Wistar jantan yang berumur 3 bulan, dengan berat badan antara 150 s.d. 200 gram yang dibagi ke dalam kelompok kontrol dan perlakuan. Aktifitas fisik berat yang berupa renang dengan beban dan intensitas maksimal diberikan pada kelompok kontrol, dilakukan setiap pagi selama 30 hari. Penilaian stres oksidatif ditentukan melalui pengukuran kadar MDA serum menggunakan teknik Thiobarbituric Acid Reactive Substance (TBARS). Perubahan histopatologi sel hepatosit berupa degenerasi sel dinilai berdasarkan klasifikasi sistem skoring NAFLD dari the Pathology Committee of the NASH Clinical Research Network. Rerata kadar MDA pada kelompok kontrol sebesar 42,38 nmol/ml, sedangkan pada kelompok perlakuan sebesar 89,63 nmol/ml. Pada pengamatan histopatologi hepar menunjukkan adanya proses degenerasi sel. Dapat disimpulkan bahwa aktifitas fisik berat dapat menyebabkan degenerasi sel hepatosit melalui mekanisme stres oksidatif.Kata Kunci: Aktifitas fisik berat, degenerasi, sel hepatosit, stres oksidatif.
Effect of Cacao on Plasma F2-Isoprostane Level, CD34 and ICAM-1 Expression of Coronary Arteries in Cigarette Smoking Exposed Rats Dina Helianti; Soetjipto; Widjiati; I Ketut Sudiana
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15729

Abstract

This research aimed to evaluate cardioprotective effects of cacao in smoking exposure condition that the effects were mediated through the anti-oxidant pathway by measure plasma F2-isoprostane level, Endothelial Progenitor Cell (EPC) enhancement by expression of CD34, while dysfunction endothelial condition was measured by expression of ICAM-1 in coronary arteries. This study subjected rats, divided into four groups: the normal control group (2 ml of aqua bidest, air exposure); the cigarette control group (2 ml of aqua bidest, cigarette smoke); cacao group 1 (1205 mg/kg BW/day, cigarette smoke); cocoa group 2 (2410 mg/kg BW/day, cigarette smoke). The oxidant biomarker, F2-isoprostane level was assessed using ELISA; CD34, and ICAM-1 expression in coronary arteries by immunohistochemistry. Cacao 1205 mg/kg BW/day significantly decreases plasma F2-isoprostane level, and ICAM-1 expression of coronary arteries in cigarette smoking exposed rat (p < 0.05) but there was not a significant increases CD34 (p < 0.05). Cocoa in cigarette smoke-exposed rats can prevent endothelial dysfunction through decrease F2-isoprostane but not increase CD34. The results of this study can be used as a basis for preventing endothelial dysfunction due to cigarette smoke by using cacao.
Shallot (Allium cepa L.) Skin Ethanol Extract Neutralizes Liver Oxidative Stress in Diazinon-Induced Wistar Rats Shahifa Audy Rahima; Rosita Dewi; Nindya Shinta Rumastika; Dina Helianti
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 6, No 1 (2022): Journal Qanun Medika Vol 6 No 01
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v6i1.8038

Abstract

ABSTRACTDiazinon has a hepatotoxic effect since it is metabolized in the liver involving Kupffer cells (KCs) activation, increasing reactive oxygen species (ROS). The flavonoids contained in shallot (Allium cepa L.) skin act as antioxidants neutralizing oxidative stress. This study aims to determine the effect of shallot skin ethanol extract (SSEE) on liver MDA level and activated KCs histopathology. The total flavonoids level of SSEE was measured using the aluminum chloride colorimetric method and resulted in 228.1 mg QE/g. Rats were divided into normal, diazinon, and SSEE groups. Diazinon was administered at a dose of 40 mg/kg b.w. for 7 days, followed by SSEE at the dose of 600, 900, and 1,200 mg/kg b.w. for 7 days. Liver malondialdehyde (MDA) level was measured using the MDA-TBA method. The results revealed that diazinon increased liver MDA level (p <0.05), while SSEE at doses of 900 and 1,200 mg/kg b.w. decreased liver MDA level equal to normal (p>0.05). Activated KCs in the SSEE group at a dose of 1,200 mg/kg b.w. was impressively equal to the normal group. In conclusion, SSEE at dose of 1,200 mg/kg b.w. neutralize liver oxidative stress due to diazinon indicated by the decrease of liver MDA level and activated KCs equal to normal.Keywords: antioxidant, diazinon, Kupffer cell, liver, MDA, shallotCorrespondence: rosita.fk@unej.ac.id
The Effect of Shallot Peel Ethanol Extract in Reducing Kidney MDA Levels in Diazinon-induced Wistar Rats Mukhammad Arif Hadi Khoiruddin; Dina Helianti; Muhammad Hasan; Rosita Dewi; Sheilla Rachmania
Journal of Agromedicine and Medical Sciences Vol 8 No 2 (2022)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v8i2.27424

Abstract

Diazinon is an organophosphate pesticide often used by farmer. The residue of diazinon may contaminate agricultural products and if accidentally consumed in a long term, may potentially led to health problems such as kidney disorders. Shallot (Allium cepa) peel was recently proposed to help recover the kidney damage due to its high flavonoid antioxidant content. This study aims to determine the antioxidant effect of shallot peel extract in reducing oxidative stress caused by diazinon with malondialdehyde (MDA) level indicator on the kidney of Wistar rats (Rattus norvegicus). This research was a true experimental with an in-vivo posttest-only control group design. The 28 rats were divided using a simple random sampling technique. The groups were the control group (K0), the diazinon group (K1), SPEE (Shallot Peel Ethanol Extract) groups which are P1 (300 mg/kg BW), P2 (600 mg/kg BW), P3 (900 mg/kg BW), P4 (1200 mg/kg BW) and P5 (2400 mg/kg BW). At the end of the study, the MDA kidney levels was analysed using the TBARS method. Effective doses were found in 600 mg/kg BW/day, 900 mg/kg BW/day, 1200 mg/kg BW/day, and 2400 mg/kg BW/day. The optimal dose of shallot peel extract in this study was 600 mg/kg BW/day. This study showed the effectiveness of shallot peel extract in reducing kidney MDA levels in diazinon-induced Wistar rats. Keywords: shallot peel, diazinon, kidney MDA level
EKSTRAK ETHANOL KULIT BAWANG MERAH (ALLIUM CEPA L.) MEMPERBAIKI KERUSAKAN HATI TIKUS WISTAR YANG DIINDUKSI DIAZINON Nilam Cahya; Rosita Dewi; Muhammad Hasan; Dina Helianti
JURNAL KEDOKTERAN DAN KESEHATAN Vol 9, No 3 (2022)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/JKK.V9I3.18862

Abstract

Diazinon merupakan pestisida organofosfat yang paling umum digunakan untuk mengendalikan hama. Konsumsi produk pertanian yang mengandung residu diazinon mengakibatkan akumulasi diazinon dan metabolitnya pada hati dan menimbulkan stress oksidatif. Kulit bawang merah (Allium cepa L.) mengandung flavonoid kuersetin yang dapat mengikat radikal bebas, menginduksi aktivitas enzim yang berperan penting dalam perbaikan sel, dan meningkatan jumlah dan kapasitas antioksidan endogen. Penelitian ini bertujuan mengetahui efek ekstrak ethanol kulit bawang merah (EKBM) dalam memperbaiki kerusakan hati tikus Wistar yang diinduksi diazinon. Tikus dibagi menjadi kelompok normal, diazinon, dan EKBM. Diazinon diberikan dengan dosis 40 mg/kgBB selama 7 hari dilanjutkan dengan EKBM dengan dosis 600, 900, dan 1200 mg/kgBB selama 7 hari. Pengamatan mikroskopik dilakukan terhadap 100 sel hati secara acak pada setiap preparat. Skor kerusakan sel hati ditentukan berdasarkan kriteria skor Manja Roenigk. Hasil penelitian menunjukkan bahwa diazinon meningkatkan skor kerusakan sel hati (p<0,05) dan EKBM dengan dosis 1200 mg/kgBB dapat menurunkan skor kerusakan sel hati sama dengan kelompok normal. Sebagai kesimpulan, EKBM dengan dosis 1200 mg/kg BB memperbaiki kerusakan hati tikus yang diinduksi diazinon yang ditunjukkan dengan penurunan skor kerusakan sel hati hingga tingkat normal.
Ekstrak Etanol Kulit Bawang Merah (Allium Cepa L.) Menurunkan Kadar Advanced Oxidation Protein Product (AOPP) Plasma Pada Tikus Wistar Terpapar Diazinon Sheilla Rachmania; Rosita Dewi; Dina Helianti; Ayu Munawaroh
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (434.966 KB) | DOI: 10.36418/syntax-literate.v7i10.9837

Abstract

Penggunaan diazinon secara ekstensif menimbulkan dampak buruk terutama bagi pekerja di bidang agroindustri. Efek karsinogenik dan toksik diazinon pada organ terjadi melalui jalur stres oksidatif. Flavonoid kuersetin yang terkandung dalam kulit bawang merah (Allium cepa L.) dapat menetralisir kondisi stres oksidatif, terlebih turunan kuersetin, 2-(3,4-dihydroxybenzoyl)-2,4,6-trihydroxy-3(2H)-benzofuranone (BZF), mempunyai efek antioksidan jauh lebih tinggi dibandingkan kuersetin. Studi ini bertujuan mengetahui efek antioksidan ekstrak etanol kulit bawang merah (EKBM) terhadap stres oksidatif pada tikus Wistar terpapar diazinon melalui pengukuran kadar AOPP plasma dan memprediksi target protein BZF. Sebanyak 25 ekor tikus Wistar jantan dibagi dalam kelompok normal, kontrol, dan tiga kelompok perlakuan. Diazinon per oral diberikan dengan dosis 40 mg/kgBB pada 7 hari pertama, dilanjutkan EKBM dengan dosis 600, 900, dan 1200 mg/kgBB pada 7 hari berikutnya. Kadar AOPP plasma diukur dengan metode kolorimetri Witko-Sarsat. Analisis karakterisasi BZF dilakukan dengan pendekatan bioinformatika Swiss Target Prediction. Kadar AOPP pada ketiga kelompok perlakuan secara signifikan lebih rendah dibandingkan kelompok normal (p<0,05) dan antara dosis 600 mg/kg BB dan 900 mg/kgBB menunjukkan perbedaan tidak bermakna (p>0,05). Karakterisasi BZF menghasilkan prediksi target protein, yaitu pada Kanal Potasium Kv1.3 (skor probabilitas >0,65) yang berperan dalam proses inhibisi reaksi oksidasi protein. Sebagai kesimpulan, pada penelitian ini EKBM dengan dosis 600 mg/kgBB paling efektif untuk menetralisir stres oksidatif akibat paparan diazinon yang ditunjukkan dengan penurunan kadar AOPP plasma. BZF diprediksi memiliki target protein dengan probabilitas tertinggi, yaitu pada Kanal Potasium Kv1.3.
Ekstrak Ethanol Kulit Bawang Merah (Allium cepa L.) Memperbaiki Kerusakan Hati Tikus Wistar yang Diinduksi Diazinon Nilam Cahya; Rosita Dewi; Muhammad Hasan; Dina Helianti
Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Vol. 9 No. 3 (2022): Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universi
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/jkk.v9i3.293

Abstract

Diazinon merupakan pestisida organofosfat yang paling umum digunakan untuk mengendalikan hama. Konsumsi produk pertanian yang mengandung residu diazinon mengakibatkan akumulasi diazinon dan metabolitnya pada hati dan menimbulkan stres oksidatif. Kulit bawang merah (Allium cepa L.) mengandung flavonoid kuersetin yang dapat mengikat radikal bebas, menginduksi aktivitas enzim yang berperan penting dalam perbaikan sel, dan meningkatan jumlah dan kapasitas antioksidan endogen. Penelitian ini bertujuan mengetahui efek ekstrak ethanol kulit bawang merah (EKBM) dalam memperbaiki kerusakan hati tikus Wistar yang diinduksi diazinon. Tikus dibagi menjadi kelompok normal, diazinon, dan EKBM. Diazinon diberikan dengan dosis 40 mg/kgBB selama 7 hari dilanjutkan dengan EKBM dengan dosis 600, 900, dan 1200 mg/kgBB selama 7 hari. Pengamatan mikroskopik dilakukan terhadap 100 sel hati secara acak pada setiap preparat. Skor kerusakan sel hati ditentukan berdasarkan kriteria skor Manja Roenigk. Hasil penelitian menunjukkan bahwa diazinon meningkatkan skor kerusakan sel hati (p<0,05) dan EKBM dengan dosis 1200 mg/kgBB dapat menurunkan skor kerusakan sel hati sama dengan kelompok normal. Sebagai kesimpulan, EKBM dengan dosis 1200 mg/kg BB memperbaiki kerusakan hati tikus yang diinduksi diazinon yang ditunjukkan dengan penurunan skor kerusakan sel hati hingga tingkat normal.
Analysis of Socio-Demographic Factors Influencing Family Latrine Utilization Behaviour Erna Hartatik; Rondhianto Rondhianto; Dina Helianti
NurseLine Journal Vol 8 No 2 (2023): November 2023
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/nlj.v8i2.43503

Abstract

Background: Providing healthy latrines for each family is an effort to reduce open defecation. However, there are still many open defecation incidents, which can increase the risk of fecal-oral transmission of disease. Many factors influence open defecation behavior, one of which is socio-demographic factors. Purpose: This study aims to analyze socio-demographic factors' influence on family latrine utilization behavior. Method: This type of research is observational analytic with a cross-sectional design. The sample size was 138 respondents using the cluster random sampling technique. The independent variables are age, gender, education, income, and number of family members. Meanwhile, the dependent variable is the behavior of using the family toilet. The instrument used was a questionnaire that was analyzed using multiple logistic regression and mean difference tests. Results: Most respondents were aged 41 – 60 years (73.2%), women (57.3%), secondary education (55.8%), with family members of less than four people (81.88%), and all respondents earned less than the minimum wage (100%). Most respondents had family latrine utilization behavior in the high category (66.7%). The modeling test showed the model fit (p = 0.034; R2 = 0.162). It showed that age, gender, education, income and number of family members simultaneously significantly affect family latrine utilization behavior by 16.2%. However, partially, only the educational factor had a significant effect (p=0.001). In addition, the mean difference test shows significant differences in family latrine utilization behavior based on age and education level (p=0.002; p=0.001). Conclusions: The socio-demographic factor that significantly influences latrine use behavior is the level of education. In carrying out health promotion efforts to increase family latrine utilization behavior, health workers should consider educational level factors to achieve the goal and reduce open defecation behavior.
Household Latrine Utilization Behavioral Model as an Effort to Improve Open Defecation-Free Program Hartatik, Erna; Rondhianto, Rondhianto; Helianti, Dina
Jurnal Promkes: The Indonesian Journal of Health Promotion and Health Education Vol. 12 No. 2 (2024): Jurnal Promkes: The Indonesian Journal of Health Promotion and Health Educatio
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jpk.V12.I2.2024.188-200

Abstract

Background: Healthy latrine-using behavior indicates healthy behavior. However, many people still practice open defecation, which can cause illness and increase death risk. Self-efficacy is a dominant predictor of health behavior change. Aims: The study aims to develop a household latrine utilization behavior model based on self-efficacy to improve an open defecation-free (ODF) program. Method: The study was a descriptive-analytic with a cross-sectional design. The sample size was 138 respondents with cluster random sampling. The study variables were endogenous (household latrine utilization), exogenous (predisposing, enabling, and reinforcing factors), and intervening variables (self-efficacy). Data were collected by questionnaire and analyzed using SEM-PLS. Result: The household latrine utilization behavior-based self-efficacy model is fit (SRMR=0.098; NFI= 0.910; Q2=0.334). Valid and reliable indicators were education (predisposing), clean water availability and latrine maintenance (enabling), and health worker and family support (reinforcing). Path analysis showed exogenous factors, directly and indirectly, influence latrine utilization behavior through self-efficacy. Predisposing and reinforcing factors had a direct and significant effect (p = 0.025; p = 0.001) while enabling factors were insignificant (p = 0.438). Enabling and reinforcing factors indirectly and significantly affected latrine utilization behavior through self-efficacy (p = 0.033; p = 0.004), while predisposing factors were insignificant (p = 0.141). Self-efficacy significantly influenced latrine-using behavior (p = 0.023). Conclusion: The household latrine utilization behavior-based self-efficacy model is a fit model with good predictive relevance in predicting household latrine use behavior. Health workers can use the model to enhance household latrine utilization behavior and improve the ODF program and public health status.
Antioxidant Effect of Shallot Peel Infusion on Kidney Oxidative Stress in Diazinon-Induced Wistar Rats Helianti, Dina; Rosita Dewi; Ayu Munawaroh Aziz; Sheila Rachmania
Jurnal Kesehatan dr. Soebandi Vol 11 No 2 (2023): Jurnal Kesehatan dr. Soebandi
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jkds.v11i2.394

Abstract

Diazinon is a pesticide widely used by farmers to control pests. Exposure to the low doses of diazinon can occur continuously through a polluted environment and diazinon residues in agricultural products. It has a nephrotoxic effect through oxidative stress mechanism. Flavonoid as antioxidant can significantly neutralize oxidative stress. Shallot peel is a source of flavonoids. This study was designed to determine the antioxidant effect of shallot skin infusion (SPI) on kidney oxidative stress in diazinon-induced Wistar rats by measuring the flavonoid level of SPI and kidney malondialdehyde (MDA) level. Rats were divided into normal, diazinon, and SPI groups. Diazinon was administered at dose of 40 mg/kgBW for 7 days followed by SPI at doses of 500, 1,000, and 2,000 mg/kgBW for 7 days. The flavonoid level of SPI was measured using spectrophotometry method and the kidney MDA level was measured using ELISA method. The flavonoid level of SPI was 96.8 mg QE/L. The results showed that the normal group had the lowest kidney MDA level of 2.585 mM/mL, the diazinon group had the highest level of 2.708 M/mL, while the SPI group at dose of of 500, 1,000, and 2,000 mg/kgBW had renal MDA levels of 2.642 mM/mL, 2.644 mM/mL, and 2.593 mM/mL. Nevertheless, the result of statistical analysis showed that the kidney MDA levels seven days after diazinon administration was not significantly different from the normal group so that SPI administration did not affect the kidney MDA level in diazinon-induced Wistar rats. Keyword: Shallot Peel, Diazinon, Oxidative Stress, Kidney Malondialdehyde
Co-Authors Aditya Kurniawan Aini, Nada Qurrota Aprilya Pratiwi, Nadilla Arifah, Naila Zanubah Ayu Munawaroh Aziz, Ayu Munawaroh Bagus Hermansyah Bintang, Muhammad Farrel Ravidinata Masoga Bi’izzyk, Avie Baldana Cholis Abrori, Cholis Dermawan, Rio Dini Agustina Erfan Efendi Erna Hartatik Hadi Paramu Hairrudin Hairrudin Hartatik, Erna Khairi, Aji Rayyan Khairi Maulana, Aditha Satria Muhammad Hasan Muhammad Hasan Muhammad Hasan Mukhammad Arif Hadi Khoiruddin Munawaroh, Ayu Nilam Cahya Nilam Cahya Nindya Shinta Rumastika Pralampita, Pulong Wijang Rahmadhan, Adiz Dwiputra Rista Setiani Rondhianto Rondhianto Rosita Dewi Rosita Dewi Rosita Dewi Rosita Dewi Rosita Dewi S.Pd. M Kes I Ketut Sudiana . septa surya wahyudi, septa surya Shahifa Audy Rahima Sheila Rachmania Sheilla Rachmania, Sheilla Singgih Bektiarso SOETJIPTO Sudarti Sudarti Sumadi Sumardi . Tania Ardiani Ulfa Elfiah Wahyu Muldayani Widjiati Yudha Nurdian Yulestrina Widiastuti Yunita Armiyanti