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All Journal Jurnal Riset Kesehatan Poltekkes Depkes Bandung Jurnal Pengabdian Masyarakat Kesehatan Indonesia
Betty Nurhayati
Poltekkes Kemenkes Bandung
Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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PENGARUH LAMA SIMPAN DAN KONSENTRASI MANITOL PADA PACKED RED CELL SAGM TERHADAP KADAR HEMOGLOBIN DAN HEMATOKRIT Indah Indah; Betty Nurhayati; Nina Marliana; Eem Hayati
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 14 No 2 (2022): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v14i2.2008

Abstract

Penyimpanan Packed Red Cell dapat menyebabkan perubahan biofisik dan biokimia, perubahan tersebut antara lain penurunan stabilitas eritrosit yang diukur dengan pelepasan hemoglobin dan dapat mempengaruhi nilai hematokrit. Penambahan zat preservatif ditujukan untuk mencegah terjadinya pembekuan darah dan menjaga fungsi sel. Dengan penambahan larutan aditif, dapat memberikan volume dan nutrisi tambahan untuk penyimpanan yang lebih lama dan aliran yang lebih baik. Larutan aditif yang banyak digunakan adalah SAGM yang terdiri dari saline, adenine, glucose dan mannitol. Lisisnya eritrosit dapat diminimalkan dengan cara menambahkan 1-2% manitol yang dapat mengurangi lisis sampai 50%. Larutan aditif akan mendukung kelangsungan hidup eritrosit sehingga dapat disimpan hingga 42 hari. Tujuan dari penelitian adalah untuk mengetahui pengaruh penyimpanan pada Packed Red Cell SAGM terhadap kadar hemoglobin dan hematokrit serta untuk mengetahui pengaruh konsentrasi manitol pada Packed Red Cell SAGM terhadap kadar hemoglobin dan hematokrit. Jenis penelitian yang dilakukan adalah eksperimen dengan penelitian quasy experimental. Desain penelitian adalah dengan rancangan acak lengkap dengan memberikan perlakuan lama penyimpanan Packed Red Cell (PRC) SAGM selama 0 hari, 14 hari dan 28 hari dan variasi konsentrasi manitol 0,525%, 1% dan 1,5% terhadap kadar hemoglobin dan nilai hematokrit. Data penelitian yang didapatkan dianalisis secara statistik menggunakan General Linear Model Repeated Measures Two Way, didapatkan hasil pemeriksaan kadar hemoglobin dan hematokrit nilai Asymp. Sig > 0,05. Dengan demikian dapat disimpulkan bahwa lama penyimpanan dan konsentrasi manitol Packed Red Cell SAGM tidak berpengaruh terhadap kadar hemoglobin dan hematokrit.
DESAIN PRIMER GEN PENGKODE RNA DEPENDENT RNA POLIMERASE (RdRp) UNTUK DETEKSI SARS COV2 DENGAN MENGGUNAKAN REAL TIME POLYMERASE CHAIN REACTION Fusvita Merdekawati; Betty Nurhayati
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 15 No 1 (2023): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v15i1.2179

Abstract

Corona virus or severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a virus that attacks the respiratory system. Disease caused by infection with this virus is called Corona Virus Disease 19 (COVID-19). Infection due to SARS-CoV-2 was declared by the World Health Organization (WHO) as a pandemic. Detection of infection due to SARS COV2 is carried out using Real Time Polymerase Chain Reaction by detecting the viral RNA sequence as the gold standard. One of the targeted viral genes is RNA-dependent RNA polymerase (RdRp). Detection using PCR consists of nucleic acid isolation, DNA amplification using qPCR and amplification curve reading. Testing for SARS-COV2 using molecular techniques uses several reagents that have been recommended by WHO. These reagents are special and close system where the master mix reagent component has been mixed with the primer so you cannot use reagents in general. Primer is an important component in a molecular detection system. Primer functions as a barrier for the target DNA fragment to be amplified. Each target fragment requires a pair of primers that match the target DNA. This primer needs to be designed to achieve a high success rate in detecting SARS-COV2. In silico primer design will make it easier to obtain good primers for the amplification of gene fragments. Based on the research results, the forward primer sequence was ACCGTAGCTGGTGTCTCTAT and the reverse primer sequence was GTGCCAACCACCATAGAATTTG. Furthermore, an in vitro detection process was carried out to test the success of the primer in forming the desired product. Based on the research results, the primer can stick to the DNA template as evidenced by the formation of an amplification curve with a CT value of 21,627 with optimal PCR conditions through the following stages: Reverse transcription at 37°C, 15 minutes, 1 cycle, Inactivation of Reverse transcriptase and Activation of DNA Polymerase at 95°C for 10 minutes, 1 cycle, Denaturation at 95°C for 10 seconds, 40 cycles, annealing at 56°C for 10 seconds 40 cycles. Followed by the extension stage at 72°C for 30 seconds for 1 cycle.
PEMBERDAYAAN MASYARAKAT MELALUI PEMANFAATAN TANAMAN KENIKIR DALAM UPAYA PENCEGAHAN KANKER DI RW16 DAN 17 KELURAHAN CIPAGERAN KECAMATAN CIMAHI UTARA Eem Hayati; Betty Nurhayati; Sonny Feisal Rinaldi
Jurnal Pengabdian Masyarakat Kesehatan Indonesia Vol. 1 No. 1 (2022): Jurnal Pengabdian Masyarakat Kesehatan Indonesia
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jpmki.v1i1.971

Abstract

Cancer is a disease that occurs due to the uncontrolled growth of abnormal cells, causing normal body tissues to be damaged. There are several ways that can be done to prevent cancer, including paying attention to and implementing a healthy diet and lifestyle. Some medicinal plants have the potential as anti-cancer that has a cytotoxic effect, one of which is kenikir. Kenikir leaves contain active compounds of alkaloids, polyphenols, saponins, tannins and flavonoid glycosides quercetin. The results of previous studies by several researchers showed that several methanolic extracts from kenikir leaves were known to have a cytotoxic effect on T47D cells and kenikir ethanol extract was proven as an antioxidant and anticancer of the uterus. The purpose of community service is to apply the potential of kenikir leaves as an anti-cancer so that it can be used by the community in RW 16 and 17 Cipageran Village, North Cimahi District. The methods used are counseling to cadres and planting kenikir plant seeds at partner locations. The results of community service obtained that the pre-test and post-test scores in RW 16 were 53 and 93, there was an increase of 76%, while the pre-test and post-test scores in RW 17 were 56 and 88, there was an increase of 57%. The conclusion of the PKM results is that there is an increase in the knowledge and understanding of the cadres regarding the use of kenikir plants in an effort to prevent cancer.  
Co-Authors Eem Hayati Fusvita Merdekawati Indah Indah Nina Marliana Sonny Feisal Rinaldi