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All Journal International Journal of Agriculture System
Gemilang Rahmadara
Research Center for Genetic Engineering, OR HL, BRIN, KST Soekarno, Jl. Raya Jakarta-Bogor Km 46, Cibinong 16911
Agriculture, Biological Sciences & Forestry

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Comparison of DNA Isolation Methods that Derived from Leaves of a Potential Anti-Cancer Rodent Tuber (Typhonium flagelliforme) Plant Gemilang Rahmadara; Nurul Fitri Hanifah; Rismayanti Rismayanti; Devit Purwoko; Andi Rochandi; Teuku Tajuddin
International Journal of Agriculture System VOLUME 10 ISSUE 2, DECEMBER 2022
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijas.v10i2.3966

Abstract

The content of polysaccharides, polyphenols, proteins, and RNA compounds is the main problem often found in Plants DNA isolation, which inhibit the process of DNA isolation. Comparing the methods of plant DNA isolation is necessary for obtaining the DNA with good quality, purity, high concentration and efficiency time and cost. This study aimed to determine the best DNA isolation method that derived from leaves of a potential anti-cancer Rodent Tuber (Typhonium flagelliforme) plant by comparing the conventional DNA isolation method (cetyl trimethyl ammonium bromide/CTAB) and 2 commercial kits (Promega Wizard™ Genomic DNA Purification Kits, and Geneaid Genomic Mini Kit). The results showed that the CTAB method yielded a higher amount of DNA (>100 ng/µL) at the cost of 0.49 USD per sample, in comparison with Promega method (69.19 to 157.68 ng/µL) at 3.28 USD per sample and Geneaid method (8.15 to 18.52 ng/µL) at 2.06 USD per sample. Based on the purity of isolated DNA (A260/280), CTAB method produced relatively similar DNA quality to Promega kit (1.8-2.0). On the other hand, Geneaid method resulted in a lower purity value at 1.15 to 1.60.
Co-Authors Andi Rochandi Devit Purwoko Nurul Fitri Hanifah Rismayanti Rismayanti Teuku Tajuddin