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UJI DAYA HAMBAT MADU HUTAN BADUY SEBAGAI SUBSTRAT PADA BUNGA TELANG (Clitoria ternatea L) MELALUI METODE BIOTEKNOLOGI FERMENTASI KOMBUCHA DALAM MENGHAMBAT PERTUMBUHAN BAKTERI PATOGEN Kusumiyati Kusumiyati; Diyan Yunanto Setyaji; M. Fariz Fadillah; Firman Rezaldi
MEDFARM: Jurnal Farmasi dan Kesehatan Vol 11 No 2 (2022): Medfarm: Jurnal Farmasi dan Kesehatan
Publisher : LPPM Akafarma Sunan Giri Ponorogo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.48191/medfarm.v11i2.109

Abstract

Telang flower (Clitoria ternatea L) fermented by kombucha is a probiotic drink produced by a consortium of bacteria and yeast. The raw material in this kombucha fermentation is in the form of telang flowers that have been cold from the previous boiling process. This study aims to determine the antibacterial activity of telang flower kombucha with different concentrations of Baduy forest honey. The concentration of Baduy forest honey consisted of 20%, 30%, and 40% (w/v) where each treatment was repeated 3 times. The positive control used in this study was kombucha made from green tea. Sterile aquadest is a treatment positioned as a negative control. The disc diffusion method is one of the methods used in antibacterial testing by measuring the diameter of the inhibition zone. Telang flower kombucha fermentation has activity as a broad-spectrum antibacterial source. This can be indicated by the potential to prevent the growth of Staphylococcus aureus, Staphylococcus epidermidis, Pseudomnonas aeruginosa, and Escherichia coli bacteria. Baduy forest honey concentration of 40% is the best concentration in fermented kombucha flower telang as an antibacterial and has the potential as the latest functional drink from fermented biotechnology products.
UJI DAYA HAMBAT PERTUMBUHAN BAKTERI GRAM POSITIF DAN NEGATIF DARI PRODUK BIOTEKNOLOGI FARMASI DALAM BENTUK FORMULASI DAN SEDIAAN SABUN CUCI PIRING GEL KOMBUCHA BUNGA TELANG (Clitoria ternatea L) Firman Rezaldi; Rustini Rustini; Ayu Safitri; Siska Dwi Anggraeni; Aris Ma'ruf; Eman Eman; Maya Puspitasari
AGRIBIOS Vol 21 No 1 (2023): JUNI
Publisher : Program Studi Agribisnis Fakultas Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/agribios.v21i1.2843

Abstract

Dishwashing gel soap made from telang flower kombucha fermented solution has the potential to inhibit the growth of gram-positive and negative bacteria. Gram-positive and negative bacteria that have been successfully inhibited by gel dish soap made from an active ingredient of telang flower kombucha fermented solution include Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Escherichia coli. This research is an experimental laboratory, namely by providing dishwashing soap that has been circulating in the market as a positive control. Making a gel hand washing soap base with an active ingredient of telang flower kombucha fermented solution at a sugar concentration of 20%, 30%, and 40% which was tested against the four test bacteria consisting of two gram-positive bacteria and 2 gram-negative bacteria using disc diffusion. The data analysis used was one-way ANOVA where the resulting P value was less than 0.05 and it can be concluded that the telang flower kombucha gel dish soap at a concentration of 40% based on further analysis in the form of a post hoc test, which is significantly different from the kombucha gel dish soap butterfly pea flower at concentrations of 20% and 30% inhibited the growth of the four test bacteria but was not significantly different from the dishwashing soap on the market as a positive control. The formulation and preparation of telang flower kombucha gel dish soap at a concentration of 40% was the best concentration in inhibiting the growth of the four test bacteria compared to concentrations of 20% and 30% and even the two comparison dish soaps.