Devanda Ayu Lidya Permata Putri
Department Of Soil Science, Faculty Of Agriculture, Universitas Pembangunan Nasional “Veteran” Yogyakarta, Yogyakarta, Indonesia

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Journal : Acta Solum

Keragaman Jamur Mikoriza Arbuskular pada Rizosfer Tiga Varietas Tanaman Tebu: Diversity of Arbuscular Mycorrhizal Fungi in the Rhizosphere of Three Sugarcane Varieties Zulfa Fatmawati; Jaka Widada; Donny Widianto; Anjar Cahyaningtyas; Devanda Ayu Lidya Permata Putri
Acta Solum Vol. 3 No. 2 (2025): Maret 2025
Publisher : Department of Soil, Faculty of Agriculture, Lambung Mangkurat University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/actasolum.v3i2.3293

Abstract

Arbuscular mycorrhizal fungi (AMF) form associations with more than 80% of the terrestrial plants. These associations enhance nutrient sequestration and plant resistance to environmental stress. The diversity of AMF is highly influenced by the host plants. The types of AMF in the Bululawang, Kidang Kencana, and Pasuruan Jengkol 922 sugarcane varieties grown in a similar location were identified through morphological and molecular methods. AMF spores were isolated by the wet sieving method. The morphological features of the AMF spore mounted in PVLG and Melzer’s reagent were observed under a microscope. The DNA of the fungi was extracted, and the ribosomal RNA genes were amplified by nested PCR with the NS1-NS4 primer pairs, followed by the AML1-AML2 primer pairs. A moderate diversity of AMF was observed, with a total of 269 spores found in the Kidang Kencana variety, 191 spores in the Bululawang variety, and 142 spores in the Pasuruan Jengkol 922 variety. Four AMF species have been identified morphologically and molecularly, namely Glomus flavisporum, Acaulospora koskei, Gigaspora Margarita, and Scutellospora savannicola. These findings indicate that the number and type of AMF associated with sugarcane roots are influenced by plant varieties, in which the Glomus sp. is the dominant species in the three sugarcane varieties studied.
Unveiling the BPF9 Isolate as a Potential of Phosphate-Solubilizing Bacteria Through In Vitro Characterization Devanda Ayu Lidya Permata Putri; Guruh Mayka Putra; Laudy Arrisa Arumsari Sahana; Intan Nirmalasari
Acta Solum Vol. 3 No. 3 (2025): Juli 2025
Publisher : Department of Soil, Faculty of Agriculture, Lambung Mangkurat University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/actasolum.v3i3.3329

Abstract

The increasing demand for food has driven agricultural intensification, particularly through the use of phosphate fertilizers. However, phosphorus uptake efficiency by plants remains low due to its predominance in insoluble forms in the soil. One promising alternative is the use of phosphate-solubilizing bacteria (PSB). This study aimed to evaluate the solubilization potential of the BPF9 isolate through qualitative and quantitative assays, as well as growth curve profiling. The research was conducted at the Soil and Environmental Biotechnology Laboratory, IPB University. Qualitative phosphate solubilization was assessed on Pikovskaya agar by measuring halo zone formation to calculate the solubilization index (SI). Quantitative analysis was carried out using spectrophotometry at 660 nm. The bacterial growth curve was analyzed using the haemocytometer method. Results showed that BPF9 had a solubilization index of 2.0 (moderate category) and was able to solubilize phosphate up to 128.5 ppm, which is considered highly efficient. The growth curve indicated that BPF9 entered the exponential phase at 26 hours and reached its maximum population at 42 hours. These findings indicate that BPF9 possesses strong phosphate-solubilizing capability and stable growth characteristics, making it a promising candidate for biofertilizer development.
Detection of Arbuscular Mycorrhizal Fungi in the Root of Allium fistulosum Grown in the Fumigated Field Anjar Cahyaningtyas; Devanda Ayu Lidya Permata Putri; Ummi Rosyidah; Zulfa Fatmawati
Acta Solum Vol. 3 No. 3 (2025): Juli 2025
Publisher : Department of Soil, Faculty of Agriculture, Lambung Mangkurat University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/actasolum.v3i3.3355

Abstract

Soil fumigation is important pre-plantation practices to maximize land productivity. Not only effectively eliminate soil pests and pathogens, but this practice also affecting the beneficial soil microbial community including arbuscular mycorrhizal (AM) fungi. The AM fungal community in the roots of Allium fistulosum were studied. The roots have been collected from fumigated and non-fumigated field. The DNA has been extracted, then the fungal LSU ribosomal RNA gene has been amplified and sequenced. Two and three AM fungal OTUs have been detected from fumigated and non-fumigated field, respectively. Decomposer and pathogenic fungi were detected coexist with the AM fungi, suggesting the resilience of these fungi upon fumigation treatment.