Article Per Year (5 Year)
p-Index From 2020 - 2025
0.23
P-Index
This Author published in this journals
All Journal BALABA (JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA)
Zulfa Zahra Salsabila
Institut Teknologi Kesehatan dan Sains (ITKES) Wiyata Husada Samarinda, Jalan Kadrie Oening Gang Monalisa Nomor 77, Air Hitam, Kota Samarinda, Kalimantan Timur, Indonesia
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Public Health Veterinary

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Deteksi Plasmodium knowlesi Menggunakan Nested Polymerase Chain Reaction (PCR) di Kecamatan Muara Komam Kalimantan Timur Zulfa Zahra Salsabila; Rintis Noviyanti; Farah Coutrier; Leily Trianty; Eman Sutrisna; Lantip Rujito
BALABA: JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA Volume 18 Nomor 2 Desember 2022
Publisher : Balai Penelitian dan Pengembangan Kesehatan Banjarnegara Badan Litbangkes Kemenkes RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/blb.v18i2.6359

Abstract

Plasmodium knowlesi is a parasite of the genus plasmodium that naturally infects long-tailed macaques (Macaca fascicularis), but currently reported has ability to infect humans. The identification/detection of P. knowlesi can be done using RDT, microscopic, or molecular examinations using nested PCR. Nested PCR is the most sensitive and specific method of examination to date. This study aimed to detect P. knowlesi in humans by RDT, microscopic, and nested PCR examinations. The study was descriptive with a cross-sectional approach, carried out from March to July 2019. The samples in this study were 123 patients who were suspected of being infected with malaria and who underwent laboratory tests at the Muara Komam Health Center. Microscopic examination and RDT examination were carried out at the Muara Komam Health Center, while nested PCR was carried out at the Eijkman Molecular Biology Laboratory Jakarta. The results of RDT and microscopic examinations showed as many as 16 of 123 (13%) malaria-positive samples of P. falciparum and P. vivax, and 10 of 123 (8.1%) malaria-positive samples of P. falciparum and P. vivax. Nested PCR tests targeting the rRNA SSU gene were able to identify P. knowlesi by 6 out of 123 (4.87%). In conclusion, the study showed that Plasmodium knowlesi was detected in humans in Muara Komam, East Kalimantan through nested PCR examination.
Co-Authors Eman Sutrisna Farah Coutrier Lantip Rujito Leily Trianty Rintis Noviyanti