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All Journal Jurnal Kedokteran Brawijaya MANDALA of Health Journal of Biomedicine and Translational Research Jurnal NERS MEDISAINS Majalah Kedokteran Universa Medicina Jurnal Pengabdian Pada Masyarakat Dinamisia: Jurnal Pengabdian Kepada Masyarakat Molekul: Jurnal Ilmiah Kimia Scripta Biologica Jurnal Informatika dan Rekayasa Elektronik Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia Journal Of Bionursing Jurnal Comprehensive Health Care BALABA (JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA) Indonesian Journal of Global Health research Insights in Public Health Journal JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Jurnal Riset Rumpun Ilmu Kedokteran (JURRIKE) Mandala of Health : A Scientific Journal Jurnal Keperawatan Soedirman (The Soedirman Journal of Nursing) Jurnal Ekonomi, Bisnis, dan Akuntansi Medical and Health Journal Indonesian Journal of Dentistry Karunia: Jurnal Hasil Pengabdian Masyarakat Indonesia Linggamas: Jurnal Pengabdian Masyarakat
Lantip Rujito
Departemen Genetika Dan Biologi Molekuler, Fakultas Kedokteran Universitas Jenderal Soedirman
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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HUBUNGAN ANTARA INDEKS MASSA TUBUH (IMT) DENGAN TES FUNGSI PARU Rujito, Lantip; Ristianingrum, Ika; Rahmawati, Indah
MANDALA of Health Vol 4, No 2 (2010): Mandala Of Health
Publisher : Jurusan Kedokteran FK Unsoed

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Abstract

Body mass index (BMI) is a tool of measuring the nutritional status of individu. One of the effects ofobesity is the mechanics disturbance led to abnormality on pulmonary function tests. This study was aimed todetermine the relationship between body mass index with pulmonary function tests (PFTs) on medical studentJenderal Soedirman University. Analityc observational study with cross sectional design was used in thisinvestigation. The sampling technique used proportional random sampling with 82 samples. Pulmonaryfunction tests was classified into vital capacity (VC), tidal volume (TV), inspiration reserve volume (IRV),expiratory reserve volume (ERV), inspiration capacity (IC), forced vital capacity (FVC) dan FEV1.Univariate analysis, which was using table of frequency to see chategorichal variables and central tendencymeasurement to see numerical variables, and bivariate analysis, which was using Pearson and Spearmancorrelation to see the relationship between BMI with PFTs; unpaired t tests and Mann Whitney to know thedifferences of PFTs results between men and women, were used in this research. From the results weconcluded that there are significant relationship between between BMI with VC (p = 0,015), IRV (p = 0,026),IC (p = 0,016), FVC (p = 0,004) and FEV1 (p = 0,021) with low power relationship and was no relationshipBMI with TV (p = 0,489) and ERV (p = 0,231). In the unpaired t test and Mann Whitney we found that thereare differences all PFTs in a group of men and women.
GFR and Blood Lead Levels in Gas Station Workers Based on δ-Alad Gene Polymorphisms Rujito, Lantip; Hanief, Muhammad Nur; Gozali, Paulus; Mulyanto, Joko
Jurnal NERS Vol 10, No 1 (2015): Vol. 10 Nomor 1 April 2015
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (332.002 KB) | DOI: 10.20473/%oj.Ners101%y74-79

Abstract

Introduction: Lead is a well-known toxic agent that makes an organ’s failure. Lead serum itself is infl uenced by δ-ALAD gene polymorphisms (Amino Levulinic Acid Dehydratase). δ-ALAD gene encodes an ALAD enzyme used for heme synthesis. The Characteristic of gene polymorphism may result in Glomerulo Filtration Rate (GFR) value as mark of renal failure. The goal of this study was to fi nd correlations between blood lead levels with GFR in terms of δ ALAD gene polymorphisms. Method: A cross-sectional design was used to perform this research. Thirty-eight gas stations workers in Banyumas were recruited in this study. δ-ALAD gene polymorphisms were characterized using PCR-RFLP method, while lead serum levels were quantifi ed by Atomic Absorption Spectrophotometer (AAS). In addition, Creatinin serum was done with a spectrophotometer and GFR value was formulated by means of the Schwartz method. Result: The studyshowed that the proportion of ALAD genotype for ALAD 1-1, 1-2 and 2-2 were 94.7%, 5.3%, and 0% respectively. The mean of serum levels in homozygous 1-1 was 15.94 ppb and heterozygote 1-2 was 1.15 ppb. GFR of participants ranged from 71.11 mL/min to 185.20 mL/min with a mean of 117.34mL/min. There was no correlation between serum Pb and GFR (p = 0.19). Study also could not determine the correlation between GFR and ALAD gene Polymorphism. Discussion: Study then concluded that there was no correlation between blood lead levels in the GFR on each δ-ALAD genotypes.Keywords: Lead intoxication, GFR, δ-ALAD, gas station workers
Hair root FMRP expression for screening of fragile X full mutation females Rujito, Lantip; Kustiani, Dwi; Severijnen, Lies Anne; Hanzon, Peter; Faradz, Sultana MH; Willemsen, Rob
Universa Medicina Vol 30, No 1 (2011)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2011.v30.11-21

Abstract

The fragile X syndrome is the most common form of inherited mental retardation in humans, caused by an expansion of the cytosine-guanine-guanine (CGG) repeat in the fragile X mental retardation 1 (FMR1) gene located on the X chromosome. Antibody tests have been developed to identify fragile X patients, based on the presence or absence of fragile mental retardation protein (FMRP) in both lymphocytes and hair roots. The objective of this study was to compare correlations of hair root and lymphocyte FMRP expression with cognitive functioning in female rural area probands carrying the full mutation. Thirty females (normal, premutation, or full mutation) were selected from Indonesian fragile X families and were tested for FMRP expression in lymphocytes and hair roots using the FMRP antibody test. Subject genotype was determined by Southern blot analysis, and IQ equivalent by Raven’s Standard Progressive Matrices. Statistical analysis was by Pearson correlation. FMRP expression in blood lymphocytes was relatively higher than that in hair roots, but hair root FMRP expression was strongly correlated with cognitive functioning in female full mutation carriers (r=0.64, p=0.015), whereas no significant correlation between lymphocyte FMRP and cognitive functioning was found (r=0.31, p= 0.281). Around 14% of subjects had a normal and 7% a borderline IQ level, while 79% had mild mental impairment. In conclusion, hair root FMRP expression may be a useful marker for identification of fragile X full mutation females.
GLUTATHIONE S TRANSFERASE AND CATALASE GENE POLYMORPHISMS DID NOT TEND TO INFLUENCE THE SEVERITY OF HEMOGLOBIN E/β-THALASSEMIA Rujito, Lantip; Widodo, Yundandhika Rizki; Sakina, Ghaida; Santosa, Qodri; Hapsari, Ariadne Tiara
Universa Medicina Vol 39, No 1 (2020)
Publisher : Faculty of Medicine, Trisakti University

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Abstract

BackgroundThalassemia, a monogenic genetic disease of red blood cells, is spread widely throughout the world. Glutathione S transferase (GST) enzymes have an antioxidant role in detoxification processes of toxic substances This study aimed to determine the role of the genetic modifier genes GSTT1 and GSTM1, and the catalase (CAT) gene in clinical degrees of hemoglobin (Hb)E/? thalassemia. MethodsSixty HbE/? Thalassemia patients were examined to determine their clinical pictures. Clinical score was based on age when thalassemia symptoms appeared, time of diagnosis, time of first blood transfusion, pre-transfusion hemoglobin concentration, frequency of transfusions, and enlargement of spleen. Ferritin concentration was also obtained from medical records. Gene polymorphisms of GSTT1, GSTM1, and CAT were measured using PCR and PCR-RFLP methods. Clinical scores were categorized into mild (0-3.5), moderate (4-7), and severe (7.5-10) degrees, while ferritin level was expressed in mg/dL. One way Anova was used to analyze the data. ResultsThe clinical appearance showed that severe, moderate, and mild degrees accounted for 42%, 45%, and 13%, respectively. The majority had a high ferritin level of more than 5000 mg/dL (67%). GSTT1 null, GSTM1 null, and CAT minor allele genotypes were 21.7%, 33.3%, and 12.1%, respectively. GSTT1, GSTM1, and CAT genotypes had no impact on the severity of thalassemia patients (p=0.091, p=0.082, and p=0.141, respectively).ConclusionGSTT1, GSTM1, CAT gene polymorphisms tend to be a minor aspect of severity of clinical outcome for HbE/â thalassemia patients and should be not considered a routine laboratory check.
Uji Diagnostik Indeks Darah dan Identifikasi Molekuler Karier Talasemia β pada Pendonor Darah di Banyumas Hapsari, Ariadne Tiara; Rujito, Lantip
Jurnal Kedokteran Brawijaya Vol 28, No 3 (2015)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (866.366 KB) | DOI: 10.21776/ub.jkb.2015.028.03.13

Abstract

Talasemia menempati kelainan genetik yang paling umum di seluruh dunia dengan prevalensi karier talasemia di Indonesia adalah sekitar 3-10%. Banyumas merupakan salah satu wilayah yang menyumbang angka prevalensi penderita talasemia yang cukup besar. Salah satu perangkat dalam program pencegahan terpadu adalah memastikan diagnosa molekuler pada tahap skrining sesuai dengan mutasi lokal. Tujuan dari penelitian adalah untuk mengetahui nilai uji diagnostik indeks darah dan karakterisitik mutasi talasemia β pada pendonor darah yang dicurigai karier talasemia. Subjek penelitian adalah 183 pendonor darah rutin pada PMI Banyumas. Skrining awal menggunakan indeks darah MCV, MCH, dan Hemoglobin elektroforesis. Karakteristik molekuler dilakukan dengan teknik PCR-RFLP dan teknik ARMS.  Hasil penelitian menunjukkan bahwa MCV memiliki nilai sensitivitas 81,3%, dan spesifisitas 95,8% sedangkan MCH menunjukkan sensitivitas sebesar 80% dengan spesifisitas 97,5%. Mutasi IVS-1 nt 5 (G>C) merupakan mutasi tersering disusul dengan HBE (codon 26) dan IVS1 nt 1(G>T).Kata Kunci: Identifikasi molekuler, indeks darah, karier talasemia
ANALISA VISUAL MENGGUNAKAN ETETOOLKIT FRAMEWORK TERHADAP PENYAKIT BETA-THALASSEMIA DI JAWA TENGAH BAGIAN SELATAN Lalu Mutawalli; Moh Reza Syaifur Rizal; Wayan Tunas Artama; Rohmatul Fajriyah; Izzati Muhimmah; Lantip Rujito
Jurnal Informatika dan Rekayasa Elektronik Vol. 2 No. 1 (2019): JIRE April 2019
Publisher : LPPM STMIK Lombok

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36595/jire.v2i1.75

Abstract

Deteksi peristiwa biomolekuler dalam visual yang akan dianalisis menggunakan komputasi untuk mendeteksi efektivitas dan akurasi penyakit. Sebagai hasil utama, banyak analisis visual, mulai dari pengelompokan gen hingga filogenetik, menghasilkan pohon hierarkis. Toolkit Lingkungan Eksplorasi Pohon (ETE) yang membantu manipulasi, analisis, dan visualisasi pohon hierarkis otomatis. Kemudian, dalam makalah ini, daftar mutasi β-thalassemia yang merupakan kelompok kelainan darah herediter yang ditandai oleh anomali dalam sintesis rantai beta hemoglobin yang menghasilkan berbagai fenotipe mulai dari anemia berat hingga individu tanpa gejala klinis. Hasil ini adalah ETEToolkit dapat menguraikan mutasi ini untuk ditampilkan melalui pohon dan penyelarasan dalam satu bingkai, kemudian kita dapat menyesuaikan dan merender ke dalam gambar PDF. Mutasi ini berlokasi di pusat Jawa, Indonesia.
Hubungan Indeks Massa Tubuh dengan Sindroma Prementruasi Rendi Retissu; Sjafril Sanusi; Amalia Muhaimin; Lantip Rujito
Majalah Kedokteran UKI Vol. 27 No. 1 (2010): JANUARI – MARET
Publisher : Fakultas Kedokteran Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/mkvol34iss2pp60

Abstract

Abstrak Sindrom premenstruasi adalah kumpulan gejala fisik dan psikologi yang terjadi sebelum masa menstruasi. Wanita lazim mengalami sindrom premenstruasi dengan prevalensi sebanyak 90%, dan 3-5% di antaranya mendapatkan gejala yang mengganggu kehidupan sehari-hari. Salah satu faktor risiko sindrom premenstruasi adalah indeks massa tubuh. Tujuan penelitian ini adalah untuk mengetahui hubungan antara indeks massa tubuh dan sindrom premenstruasi pada mahasiswi Fakultas Kedokteran Universitas Jenderal Soedirman. Penelitian ini adalah penelitian analitik observasional dengan desain cross sectional. Teknik sampling yang digunakan adalah proporsional random sampling, dengan jumlah subjek sebanyak 75 orang. Hasil analisis univariat menunjukkan, terdapat 53,3% wanita yang memiliki sindrom premenstruasi, sedangkan 46,7% lainnya tidak memiliki sindrom premenstruasi. Analisis bivariat menggunakan uji chi-kuadrat untuk menemukan hubungan antara indeks massa tubuh dengan sindrom premenstruasi. Hasil penelitian menunjukkan, bahwa terdapat hubungan antara indeks massa tubuh dengan sindrom premenstruasi (p = 0,026). Kata Kunci: sindrom pramenstruasi, indeks massa tubuh Abstract Premenstrual syndrome is a group of physical and psychological symptoms which occurs before the time of menstruation. In general, the female premenstrual syndrome prevalence is about 90%, whereas 3-5% of them experienced disturbance symptoms in the daily life. One of the premenstrual syndrome’s risk factors is the body mass index. The purpose of this study was to know the relationship between the body mass index and the premenstrual syndrome among female students of the Jenderal Soedirman University, School of Medicine. This study was an observational analytic research with cross sectional design. The sampling technique was proportional random sampling, enrolling 75 of subjects. The univariate analysis showed that there was 53,3% female who experienced the premenstrual syndrome, whereas 46,7% female didn’t experience it. The bivariate analysis chi-square test was used to find out the relationship between the body mass index and the premenstrual syndrome. The result showed that there was a relationship between the body mass index and the premenstrual syndrome (p=0,026). Key Words: premenstrual syndrome, body mass index
Genetic Background of β Thalassemia Modifier: Recent Update Lantip Rujito; Teguh Haryo Sasongko
Journal of Biomedicine and Translational Research Vol 4, No 1 (2018): July 2018
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (51.152 KB) | DOI: 10.14710/jbtr.v4i1.2541

Abstract

Thalassemia has become major health problem among developing countries. Genetic background which contain enormous mutations and variations have lead in clinical problem differences.The genetic basis of thalassemia, beta specifically, is mutations of the gene encoding the β chain of the hemoglobin (Beta-Globin, HBB). However, today it is known that abnormalities in this gene do not necessarily determine the clinical appearance of β thalassemia patients.A set of genes has been found that can modify the primary β thalassemia disorder. Secondary modifier contains genes that have been associated with elevated levels of HbF and improvement ratio of α / β globin chain. The genes involved are HBA, HBG, BCL11A, HBS1L-MYB and other cofactor genes regulating erythropoiesis. Tertiary genetic modifier comes from other genes related to the disease severity including iron metabolism, redox activity, and clinical complications. The review aims to provide the latest updates regarding the known β Thalassemia modifier genes and some other genes involved in the changes of the clinical manifestations.
Detection of icaAD Gene of Biofilm-Producing Staphylococcus aureus Carriage Isolates Obtained from Health Care Workers and Healthy Communities in Banyumas, Indonesia Gembong Satria Mahardhika; Metta Ayu Susanti; Lantip Rujito; Dwi Utami Anjarwati
Journal of Biomedicine and Translational Research Vol 6, No 1 (2020): April 2020
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v6i1.6135

Abstract

Background: Asymptomatic biofilm-producing Staphylococcus aureus carriage play a pivotal role as a reservoir pathogen and increase the transmission rate in hospital as well as in healthy community. Biofilm- producing S. aureus which is regulated by the the ica AD gene reduce the antimicrobial ability in eliminating the pathogen. Objective: The aim of this study was to detect the icaAD gene of biofilm-producing Staphylococcus aureus carriage isolates obtained from healthcare workers and healthy Community in Banyumas, Indonesia.Methods: This descriptive observational study enrolled 60 healthcare workers and 60 healthy communities in Banyumas district.  Antibiotic susceptibility test was using disc diffusion according to Clinical laboratory Standard Institute (CLSI) 2019. Biofilm-producing ability identified by using microtiter plate biofilm assay and the positivity of icaAD gene was performed by using PCR method.Results: The results showed that one of 60 healthcare workers (0,017%) showed MRSA, four of 60 healthcare workers (0,07%) were MSSA and 2 samples from community (0,03%) were MSSA. Total of 7 samples underwent biofilm examination,  one sample was moderate biofilm, two samples were weak biofilm, and four samples were no biofilm. It was known that three biofilm-producing S.aureus were positive ica A/D gene.Conclusion: The ica A/D gene was found positive in both biofilm-producing MRSA and MSSA strain from both healthcare workers group and the healthy communities group. The presence of icaAD genes in both strains shows the potential for antibiotic resistance in these strains regulated by different mechanisms.
GFR and Blood Lead Levels in Gas Station Workers Based on δ-Alad Gene Polymorphisms Lantip Rujito; Muhammad Nur Hanief; Paulus Gozali; Joko Mulyanto
Jurnal Ners Vol. 10 No. 1 (2015): April 2015
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (332.002 KB) | DOI: 10.20473/jn.v10i1.1878

Abstract

Introduction: Lead is a well-known toxic agent that makes an organ’s failure. Lead serum itself is infl uenced by δ-ALAD gene polymorphisms (Amino Levulinic Acid Dehydratase). δ-ALAD gene encodes an ALAD enzyme used for heme synthesis. The Characteristic of gene polymorphism may result in Glomerulo Filtration Rate (GFR) value as mark of renal failure. The goal of this study was to fi nd correlations between blood lead levels with GFR in terms of δ ALAD gene polymorphisms.Methods: A cross-sectional design was used to perform this research. Thirty-eight gas stations workers in Banyumas were recruited in this study. δ-ALAD gene polymorphisms were characterized using PCR-RFLP method, while lead serum levels were quantifi ed by Atomic Absorption Spectrophotometer (AAS). In addition, Creatinin serum was done with a spectrophotometer and GFR value was formulated by means of the Schwartz method.Result: The study showed that the proportion of ALAD genotype for ALAD 1-1, 1-2 and 2-2 were 94.7%, 5.3%, and 0% respectively. The mean of serum levels in homozygous 1-1 was 15.94 ppb and heterozygote 1-2 was 1.15 ppb. GFR of participants ranged from 71.11 mL/min to 185.20 mL/min with a mean of 117.34mL/min. There was no correlation between serum Pb and GFR (p = 0.19). Study also could not determine the correlation between GFR and ALAD gene Polymorphism.Conclusion: Study then concluded that there was no correlation between blood lead levels in the GFR on each δ-ALAD genotypes.
Co-Authors A. Haris Budi Widodo Abdul Aziz Agus Suroso Alfi Muntafiah Ali Taqwim Alifah, Assha Luthfianie Amalia Muhaimin Amilia Ramadhani Aminuddin, Muhammad Fahmi Anton Budhi Darmawan Arfi Nurul Hidayah Ariadne Tiara Hapsari Ariadne Tiara Hapsari Ariadne Tri Hapsari Arif Imam Hidayat Arini Dewi Setyowati Assha Luthfianie Basalamah, Muhammad Darmawan, Anton Budhi Desiyani Nani Dhadhang Wahyu Kurniawan Dinar Faiza Diyah Woro Dwi Lestari Dwi Kustiani, Dwi Dwi Utami Anjarwati Dyla Annisa Putri Eman Sutrisna Eman Sutrisna Faiza, Dinar Farah Coutrier Filliana Savitri Fitranto Arjadi Fitranto Arjadi Fitranto Arjadi Fitranto Arjadi Gembong Satria Mahardhika Gembong Satria Mahardhika Hatmoko, Sito Hidayat Sulistyo Ika Murti Harini Ika Ristianingrum, Ika Ina Permata Dewi Indah Pusparini Indah Rahmawati Intani Kurnia Savitri Izzati Muhimmah Joko Mulyanto Joko Mulyanto Joko Mulyanto, Joko Joko Setyono Khafid Nawawi Kotale, Nichola Noelle Krisniawati, Nia Lalu Mutawalli Leily Trianty Lestari , Diyah Woro Dwi Lestari, Diyah Woro Dwi Lies Anne Severijnen, Lies Anne Metta Ayu Susanti Metta Ayu Susanti Miko Ferine Moh Reza Syaifur Rizal Monika Putri Solikah Muhammad Nur Hanief Muhammad Nur Hanief, Muhammad Nur Mujiburrahman, Husnan Nendyah Roestijawati Norina Agatri Nouval Keandre Nur Signa Aini Gumilas Nurcahyani, Putri Silvia Pamela Sandhya De Jaka Paulus Gozali Paulus Gozali, Paulus Peter Hanzon, Peter Pramuditya, Hafizh Zufar Pugud Samodro Purwoko, Ari Qodri Santosa Qodri Santosa, Qodri Rani Afifah Nur Histiyani Rendi Retissu Rintis Noviyanti Rizki Rijatullah Rob Willemsen, Rob Rohmatul Fajriyah Saien Saien Sakina, Ghaida Saryono Saryono Siti Aminah Siti Munfiah Siwi Pramatama Mars Wijayanti SIWI PRAMATAMA MARS WIJAYANTI Sjafril Sanusi Sultana MH Faradz Suprihatin Suprihatin Teguh Haryo Sasongko Thianti Sylviningrum Vitasari Indriani Wahyu Siswandari Wahyudin Wahyudin Wahyudin Wahyudin Wayan Tunas Artama Widodo, Yundandhika Rizki Wijaya, Helmi Aditya Wiwiek R Adawiyah Yudhi Wibowo Zaenal Adi Susanto Zulfa Zahra Salsabila