Garuda - Garba Rujukan Digital

I Roostika, I

Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Author-ID : 586616

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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KRIOPRESERVASI TANAMAN PURWOCENG {Pimpinella pruatjan Molk.) DENGAN TEKNIK VITRIFIKASI Roostika, I; Darwati, I; Megia, R
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Pruatjan (Pimpinellapruatjan Molk.) is an Indonesian endangered medicinal plant that included in Appendix I based on CITES. Therefore it is a highly protected species. To avoid extinction of this plant, it is very important to conserve the plant. In vitro conservation is more suitable since this plant is difficult to be cultivated outside of its habitat. Cryopreservation technique may conserve this material for a long-term period. The objectives of this research were to find optimized treatments for pre culture, loading, and dehydration on cryopreservation of pruatjan. The research was conducted at Tissue Culture Laboratory in Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, started from May to November 2007. Pre culture was conducted using DKW basal media that added by sucrose at the level of 0.3,0.4, and 0.5M for one and three days incubation. Loading was conducted in DKW basal media containing 2M glycerol and 0.4M sucrose for 15,30, and 45 minutes duration time. Dehydration was conducted in several cryoprotectants, namely PVS1 (22% glycerol + 13% propy lene glycol + 13% etylene glycol + 6% DMSO + 3% sucrose), PVS2 (30% glycerol + 15% etylene glycol + 15% DMSO + 0,4M sucrose), PVS3 (50% glycerol + 50% sucrose), and PVS4 (35% glycerol + 20% etylene glycol + sucrose 0.6M). Result showed that pruatjan could be preserved through cryopreservation by vitrification method. The best pre culture was using 0.3 M sucrose for one day, the best loading was 30 minutes, while the best cryoprotectant was PVS2 with 90% success before freezing and 40% after freezing. The success may be improved by applying pre growth treatment, optimizing temperature of thawing, modification of recovery media and incubation condition.

INDUKSI MUTASI DAN SELEKSI IN VITRO MENGGUNAKAN ASAM FUSARAT UNTUK KETAHANAN PENYAKIT LAYU PADAPISANG AMBON HIJAU Lestari, Endang G; Mariska, I; Roostika, I; Kosmiatin, M
BERITA BIOLOGI Vol 8, No 1 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Due to its high vitamin and nutrition content, banana (Musa paradisiaca L.) is deemed necessary as the mineral resources. The demand on the disease free seedlings are recently increasing. However, facing the problems of Fusarium attack in the production centers, health seedlings in the sufficient number are difficult to be provided. Hence, to solve the problems, mutative induction and in vitro selection to the shoot tip explants has been carried out in banana cv "Ambon Hijau", This research was conducted at the tissue culture laboratory of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development. On level of 500; 750; 1000; and 1500 rad, Gamma ray radiation have been applied, continued by in vitro selection by using 0, 30 and 45 mg/1 fusaric acid. The selected explant about 0,5 cm were treated for 2 x 4 week selection period. The result showed that the best medium for regeneration was MS basal medium contains 3 mg/1 BA. The irradiation could increase somaclonal variation as well as created some new somaclones that resistant to fusaric acid. However irradiation and in vitro selection caused inhibition of culture growth. The more dosage of irradiation and concentration of fusaric acid decreased regeneration rate of explant. Inoculation by using conidia (5 g/kg soil) provided 18 putative mutant and higher concentration of conidia (lOg/lOkg soil) produced 37 mutant that resistant to Fusarium.

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