Garuda - Garba Rujukan Digital

Mariska, I

Research Center for Biology-Indonesian Institute of Sciences

Author-ID : 793249

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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INDUKSI KERAGAMAN SOMAKLONAL DENGAN IRADIASI SINAR GAMMA DAN SELEKSI IN VITRO KALUS PISANG RAJABULU MENGGUNAKAN ASAM FUSARAT, SERTA REGENERASI DAN AKLIMATISASI PLANTLET Lestari, Endang G; Purnamaningsih, R; Mariska, I; Hutami, Sri
BERITA BIOLOGI Vol 9, No 4 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Pisang raja bulu is one of the most important bananas in Indonesia. However, this plant has low toleranee to wilt disease, caused by Fusarium oxysporum f. eubense. Its mass cultivation is inhibited by the absence of variety tolerant to the disease.A wide range of genetic variability will be needed if selection for novel characters is to be conducted, especially when there is no source of resistance gene available for breeding materials. This research consisted of callus induction from primary explant, induction of somaclonal variation using gamma iradiation, and in vitro selection using fusaric acid, followed by regeneration and acclimatization of selected plantlets. The media applied for callus induction was MS (Murashige and Skoog. 1962) + 2,4-D I and 3 mg/l + NAA 0 and 0.1 mg/l and 2,4-D 5 mg/l + BA 0.5 mg/l + Casein hidrolysate (CH) 500 mg/l. The applied gamma irradiation dosage were 0, 5.0. 7.5. 10 and 15 Gy. The irradiated calli was subsequently subcultures on selection media i.e.. MS containing fusaric acid at 30 and 45 mg/l. The living calli was then regenerated on media containing BA, TDZ. with or without proline and arginine. In addition. MS+ kinetin 5 mg/l + 1AA 0,2 mg/l was applied for shoot development. The result showed that the most suitable callus induction media for pisang raja bulu was MS +2.4-D 5 mg/l +BA 0.5 mg/l +CH 500 mg/l. The gamma irradiation of 10 Gy produced somaclone lines which were able to proliferate bud nodules on selection media containing fusaric acid at 30 and 45 mg/l. The media used for shoot development was MS + kinetin 5 mg/l + IAA 0,2 mg/l. Planllet obtained from the in vitro were then successfully acclimatized in the green house.

INDUKSI MUTASI DAN SELEKSI IN VITRO MENGGUNAKAN ASAM FUSARAT UNTUK KETAHANAN PENYAKIT LAYU PADAPISANG AMBON HIJAU Lestari, Endang G; Mariska, I; Roostika, I; Kosmiatin, M
BERITA BIOLOGI Vol 8, No 1 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Due to its high vitamin and nutrition content, banana (Musa paradisiaca L.) is deemed necessary as the mineral resources. The demand on the disease free seedlings are recently increasing. However, facing the problems of Fusarium attack in the production centers, health seedlings in the sufficient number are difficult to be provided. Hence, to solve the problems, mutative induction and in vitro selection to the shoot tip explants has been carried out in banana cv "Ambon Hijau", This research was conducted at the tissue culture laboratory of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development. On level of 500; 750; 1000; and 1500 rad, Gamma ray radiation have been applied, continued by in vitro selection by using 0, 30 and 45 mg/1 fusaric acid. The selected explant about 0,5 cm were treated for 2 x 4 week selection period. The result showed that the best medium for regeneration was MS basal medium contains 3 mg/1 BA. The irradiation could increase somaclonal variation as well as created some new somaclones that resistant to fusaric acid. However irradiation and in vitro selection caused inhibition of culture growth. The more dosage of irradiation and concentration of fusaric acid decreased regeneration rate of explant. Inoculation by using conidia (5 g/kg soil) provided 18 putative mutant and higher concentration of conidia (lOg/lOkg soil) produced 37 mutant that resistant to Fusarium.

PENINGKATAN KERAGAMAN GENETIK MAWAR MINI MELALUI KULTURIN-VITRO DAN IRADIASI SINAR GAMMA Handayati, Wahyu; Darliah, Darliah; Mariska, I; Purnamaningsih, R
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Mini rose (Rosa hybrida L.)is one of the favorite ornamental plants. To get a new appearance of this mini rose, two experiments were conducted at Cipanas and Bogor, from April 1999 to March 2000.In the first experiment, the treatment was the dosage of gamma ray irradiation, i.e. 0, 1, 2, 3, 4, 6, 8, 10 and 12 krad.In the second experiment, the treatment was the concentration of callus induced mediumi.e. 2,4-dichlorophenoxyacetic acid (2,4-D) (0.5 and 1 mg/1) + chinetine (1, 3 and 5 mg/1).Randomized Block Design was used with 5 replications. Mini rose Romantica Meilandina (pink color) and Prince Meilandina (dark red color) was used as a source of plant material.The results showed that the irradiation dosage from 1 until 3 krad gave the best appearance compared with the other treatments.Considering the observance to plantlet in the same dosage, the color of the flower was changed from the natural color to white and red color.The combination of 0,5 mg/12,4- dichlorophenoxyacetic acid + 3 mg/1 chinetine was the best medium to the callus growth.

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