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All Journal Planta Tropika Buletin Penelitian Tanaman Rempah dan Obat
Nursalam Sirait, Nursalam
Balai Penelitian Tanaman Rempah dan Obat
Agriculture, Biological Sciences & Forestry

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MULTIPLIKASI TUNAS, PERAKARAN DAN AKLIMATISASI TANAMAN SAMBANG NYAWA (Gynura procumbens) Kristina, N. Nova; Sirait, Nursalam; Bermawie, Nurliani
Buletin Penelitian Tanaman Rempah dan Obat Vol 16, No 2 (2005): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v16n2.2005.%p

Abstract

Shoots Multiplication, Rooting, and Acclimatization of Gynura procumbensThe research was performance to obtain shoots multiplication, rooting and acclimati-zation of Gynura procumbens was conducted    January  2004 to May 2005 at the Laboratory of Tissue Culture of Gremplasm and Breeding Division. This research was conducted within two steps, i.e. 1) : Shoots multiplication  in : MS + BA (0; 0,1; 0,3 and 0,5 ) mg/l; 2) rooting and acclimatization. Explants were culture on rooting medium MS + IAA (0,1; 0,3); MS + IBA  (0,1; 0,3) or NAA (0,1 and 0,3) mg/l. Acclimatization were performanced on the two kinds of media i.e. dung manure + soil (1 : 1) or husk + soil (1 : 1). Rooting and shoots multiplication were arranged in completely randomized design, with 10 replications and 2 explants for each bottle. Acclimatization was arranged in randomized-block design with 10 replications and 1 plantlet for each treatment. The results showed the best medium for multiplication shoot was MS-free hormone with 5,4 shoots, 2 months after cultured. The highest number of roots was obtained in NAA 0,1 mg/l with 9,3/plantet. MS + IBA 0,3 mg/l give the longest roots (9,58 cm) and IAA 0,1 mg/l the highest number of leaf (12/plantet). Interaction between the source medium and acclimatization medium was observed however, there was no significantly difference between IAA 0,1 mg/l and IBA 0,1 mg/l in number of shoots and long shoots (5,2 and 5,01 cm). 
Floral Morphological Variation in Black Pepper (Piper nigrum L.) Varieties and Hybrid Lines Wahyuni, Sri; Susilowati, Mariana; Sirait, Nursalam; Bermawie, Nurliani
PLANTA TROPIKA Vol 12, No 2 (2024)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v12i2.15970

Abstract

The inter-variety crossing is a promising approach to increase black pepper production. However, successful hybridization hinges on floral characteristics. This study investigated the floral morphology of diverse pepper varieties and their hybrid offspring. Eighteen genotypes, including parent varieties and F1 hybrids, were cultivated in pots under greenhouse conditions at the Indonesian Spice and Medicinal Crops Research Institute, Bogor. Flower and fruit traits were observed.  Results indicated variability in spike morphology. While most ripe spikes were yellowish-green, exceptions included LH 20-1 and LH 22-1. Ciinten and LH 4-5 displayed superior spike length and fruit set, contrasting with the shorter, less productive spikes of 20-1, 22-1, and 455-N2-97.  Stigma receptivity and another dehiscence coincided in Ciinten, N2BK, LH 6-2, and LH 4-5, simplifying the hybridization process. Conversely, earlier stigma receptivity in Petaling 1, Petaling 2, Natar 1, Natar 2, LH 44-9, LH 20-1, and LH 22-1 facilitated castration procedures. These findings offer valuable insights for optimizing pepper breeding programs.
Co-Authors Kristina, N. Nova Mariana Susilowati, Mariana Nurliani Bermawie Sri Wahyuni