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All Journal Vegetalika Planta Tropika Makara Journal of Science
Mariana Susilowati, Mariana
Jurusan Budidaya Pertanian, Fakultas Pertanian, Universitas Gadjah Mada, Bulaksumur, Yogyakarta
Agriculture, Biological Sciences & Forestry

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Identification of A Major Quantitative Trait Locus for Grain Weight In Rice Using Microsatellite Marker Susilowati, Mariana; Aswidinnoor, Hajrial; Enggarini, Wening; Trijatmiko, Kurniawan Rudi
Makara Journal of Science Vol. 21, No. 4
Publisher : UI Scholars Hub

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Abstract

Rice is one of the major staple foods in the world, especially in Asia. Improving yield potential of superior cultivars is important to meeting the demand for rice production, which is increasing due to human population increase, climate change, and degradation of agricultural resources, such as land and water. In this study, a BC3F2 population developed from an intraspecific cross between Ciherang and a new plant type line (B11143D) was used in a quantitative trait locus (QTL) analysis. Ciherang is a high yielding rice cultivar with good grain quality which has been planted in 37% of the irrigated rice area in Indonesia. The objective of this study was to identify QTL(s) for yield components on chromosome 12, which can be used to improve the elite cultivar Ciherang or other popular cultivars through marker-assisted breeding. A total of two hundred BC3F2 lines were evaluated in the greenhouse during this study. The population was observed for eight agronomic traits including days to heading (dth), plant height (ph), flag leaf length (fll), panicles per plant (ppl), panicle length (pl), grains per panicle (gpp), 1000-grain weight (gw), and yield (yld). Four simple sequence repeats (SSR) markers (RM3472, RM28048, RM28195, and RM1986) were used for targeted mapping on chromosome 12. Linkage analysis identified a QTL for 1000-grain weight located on chromosome 12 at position 53.5 cM–73 cM.
Floral Morphological Variation in Black Pepper (Piper nigrum L.) Varieties and Hybrid Lines Wahyuni, Sri; Susilowati, Mariana; Sirait, Nursalam; Bermawie, Nurliani
PLANTA TROPIKA Vol 12, No 2 (2024)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v12i2.15970

Abstract

The inter-variety crossing is a promising approach to increase black pepper production. However, successful hybridization hinges on floral characteristics. This study investigated the floral morphology of diverse pepper varieties and their hybrid offspring. Eighteen genotypes, including parent varieties and F1 hybrids, were cultivated in pots under greenhouse conditions at the Indonesian Spice and Medicinal Crops Research Institute, Bogor. Flower and fruit traits were observed.  Results indicated variability in spike morphology. While most ripe spikes were yellowish-green, exceptions included LH 20-1 and LH 22-1. Ciinten and LH 4-5 displayed superior spike length and fruit set, contrasting with the shorter, less productive spikes of 20-1, 22-1, and 455-N2-97.  Stigma receptivity and another dehiscence coincided in Ciinten, N2BK, LH 6-2, and LH 4-5, simplifying the hybridization process. Conversely, earlier stigma receptivity in Petaling 1, Petaling 2, Natar 1, Natar 2, LH 44-9, LH 20-1, and LH 22-1 facilitated castration procedures. These findings offer valuable insights for optimizing pepper breeding programs.
Peningkatan Keragaman Genetik Tanaman Monstera adansonii melalui Induksi Mutasi Kimia dengan Streptomycin Susilowati, Mariana; Aisyah, Syarifah Iis; Rapi, Muh. Nur Alif; Kartiman, Roni
Vegetalika Vol 13, No 2 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.81993

Abstract

Monstera adansonii variegata merupakan salah satu jenis tanaman hias daun yang popular di masyarakat sejak pandemi Covid-19. Variegata adalah perbedaan warna dan corak daun dengan tanaman normal yang diperoleh dari mutasi. Mutasi pada M. adansonii dapat dilakukan dengan berbagai mutagen kimia salah satunya streptomycin. Penelitian ini bertujuan mengetahui konsentrasi dan lama perendaman streptomycin yang optimum untuk menghasilkan tanaman M. adansonii variegata. Penelitian ini dilaksanakan selama 4 bulan di Kebun Percobaan IPB Sukamantri menggunakan Rancangan Kelompok Lengkap Teracak (RKLT) faktorial. Hasil penelitian menunjukkan bahwa interaksi antara konsentrasi streptomycin sebesar 750 ppm dengan lama perendaman 48 jam menunjukkan hasil yang paling baik diantara kombinasi perlakuan lainnya dengan rata-rata persentase mutan putatif sebesar 73,33%. Hasil uji lanjut menggunakan kontras polynomial menunjukkan bahwa konsentrasi dan lama perendaman streptomycin paling optimum untuk menghasilkan M. adansonii variegata adalah konsentrasi 860 ppm dengan lama perendaman 81,64 jam. Perubahan warna dan pola daun pada mutan putatif ini akan meningkatkan nilai ekonomi. Sementara itu antibiotik streptomycin juga mampu menghambat pertumbuhan dan perkembangan M. adansonii seperti tinggi tanaman, diameter batang, jumlah daun, dan luas daun.
Co-Authors HAJRIAL ASWIDINNOOR Kartiman, Roni Kurniawan Rudi Trijatmiko, Kurniawan Rudi Nurliani Bermawie Nursalam Sirait, Nursalam Rapi, Muh. Nur Alif Sri Wahyuni Syarifah Iis Aisyah Wening Enggarini