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All Journal Bioscientist : Jurnal Ilmiah Biologi
Akhmad Sukri
Program Studi Pendidikan Biologi, FSTT, Universitas Pendidikan Mandalika
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences

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Karakteristik Morfologi dan Analisis Fenetik Kerbau (Bubalus bubalis) Nusa Tenggara Barat Akhmad Sukri
Bioscientist : Jurnal Ilmiah Biologi Vol 9, No 2 (2021): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v9i2.7897

Abstract

Measurement of morphological characteristics is needed to understand buffalo's conformation, productivity and reproduction. This study aims to describe the morphological characteristics of buffalo taken from the West Nusa Tenggara region and to carry out phenetic analysis based on the results of morphological character measurements. This study was exploratory with 16 individual buffalo as subjects taken purposively from two different populations, namely West Sumbawa and West Lombok Regencies, West Nusa Tenggara. The morphological characters measured included leg length, hip width and head width. Morphological character data were analyzed descriptively and non-parametrically using the Mann-Whitney test. In contrast,the phenetic analysis used the Unweighted Pair Group Mean Average (UPGMA) method supplemented by Principle Component Analysis (PCA). The measurement results showed variations in the morphology of the buffalo (Bubalus bubalis) in West Sumbawa and West Lombok Regencies. The character of leg length was significantly different in both populations (Sig <.05). In contrast, the other two characters, namely head width and hip height, were not significantly different (Sig >.05). Furthermore, the phenetic analysis showed that the relationship between the two populations of buffalo was significantly different, which was strengthened through PCA analysis which revealed that there was no genetic mixing between the buffalo populations.
Studi Penggunaan Safranin dan Kolkisin dalam Pengamatan Kromosom pada Sel Akar Bawang Bombay (Allium cepa Var) Noval Febriadi; Nofisulastri Nofisulastri; Akhmad Sukri
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 1 (2023): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i1.7255

Abstract

Based on the results of initial observations it was found that the use of safranin and colchicine showed less than optimal results. The purpose of this study was to observe the phases of mitotic division seen in the use of safranin and colchicine in onion (Allium cepa Var) root cells. The stages of the research included the stages of making preparations, namely root growth, root cutting, fixation, maceration, staining, and squashing. and continued with the observation of the phase of mitotic division which appeared microscopically. The data obtained were from the results of observing the cleavage phase in the chromosomes of the onion root cells, then analyzed descriptively and then comparatively to show what cleavage phases were due to safranin and colchicine staining with different soaking times. Based on the observed cell division phase of onion (Allium cepa Var) roots, safranin was more effective than colchicine with 30 minutes of soaking time.
Co-Authors Nofisulastri Nofisulastri Noval Febriadi