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All Journal Bioscientist : Jurnal Ilmiah Biologi
Nofisulastri Nofisulastri
Program Studi Pendidikan Biologi, FSTT, Universitas Pendidikan Mandalika
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences

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INVENTARISASI SERANGGA TANAH DI TAMAN WISATA ALAM GUNUNG TUNAK KABUPATEN LOMBOK TENGAH Uswatun Hasanah; Nofisulastri Nofisulastri; Safnowandi Safnowandi
Bioscientist : Jurnal Ilmiah Biologi Vol 8, No 1 (2020): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bjib.v8i1.2560

Abstract

Gunung Tunak Nature Tourism Park is a nature conservation area which is mainly used for tourism and nature recreation. Gunung Tunak Nature Tourism Park is one of 17 conservation areas managed by the Natural Resources Conservation Center (BKSDA), located in Mertak Village, Pujut District, Central Lombok Regency. Geographically located between 08º53'30.08º57'30 South Latitude, and 116º24'00” East Longitude. The purpose of this study was to determine the types of soil insects found in the Gunung Tunak Nature Park. This type of research is exploratory descriptive. The sampling method uses pitfall traps. Sampling locations include the park area in front of the butterfly breeding, and in front of the camping ground as posts 1 and 2. Sampling was carried out in the morning (07.00-09.30 WITA), and in the afternoon (16.00-17.30 WITA) with 3 repetitions. Soil insect samples obtained were identified at the level of the order and family at the Biology Laboratory, Mandalika University of Education. The results showed that 6 soil orders were found, namely: Orthoptera, Hymenoptera, Hemiptera, Diptera, Coleoptera, and Scorviones. The highest group of individuals found was the Hymenoptera Order with 93 individuals.
Deteksi Cacing Capillaria hepatica pada Organ Hati Tikus Supriadi Supriadi; Dina Oktaviana; Nofisulastri Nofisulastri
Bioscientist : Jurnal Ilmiah Biologi Vol 9, No 2 (2021): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v9i2.4405

Abstract

Capillaria hepatica is a zoonotic parasitic agent that has been reported in various parts of the world. The presence of C. hepatica in an area is closely related to the presence of a rat population. Considering the life cycle of C. hepatica which is easily transmitted to humans, one of the most vulnerable places for transmission of C. hepatica to humans is the market area. This study aims to detect the presence of C. hepatica worms in the rat population in Public Market Kebon Roek, Ampenan District, Mataram City. This type of research is descriptive research with a cross sectional study design (cross-sectional study). To determine the number of samples, an accidental study approach was used, namely the number of rat samples used was the number of rats caught during the study period (set for 2 traps). 10 samples of rat liver were collected. The liver was then preserved in 10% formalin solution at the Microanatomy Laboratory, Department of Anatomy, Faculty of Veterinary Medicine, Gadjah Mada University. Meanwhile, histology preparations and GI fluid examination were carried out at the Clinical Skill Center (CSC) Laboratory, Faculty of Veterinary Medicine, Mandalika University of Education. Data in the form of images of worm eggs and histopathology of liver tissue were analyzed descriptively and presented in the form of figures and tables. The results of the examination of 10 samples of rat liver histology preparations showed that 4 positive samples contained eggs and adult worms of C. hepatica. Where the 3 histological preparations observed showed that the rat liver was severely damaged, and 1 sample appeared to have minor damage. For examination of gastrointestinal fluids, there were no samples containing C. hepatica worm eggs.
Keragaman Ekspresi Lokus Gen Kucing Peranakan Anggora Hasil Perkawinan Silang Alami Nofisulastri Nofisulastri; Supriadi Supriadi
Bioscientist : Jurnal Ilmiah Biologi Vol 9, No 1 (2021): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bjib.v9i1.3734

Abstract

This research is a follow-up study by presenting the extent of the expression pattern and the presence of each allele locus that marks the color pattern on the cat's body, to add a reference in making it easier for the cat breeder community to form relatively uniform color patterns in certain crossing periods. This research is a descriptive exploratory case study presented qualitatively. Cat samples were screened based on a minimum age of 1 year, and 30 individuals were collected by road sampling. Observation of sample morphology based on mating pathway, recording of morphological characters (body color, color pattern, and tail length). Data were analyzed using allele frequency calculations for genes (dominant and recessive relationship) and concluded qualitatively. The frequency of the presence of gene loci and their alleles was found as many as 8 gene loci, namely: w-W; A A; B-b; C_cb_cs_ca_c; Ta_T_tb; D-d; s-S; OO-oo-Oo; and M-m, and no D-d and I-i gene loci were found. It was concluded that the observed gene locus expression of the Angora crossbreed cat had a variety of random allele frequencies, and did not indicate any particular allele dominance.
Kualitas Peer Assessment sebagai Assessment Formatif Siti Rabiatul Adawiyah; Nofisulastri Nofisulastri
Bioscientist : Jurnal Ilmiah Biologi Vol 8, No 2 (2020): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bjib.v8i2.3159

Abstract

This study aims to produce a suitable peer assessment instrument as a formative assessment. This type of research is development research, because it develops a peer assessment instrument. The development of the peer assessment instrument refers to the Borg & Gall development model which includes 10 stages, namely: 1) a preliminary study; 2) planning; 3) development of a product initiation model; 4) reviewing the initiation model of the product; 5) revision; 6) limited trials; 7) revised trial results; 8) wide-scale trials; 9) final product revision; and 10) dissemination. However, in this study only up to stage 8, while stage 9 and stage 10 were not carried out due to time and conditions that did not allow. Expert validation was carried out beforehand before limited testing and wide-scale trials in Class X Madrasah Aliyah An-Najah, Al-Halimy Education Foundation, Sesela, Gunungsari District, West Lombok Regency. The results showed that the logical validity of the peer assessment instrument was very good, with an average score of 3.7. The results of the analysis using the product moment correlation test to determine the empirical validity of the peer assessment showed that the empirical validity of the peer assessment was in the high category (0.94; 0.96; 0.97; and 0.97). Based on the results of the study, it was concluded that peer assessment was of good quality as a formative assessment.
Studi Penggunaan Safranin dan Kolkisin dalam Pengamatan Kromosom pada Sel Akar Bawang Bombay (Allium cepa Var) Noval Febriadi; Nofisulastri Nofisulastri; Akhmad Sukri
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 1 (2023): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i1.7255

Abstract

Based on the results of initial observations it was found that the use of safranin and colchicine showed less than optimal results. The purpose of this study was to observe the phases of mitotic division seen in the use of safranin and colchicine in onion (Allium cepa Var) root cells. The stages of the research included the stages of making preparations, namely root growth, root cutting, fixation, maceration, staining, and squashing. and continued with the observation of the phase of mitotic division which appeared microscopically. The data obtained were from the results of observing the cleavage phase in the chromosomes of the onion root cells, then analyzed descriptively and then comparatively to show what cleavage phases were due to safranin and colchicine staining with different soaking times. Based on the observed cell division phase of onion (Allium cepa Var) roots, safranin was more effective than colchicine with 30 minutes of soaking time.
Co-Authors Akhmad Sukri Dina Oktaviana Noval Febriadi Safnowandi Safnowandi Siti Rabiatul Adawiyah Supriadi Supriadi Uswatun Hasanah