Article Per Year (5 Year)
p-Index From 2020 - 2025
0.92
P-Index
This Author published in this journals
All Journal JURNAL KIMIA SAINS DAN APLIKASI
Mukhammad Asy'ari
Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Jl. Prof. Soedarto, SH., Tembalang, Semarang|Diponegoro University|Indonesia
Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering

Published : 5 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search

 1 
Effect of Storage of Yellow Pigment from Halophilic Bacillus clausii J1G-0%B on Antioxidant Activity Galuh Dwi Arum; Mukhammad Asy'ari; Nies Suci Mulyani
Jurnal Kimia Sains dan Aplikasi Vol 25, No 11 (2022): Volume 25 Issue 11 Year 2022
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.25.11.399-404

Abstract

Oxidative stress is a state of excess free radicals in the body, which results in increased oxidation processes in the body’s cells and causes damage. In previous studies, one way to neutralize this situation was found, namely with halophilic bacteria. Halophilic bacteria can live at a salt content of 2% to 30%. These bacteria can produce various kinds of pigments for self-defense from extreme environments, which function as immunomodulatory compounds and have antioxidant activity. The antioxidant activity of the yellow pigment halophilic bacteria Bacillus clausii J1G-0%B using the DPPH method showed that the effect of light and air on antioxidant activity was 17.88% inhibition in the crude extract and 14.24% inhibition at 1600 ppm. Antioxidant activity decreased by 17.13% under the influence of air, 1.89% under the influence of light, and 28.58% under the influence of air and light. FTIR analysis under the influence of air experienced a decrease in the peak of the CO carbonyl group of 1739.29 cm-1 and an increase in the alkane C-H group of 1369.52 cm-1, while under the influence of light and open conditions, the carbonyl CO group experienced an increase of 1739.50 cm-1 and 1739.46 cm-1 and the increase in alkane C-H groups 1371.41 cm-1 and 1369.53 cm- 1.
Interaction Mechanism of Inhibition of Palmitic Acid and α Selinene Targeting FabH and FabI Enzymes in Escherichia coli: In Silico Study Tia Aulia; Nies Suci Suci Mulyani; Mukhammad Asy'ari
Jurnal Kimia Sains dan Aplikasi Vol 25, No 12 (2022): Volume 25 Issue 12 Year 2022
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.25.12.427-435

Abstract

Investigation studies of the interaction mechanism of palmitic acid and α-selinene in inhibiting FabH and FabI enzymes have been studied using an in silico approach. FabH (Beta-Ketoacyl-ACP Synthase III) and FabI (Enoyl-acyl carrier protein reductase) enzymes are two enzymes that are targets for the inhibition of candidate antibacterial compounds. This study aimed to determine the strongest candidate between palmitic acid and α-selinene as an antibacterial agent for Escherichia coli. The method used in this study is a random and directed molecular docking method using the Autodock Vina program, which is integrated into PyRx 0.8 software. The results of the molecular docking simulation include the pattern and strength of interaction between the ligand and the FabI and FabH enzymes. The interaction pattern includes the cluster pattern, the ligand poses on the protein surface, and the interaction strength based on the binding affinity value. Based on the results of random docking simulation data analysis, it was shown that the majority of α-selinene occupied the position of cluster 1 of the FabI enzyme and palmitic acid in cluster 2 of the FabH enzyme. Based on the binding affinity value, palmitic acid has a weaker interaction strength on the FabH enzyme (-5.7 kcal/mol) than on the FabI enzyme (-7.1 kcal/mol). The interaction strength of α-selinene on the FabI enzyme (-7.3 kcal/mol) was stronger than that of the FabH enzyme (-6.9 kcal/mol). The interaction strength of α-selinene in both FabI and FabH enzymes was greater than that of palmitic acid. α-selinene is projected to have a better potential as an antibacterial agent against Escherichia coli than palmitic acid based on its greater interaction strength.
Characterization of a Halostable Metalloprotease from the Halophilic Bacterium Bacillus clausii J1G-0%B Annisa Dila Febriyanti; Mukhammad Asy'ari
Jurnal Kimia Sains dan Aplikasi Vol 27, No 8 (2024): Volume 27 Issue 8 Year 2024
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.27.8.395-402

Abstract

Protein plays a crucial role as a biocatalyst in various industries, particularly in breaking down proteins into amino acids. The demand for proteases capable of functioning under extreme conditions, such as high salinity, temperature, and pH, is increasing. To address this, the exploration of bacteria that produce stable proteases in such environments is essential. Bacillus clausii J1G-0%B, a halophilic bacterium isolated from Madura salt ponds, thrives in salinity levels of 0-20% NaCl. This study aims to obtain and characterize the protease produced by Bacillus clausii J1G-0%B, focusing on its activity and stability under extreme conditions. The research involved screening, production, and purification of the protease using ammonium sulfate fractionation and dialysis. Protease activity was measured using the Kunitz method, and protein content was determined using the Lowry method. Characterization included optimizing enzymatic conditions (pH, temperature, NaCl concentration), identifying metalloprotease types, and analyzing enzyme kinetics and thermodynamics. The study successfully produced protease using a halophilic medium with casein and 5% NaCl. After 96 hours of incubation, the protease exhibited a specific activity of 654.737 U/mg. Optimal activity was observed at pH 7, 50°C, and 10% NaCl, with stability between 2.5% and 15% NaCl concentration. Enzyme kinetics revealed a high affinity for casein, with a KM value of 0.164 mg/mL and Vmax of 13.182 µmol/mL·min. Thermodynamic analysis indicated high stability, as shown by a positive ΔGi value (+105.84 kJ/mol), a low inactivation constant (ki = 0.0031 min-1), and a long half-life (t½ = 223.548 minutes). EDTA chelation tests confirmed that the protease is a metalloprotease. The halostable protease from Bacillus clausii J1G-0%B shows significant potential for industrial applications and bioremediation in high-salinity environments.
Enhanced Antibacterial Efficacy of Ag(I), Cu(II), and Zn(II) Modified Sodalite Zeolite Against Escherichia coli and Staphylococcus aureus Sriatun Sriatun; Khairini Pertiwi; Choiril Azmiyawati; Mukhammad Asy'ari; Damar Nurwahyu Bima; Nor Aida Zubir
Jurnal Kimia Sains dan Aplikasi Vol 27, No 10 (2024): Volume 27 Issue 10 Year 2024
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.27.10.477-484

Abstract

Sodalite zeolite modified with metal ions Ag+, Cu2+, Cu2+, and Zn2+ was successfully synthesized and evaluated for antibacterial activity. The research aims to obtain silver, copper, and zinc metal-modified sodalite separately and determine their antibacterial activity on Escherichia coli and Staphylococcus aureus bacteria. Sodalite zeolite was synthesized using ludox and sodium aluminate through hydrothermal methods, ensuring uniform crystal growth and optimal crystallinity, as confirmed by X-ray diffraction (XRD) analysis. The average particle sizes of the modified zeolites were determined to be 54.9 nm for Ag-Zeolite, 37.2 nm for Cu-Zeolite, and 28.56 nm for Zn-Zeolite, with structural changes observed through alterations in peak intensity. Scanning Electron Microscopy - Energy Dispersive X-ray (SEM-EDX) analysis showed no significant change in the zeolite’s morphology. In addition, the EDX results showed the presence of Ag (3.15%), Cu (3%), and Zn (2.41%) metals indicating successful ion exchange. Antibacterial assays revealed that Cu-Zeolite demonstrated superior efficacy inhibition zones against Escherichia coli (14.04±1.26) and Staphylococcus aureus (20.74±0.48), highlighting its potential as an antimicrobial agent. The mechanism of action involved the controlled release of metal ions, disrupting bacterial cell membranes and metabolic processes. Notably, Cu2+ ions exhibited the strongest antibacterial properties due to their smaller ionic radius and higher electronegativity than Ag+ and Zn2+. This research underscores the promising applications of metal-ion-modified sodalite zeolite in medical and environmental contexts.
An Investigation into the Anti-Aggregation Potential of Swietenia macrophylla Triterpenoid on Bovine Serum Albumin: Docking and RMSF Bayu Fajriansyah Al-Khairi; Mukhammad Asy'ari; Agustina L. N. Aminin
Jurnal Kimia Sains dan Aplikasi Vol 27, No 12 (2024): Volume 27 Issue 12 Year 2024
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.27.12.560-568

Abstract

Protein aggregation, caused by environmental factors, can lead to neurodegenerative diseases. Hydrophobic compounds like latrepirdine are used in medical treatments like anti-Parkinson’s and Huntington’s diseases. Swietenia macrophylla contains abundant hydrophobic compounds from the triterpenoid group, but their anti-aggregation potential has not been reported. This study investigates the hydrophobic interactions and anti-aggregation potential of triterpenoid compounds, including swietenine, swietenolide, khayasin T, beta-sitosterol, and stigmasterol, against bovine serum albumin (BSA). Latrepirdine is employed as the control compound. In silico methods, molecular docking and molecular dynamics showed potential in clusters 1 and 2, with swietenine having a more stable RMSF value than latrepirdine. The study found four clusters with all ligands, with cluster 1 being the earliest protein opening area. Mahogany seed triterpenoid compounds have potential in cluster 1 (51-67%), while cluster 2 has 37-46%. In cluster 2, they have an advantage over latrepirdine (2%). Stigmasterol and beta-sitosterol are spread across the clusters. The swietenine compound has a more stable RMSF value than latrepirdine. This suggests that mahogany seed triterpenoid compounds have potential as anti-aggregation agents.
Co-Authors Agustina L. N. Aminin Annisa Dila Febriyanti Bayu Fajriansyah Al-Khairi Choiril Azmiyawati Damar Nurwahyu Bima Galuh Dwi Arum Khairini Pertiwi Nies Suci Mulyani Nies Suci Suci Mulyani Nor Aida Zubir Sriatun Sriatun Tia Aulia