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Ateng Supriyatna
Universitas Islam Negeri SunanGunung Djati Bandung
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INDUKSI TUNAS ANGGREK (Dendrobium sp) Var. Kumala MENGGUNAKAN BAP (6-Benzyl Amino Purine) DAN AIR KELAPA SECARA IN-VITRO Reni Annisa Nurhanifah; Ateng Supriyatna; Ayuni Adawiyah
Gunung Djati Conference Series Vol. 6 (2021): Seminar Nasional Biologi (SEMABIO) 6 Tahun 2021
Publisher : UIN Sunan Gunung Djati Bandung

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Abstract

Orchid plants are one type of ornamental plant that has a unique flower beauty and a long flower life compared to other flower plants. The beauty and attractiveness of orchids lies in the various shapes and colors of their flowers. because this Dendrobium orchid has the potential as an ornamental plant so that it is widely developed and propagated in Indonesia because the climate is very suitable for growing orchids. This study aimed to determine the effect of BAP (6-Benzyl amino purine) and coconut water on the growth of orchid explants in vitro and to determine the optimum concentration of the combination of BAP and coconut water on the growth of orchid explants in vitro. The method used is the Completely Randomized Design (CRD) method using MS media, there are 10 concentration variations where 1 control concentration and 9 concentrations of BAP and coconut water combination. Each treatment consisted of 3 repetitions. Thus, 30 experimental units were obtained. The results showed that MS media using a combination of 1.5 ppm BAP and 200 ml of coconut water could trigger the growth of leaves, 0.5 ppm BAP treatment plus 150 ml of coconut water could trigger stem elongation and shoot number, while the control treatment can trigger the growth of the number of roots. The survival percentage is 100% where none of the 30 ripening bottles is contaminated.
PENGARUH KONSENTRASI AIR KELAPA (Cocos nucifera L.) TERHADAP EKSPLAN KRISAN (Chrysanthemum morifolium) KULTIVAR ‘XANNE AGRIHORTI’ SECARA IN VITRO Sri Fitri Solihah; Ateng Supriyatna; Ayuni Adawiyah
Gunung Djati Conference Series Vol. 6 (2021): Seminar Nasional Biologi (SEMABIO) 6 Tahun 2021
Publisher : UIN Sunan Gunung Djati Bandung

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Abstract

Krisan (Chrysanthemum morifolium) Cultivars ‘Xanne Agrihorti’ is one of the most popular ornamental plants in Indonesia. One of the problems that arise in the cultivation of chrysanthemums is the high cost of growth regulators. Coconut water contains growth regulators in the form of high cytokinins. The purpose of this study was to determine the effect of coconut water on the growth of krisan (Chrysanthemum morifolium) Cultivars ‘Xanne Agrihorti’ in vitro and determine the optimal concentration of coconut water in the growth of chrysanthemum plants in vitro. This study used a Completely Randomized Design (CDR), with concentration of coconut water which consisted of 4 treatment levels : 0 ml/liter, 100 ml/liter, 150 ml/liter, 200ml/liter. The experiment was carried out 3 times, so the experimental units were 12. Parameters measured were number of shoots, number of roots, stem height, root length and survival rate. Data analysis used the Kruskal-Wallis test. The data obtained after being analyzed statistically through the Kruskal-Wallis test at a confidence level of p<0,05. The results showed that the addition of coconut water with a coconut water had an effect on the growth and development of tissue culture with a concentration of 150 ml/L gave optimal results for the formation of the number of leaves as many as 11 leaves, concentration of 100 ml/L gave the best results for the formation of the number of roots as much as 10 roots, and the percentage of survival was 100%.
Co-Authors Adawiyah, Ayuni Reni Annisa Nurhanifah Sri Fitri Solihah