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All Journal PHARMACOLOGY, MEDICAL REPORTS, ORTHOPEDIC, AND ILLNESS DETAILS (COMORBID) Frontiers on Healthcare Research
Raymond, Benni
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Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience Nursing Public Health

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Journal : Frontiers on Healthcare Research

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Growth Factor Identification Based on Speed and Duration of Centrifugation in Platelet Rich Plasma Rahmadian, Rizki; Raymond, Benni; Meksiko, Riki
Frontiers on Healthcare Research Vol. 1 No. 1 (2024)
Publisher : Rumah Sakit Umum Pusat (RSUP) Dr. M. Djamil

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63918/fhr.v1.n1.p69-75.2024

Abstract

Background: This study aims to determine the appropriate preparation of simple PRP protocol to yield the maximum growth factor concentration, specifically for PDGF and TGF-β1. Methods: Blood samples were collected from 5 healthy volunteers who signed informed consent for participation in the study. The samples then processed by single centrifugation at 4 four different speed (600, 800, 1000, and 1200 rpm) for 4 different centrifugation times ( 8, 10, 12, 14 minutes). The Platelet Derived Growth Factor (PDGF) and Transforming Growth Factor β1 (TGF- β1) concentrations were determined by enzyme-linked immunosorbent assay (ELISA). Blood routine test analysis were measured by hematology analyzer. Results: The 8 min with 600 rpm centrifugation protocol resulted in a slightly greater release of TGF-β1 (7,127 ng/mL) and PDGF (473,909 pg/mL). While the other samples resulted from 50,296 pg/mL (14 min with 1200 rpm) until 406.883 pg/mL (8 min with 800 rpm) of PDGF. The other results of TGF-β1 vary from 0,558 ng/mL (14 min with 1200 rpm) to 6,322 ng/mL (10 min with 600 rpm). Conclusion: The highest concentration of PDGF and TGF-β1 were obtained from centrifugation process at 600 rpm for 8 minutes. Meanwhile, the lowest concentration of PDGF and TGF-β1 were obtained from centrifugation process at 14 min with 1200 rpm.
Histological Comparison of Lyophilized Amniotic Membrane, Tulle Gras Dressing and Topical Gentamicin on Acute Partial Thickness Wound : In Vivo Study Raymond, Benni; Fadila, Srigunda Arisya; Rahmadian, Rizki; Tofrizal, Tofrizal
Frontiers on Healthcare Research Vol. 1 No. 2 (2024)
Publisher : Rumah Sakit Umum Pusat (RSUP) Dr. M. Djamil

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63918/fhr.v1.n2.p1-7.2024

Abstract

Background: Partial thickness wounds refer to the damage that extends from the epidermis to the upper layers of dermis. This study aims to compare the histological regrowth of acute partial thickness wounds following application of lyophilized amniotic membrane, tulle grass dressing and topical gentamicin. This study aims to establish a standard approach for treating partial thickness wound. Methods: Three groups of Mus Musculus mice were used in our experimental study. Similar to harvesting split thickness skin grafts, partial thickness wounds are performed. The wound was then treated with lyophilized amniotic membrane, tulle grass dressing and topical gentamicin. Using the Scoring System for Histological Assessment of Wound Healing, the wound is histopathologically examined at day 14. In this study, One way Anova was used for statistical analysis. Results: Application of lyophilized amniotic membrane showed different histological characteristic of healing from other method, with p=0.001 it showed significance epidermal growth compared to topical gentamicin. Whereas dermal thickness were also better, accompanied by lower dermal collagen density. Amniotic membrane has a large source of stem cells and contains several growth factors which is important in the physiological process of wound healing and tissue regeneration. Conclusions: Characteristic of skin healing using lyophilized amniotic membrane showed rapid epidermal growth and gave impression of less fibrosis tissue, so that would be potential for better wound treatment especially for prevention of scar.
Co-Authors Al Hafiz, Al Hafiz Fadila, Srigunda Arisya Meksiko, Riki Rahmadian, Rizki Supriyadi, Fauzia Latifah Tofrizal