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THE POTENTIAL OF INDIGENOUS BACTERIA FOR REMOVING CADMIUM FROM INDUSTRIAL WASTEWATER IN LAWANG, EAST JAVA Pambudiono, Agung; Suarsini, Endang; Amin, Mohamad
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (688.873 KB) | DOI: 10.11594/jtls.08.01.11

Abstract

Heavy metals have been used in various areas around the world especially in the industrial sector. Heavy metals contamination is very dangerous for ecosystem because of its toxicity for some organisms. Cadmium (Cd) is a dangerous metal pollutant that can cause remarkable diverse of toxic effects, in particular for humans and animals. The use of bacteria as bioremediation agents has been widely studied because more efficient, less cost, and environmentally friendly strategy. This present study aimed to isolate and identify Cd-resistant bacteria from the industrial disposal site. Wastewater samples were collected from disposal site of agar flour industry in Lawang Malang, East Java. The collected wastewater effluent was analyzed for physicochemical properties. Isolation of Cd-resistant bacteria was carried out using serial dilution. Bacterial isolates were observed and tested for their effects on the content of Cd. The content of Cd was tested daily using Atomic Absorption Spectroscopy (AAS) for seven consecutive days. Data was analyzed using one-way ANOVA (p < 0.05) and Tukey test. Characterization of potential bacterium was performed using bacterial identification kit. Four bacteria isolates have been successfully isolated from the wastewater sample. There was a statistically significant difference between groups as determined by one-way ANOVA (F = 1229.62, p = 0.00). A Tukey post hoc test revealed that all conditions are significantly different from each other. The content of Cd in wastewater sample was statistically significantly lower after taking the A isolate (3.39 mg/L, p = 0.00), B Isolate (1.47 mg/L, p = 0.00), C Isolate (1.15 mg/L, p = 0.00), and D isolate (1.95 mg/L, p = 0.00) compared to the control treatment (5.11 mg/L, p = 0.00). Two of the most potential isolates identified as Pseudomonas flourescens (C isolate) and Enterobacter agglomerans (B isolate).
Isolasi DNA Genom Bakteri Potensial Pengkelat Logam Berat Kadmium dari Limbah Cair Penepungan Agar Pambudiono, Agung; Suarsini, Endang; Amin, Mohamad
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2016: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (423.352 KB)

Abstract

Bakteri mampu mengembangkan berbagai mekanisme resistensi dari sifat toksik logam berat sehingga penggunaan bakteri sebagai agen bioremidiasi logam berat mulai banyak dipelajari hingga tingkat molekuler. Berbagai analisis biologi molekuler memerlukan hasil isolasi DNA dengan tingkat kemurnian dan kualitas yang baik. Proses ekstraksi untuk mendapatkan DNA berkualitas tinggi merupakan satu kaidah dasar yang harus dipenuhi dalam analisis molekuler. Tujuan dari penelitian ini adalah mengisolasi DNA genom dari dua bakteri potensial pengkelat logam berat Kadmium (Cd) yang berasal dari limbah cair pengolahan tepung agar di Malang Jawa Timur. Isolasi DNA genom bakteri dilakukan dengan Presto™ Mini gDNA Bacteria Kit. Isolat DNA genom bakteri yang diperoleh diuji secara kuantitatif dengan metode spektrofotometri dan secara kualitatif dengan elektroforesis gel agarose. Hasil isolasi DNA genom pada bakteri B diperoleh hasil isolat DNA dengan konsentrasi sebanyak 1873,1 ng/μl dengan tingkat kemurnian (A260/280) sebesar2.04, sedangkan pada bakteri C diperoleh konsentrasi sebanyak 511.6 ng/μl dengan tingkat kemurnian (A260/280) sebesar 2,14. Hasil uji secara kualitatif menunjukkan bahwa panjang DNA genom yang berhasil diisolasi memiliki ukuran lebih dari 10000 bp baik pada sampel bakteri B maupun bakteri C.