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Dyah Ajeng Suhita
Universitas Airlangga
Agriculture, Biological Sciences & Forestry Immunology & microbiology Veterinary

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The Anthelmintic Potential of Ethyl Acetate Fraction Berenuk (Crescentia cujete L.) Fruit Against Mortality Haemonchus contortus In Vitro Dyah Ajeng Suhita; Anwar Ma'ruf; Agus Sunarso; Eduardus Bimo Aksono; Dewa Ketut Meles
Journal of Parasite Science (JoPS) Vol. 7 No. 1 (2023): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v7i1.37770

Abstract

This study aims to determine the anthelmintic potency of the ethyl acetate fraction of berenuk fruit (Crescentia cujete L.) on the mortality of the Haemonchus contortus worms in vitro. The method that was used in the research was a post-test only control group design. There were five treatments and each treatment was carried out in four repetitions. The sample used was twenty H. contortus in each treatment for all replications. Observation and recording of H. contortus mortality was carried out at 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60 minutes, and when all worms in the petri dish died. Data analysis using ANOVA, followed by Duncan’s Test and Probit analysis. The results showed that the ethyl acetate fraction of berenuk fruit had anthelmintic activity. The conclusion in this study is that the optimal concentration is found in the 0.5% ethyl acetate fraction concentration, the LC50 at each observation time successively is 1.39%; 0.97%; 0.70%; 0.48%; 0.39%; 0.34%; 0.31%; 0.28%; 0.27%; 0.26%; 0.25%; and 0.24%, while LT50 at a concentration of 0.125%; 0.25%; and 0.5% respectively are 1 hour 17 seconds, 41 minutes 42 seconds, and 20 minutes 58 seconds.
Correlation of Concentration Ethyl Acetate Fraction of Calabash Fruit (Crescentia cujete L.) with Mortality Haemonchus contortus Worm In Vitro Dyah Ajeng Suhita; Anwar Ma'ruf; Agus Sunarso; Eduardus Bimo Aksono Herupradoto; Dewa Ketut Meles
Journal of Parasite Science (JoPS) Vol. 6 No. 2 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v6i2.38973

Abstract

The purpose of this study was to ascertain how the concentration of the ethyl acetate component of calabash fruit (Crescentia cujete L.) affected the in vitro mortality of the Haemonchus contortus worm. This study employed a post-test only control group design as its methodology. There were five treatments, with a total of four repetitions. Twenty H. contortus worms were utilized as samples in each treatment throughout all replications. Observation and recording of H. contortus mortality was carried out at 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60 minutes, and when all worms in the petri dish died. Worm mortality was observed by examining whether there was a movement reaction from the worms when given a touch or water droplets at 50°C. Linear regression test was used to assess the data. The resulting linear equation, y = 2.417 + 5.875x, demonstrated that the ethyl acetate component of calabash fruit had an impact on worm mortality. The conclusion of this study is that the increase in the concentration of the ethyl acetate fraction is directly proportional to the mortality of the worm Haemonchus contortus in vitro with an effect value of 93.4%.
Co-Authors Aksono HP., Eduardus Bimo Anwar Ma'ruf Anwar Ma'ruf Meles, Dewa Ketut Sunarso, Agus