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All Journal Jurnal Sain Veteriner Microbiology Indonesia Indonesian Journal of Tropical and Infectious Disease Masyarakat, Kebudayaan dan Politik Jurnal Medik Veteriner Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology journal of Basic Medical Veterinary Journal of Pharmaceutical and Sciences.

Eduardus Bimo Aksono Herupradoto

Departemen Ilmu Kedokteran Dasar Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga

Author-ID : 1062306

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary

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QUICK DIAGNOSIS OF JAPANESE ENCEPHALITIS FOR NEW DIAGNOSED EMERGING DISEASE USING PCR TECHNIQUE IN SURABAYA, INDONESIA Matondang, Muhammad Qushai Y.; Nasronudin, Nasronudin; Aksono, Eduardus Bimo; Lusida, Maria Inge; Nastri, Aldise Mareta; Fajar, Nur Syamsiatul; Jannah, Lilis Mundri
Indonesian Journal of Tropical and Infectious Disease Vol 4, No 4 (2013)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (402.4 KB)

Background: Japanese enchepalitis (JE) is a viral disease that considered as zoonotic disease, which transmitted through mosquito vectors that had JE virus. Mainly caused by the mosquito C. Tritaeniorhynchus (the most important vector is the mosquito Culex, which feeds on cattle in preference to human). JE virus disease can also cause disturbances in the central nervous system eg. brain, bone marrow, and meninges which has serious impact on public health. This disease has been reported from Japan, Korea, Taiwan, India, Myanmar, Thailand, Western Pacific and Southeast Asia to Indonesia. However, the incidence of this disease in Indonesia has not been well known in various animal species or humans. Aim: The purpose of this study is to develop rapid diagnostic examinations on patient diagnosed JE virus in Surabaya by using PCR (Polymerase Chain Reaction). Because, JE disease can lead to dead-end at the patient if not treated immediately. Method: The research methods, extraction method, PCR (1stÂ RT-PCR and 2ndÂ Nested PCR) are conducted using Japanese encephalitis PCR detection kit. Result: The results of the examination showed that 2 out of 17 people (11,765%) are positive with PCR bands 227 bp (basepair). This diagnostic technique to determine and to deal with early onset of the disease. Solutions for preventive actions can be started from the termination of the cycle vectors to vaccination measures. Conclusion: For his own medical factors given to reduce fever and swelling and reduce the pain.

Respon Imun Mencit terhadap Protein 24 dan 71 kDa Toxocara vitulorum dalam Membentuk Antibodi dan Protektifitasnya terhadap Infeksi Buatan Candra Dwi Atma; Kusnoto Kusnoto; Eduardus Bimo Aksono HP.
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This study aimed to get 24 and 71 kDa protein of T. vitulorum that have a high antigenicity and imunogenicity on ELISA and to get the protein which able to protect mice against artificial infection of L2 T. vitulorum. This study using mice Balb/c aged 6 to 8 weeks. Proteins isolated were 24 and 71 kDa. Proteins 24, 71 kDa and intestinal homogenates immunized in mice with the addition of adjuvant (1: 1) for 3 times with period of 2 weeks. Two weeks after the last booster, serum drawn from mice tested by Indirect ELISA to determine the value of optical density (OD). The next stage, mice were infected L2 with a dose of 10-17 larvae / g of body weight. The results showed the average OD value by ANOVA Factorial antigen P24 was not significantly different with antigen P71 T. vitulorum. Antigen 24 kDa and 71 kDa with different immunization, both were showed P0 significantly different with P1, P2 and P3. Based on percentage of L2 in the somatic tissue of mice, P0 were showed 79.1% of total number of L2 early infection, whereas the treatment of P1 were showed 0.04%, P2 and P3 showed as much as 0.02% and 0.04%. 24 and 71 kDa protein of T. vitulorum that have a high antigenicity and imunogenicity.

Analyses of Precore and Core Promoter Mutations of Hepatitis B Virus in Patients with Chronic Hepatitis B in Surabaya, Indonesia . JUNIASTUTI; EDUARDUS BIMO AKSONO; TAKAKO UTSUMI; YOSHIHIKO YANO; . SOETJIPTO; YOSHITAKE HAYASHI; HAK HOTTA; FEDIK ABDUL RANTAM; HERNOMO ONTOSENO KUSUMOBROTO; MARIA INGE LUSIDA
Microbiology Indonesia Vol. 4 No. 3 (2010): December 2010
Publisher : Indonesian Society for microbiology

Mutations of precore (A1896) and core promoter (T1762/A1764) of hepatitis B virus can reduce HBeAg production. These mutations are frequently found in the late HBeAg seroconversion. However, it has been a controversy about the role played by precore and core promoter mutations in determining outcome of chronic hepatitis B. In the present study, the variability of precore and core promoter of hepatitis B virus were analyzed using PCR amplification and sequencing, according to the outcome (viral load and HBeAg/anti-HBe) in chronic hepatitis B patients in Surabaya. The study groups included 5 patients with uncomplicated chronic hepatitis B and 10 patients with chronic hepatitis B and liver cirrhosis in Dr. Soetomo Hospital, Surabaya. The control group included 6 blood donors obtained from Indonesia Red Cross, Surabaya. All groups were HBsAg positive. Precore mutation A1896 was predominant in all groups (60%-67% of each), together with precore variant T1858. As reported, precore variant T1858 is a prerequisite for precore A1896 and characteristic for viral genotype. Nevertheless, core promoter mutations T1762/A1764 were predominant only in LC patients (60%). All of these mutations were found mostly after HBeAg seroconversion (anti-HBe+). Of most samples with anti-HBe+, precore mutation was related with low viral load (<105 copies/mL), but core promoter mutations with high viral load (>105 copies/mL). Precore mutation A1896 was predominant in all groups, but core promoter mutations T1762/A1764 were only predominant in LC patients. The precore mutation alone is possible not critical to indicate a poor outcome, the core promoter mutations must be considered also.

Family-based collaborative eco-literacy model for sustainable city Sudar Itafarida; Eduardus Bimo Aksono Herupradoto; Usma Nur Dian Rosyidah; Erlita Rusnaningtias
Masyarakat, Kebudayaan dan Politik Vol. 32 No. 2 (2019): Masyarakat, Kebudayaan dan Politik
Publisher : Faculty of Social and Political Science, Universitas Airlangga

The process of adaptation and adoption of modern values by multicultural urban communities raises various problems, especially those related to the environment. In Surabaya and Sidoarjo, even though the Surabaya City Government and the Regional Government of Sidoarjo have carried out various environmental programs, the demands of a fast and practical modern lifestyle create egocentric and anthropocentric attitudes. Urban communities must take on the continuous socialization of ecocentric behavior that adopts modern values but must also maintain the value of local wisdom to support the realization of sustainable city. The family-based collaborative eco-literacy model is needed as a strategy to develop green living cultural values that can be practiced by all levels of society in various regions with different characteristics in both cities. This model directly touches the family as the main place to cultivate ecocentric mindset and behavior. Another consideration is that there is few government policies related to environment that directly target the family. This study illustrates how the family-based collaborative ecoliteracy model was applied to families in Surabaya and Sidoarjo and explains how the model has moved the attitudes and behavior of these families to those in line with ecocentric principles. By using a qualitative-descriptive approach, this model was applied to 16 families selected according to participant criteria. The subsequent changes in behavior were observed over a period of six months. The results of the application of the model show that each individual in the family collaborates and strengthens due to collective competence, which is an ecocentric value. Based on the orientation of green living values in eleven sustainable urban components, it can be concluded that almost all green city programs carried out by the government are intended to realize Surabaya and Sidoarjo as sustainable cities.

Prevalence of Monogenean Helminth Ectoparasites on Catfish (Clarias gariepinus) Culture Ponds in Laban Village, Menganti District, Gresik Regency, East Java Province Sri Subekti Bendryman; Adelina Grace; Eduardus Bimo Aksono
Journal of Parasite Science (JoPS) Vol. 1 No. 1 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

The aims of this study are to know the prevalence of monogenean helminth ecto-parasites infection on catfish culture, to know determine the number of monogenean infection on 100 samples consists of 1 and 3 months old of catfish. This study was conducted on May to June, 2011 in catfish culture ponds, Laban Village Menganti District and Gresik Regency, East Java Province. The methods were used in this research were skin and gill scrapping. The data were analyzed by using chi square test with SPSS program version 18.0 for windows. The results showed that prevalence of monogenean in catfish culture ponds, Laban Village Menganti District Gresik Regency, East Java Province was 32%. The highest infection was in the young catfish compared with the older ones with percentage of 23% and 9% respectively and based on chi square test showed that there were a very significantly differences (p < 0.01).

The Influence of Oral Administration of Red Dragon Fruit Peels Extract (Hylocereus polyrhizus) on Increasing High Density Lipoprotein in Male White Rats (Rattus norvegicus) Hypercholesterolemia Ahmad Thoriqul Firdaus; Gandul Atik Yuliani; Tri Wahyu Suprayogi; Nusdianto Triakoso; Eduardus Bimo Aksono Herupradoto; Nanik Hidayatik
Journal of Applied Veterinary Science And Technology Vol. 4 No. 1 (2023): April 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V4.I1.2023.1-4

Background: Hypercholesterolemia is one of the most common diseases in the world, with cholesterol levels exceeding normal levels. Hypercholesterolemia can cause various systemic disorders in the body. One of the signs is by decreasing HDL (High-Density Lipoprotein) cholesterol levels. Dragon fruit peel contains many ingredients, such as vitamin B3 (niacin), vitamin C, flavonoids, anthocyanins, and other minerals that can affect the increase in HDL production and reduce total cholesterol levels. Purpose: To know the effect of red dragon fruit peel extract (Hylocereus polyrhizus)on increasing levels of High-Density Lipoprotein (HDL) in male rats (Rattus norvegicus) hypercholesterolemia. Methods: Male rats were made hypercholesterolemic using a mixture of lard (3 ml) and egg yolk (1 g) for 14 days orally. 20 hypercholesterolemic male rats were further divided into 5 treatment groups and given dragon fruit peel extract therapy with different treatments, namely T0 negative control (CMC-Na 1% + aquadest), T1 positive control (Simvastatin 0.0013 mg/200 g BW), T2, T3 and T4 (each 100, 150, 200 mg/kg BW red dragon fruit peel extract) for 14 days orally. Blood serum was taken on day 36 and tested in the laboratory using a chemical analyzer machine; then the data were analyzed using the univariant multiple way one-way ANOVA statistical test with a significant difference (p>0.05). Results: The P4 treatment group has an effective dose (200 mg/kg BW) to increase high-density lipoprotein (HDL) levels, while the P3 treatment group (dose 150 mg/kg BW) is less effective but can still have an effect. Conclusion: This research showed that red dragon fruit peel extract could increase HDL (High-Density Lipoprotein) cholesterol levels with an effective 200 mg/kg BW dose.

Red dragon (Hylocereus polyrhizus) fruit peel extract increased the motility and viability of spermatozoa of hypercholesterolemic rats (Rattus norvegicus) Niken Meyliana Sari; M. Gandul Atik Yuliani; Eduardus Bimo Aksono Herupradoto; Nanik Hidayatik; Nurhusien Yimer; Tatik Hernawati
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 1 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i1.2023.34-42

This study aims to determine the effect of red dragon (Hylocereus polyrhizus) fruit peel extract (RDFPE) on spermatozoa motility and viability of hypercholesterolemic rats (Rattus norvegicus) as a model. Twenty male rats were randomly divided into negative control (NC), positive control (PC), treatment 1 (T1), treatment 2 (T2), and treatment 3 (T3) groups. All rats were given 2 mL of high cholesterol feed orally every day for 28 days. On day-15, all rats were measured for their blood cholesterol levels, followed by treatment for 14 days. Rats in the NC, PC, T1, T2, and T3 groups were treated with 1% Na-CMC, Simvastatin 10 mg/kg BW, and RDFPE of 500, 750, and 1000 mg/kg BW, respectively. On day-29, all rats were sacrificed to evaluate spermatozoa viability and motility. The results showed that spermatozoa viability and motility in the hypercholesterolemic rats (NC) group were the lowest (p <0.05) among the groups. Treatment of hypercholesterolemic rats with Simvastatin 10 mg/kg BW (PC) group showed higher (p <0.05) spermatozoa viability and motility compared to the NC group. RDFPE dose of 1000 mg/kg BW (T3 group) resulted in higher (p <0.05) spermatozoa viability and motility compared to other RDFPE doses (T1 and T2) and the control (NC) groups, and it was similar (p >0.05) compared with the Simvastatin treated (PC) group. It could be concluded that the administration of 1000 mg/kg BW ethanolic extract of red dragon fruit (Hylocereus polyrhizus) peel increased the viability and motility of spermatozoa of hypercholesterolemic rats (Rattus norvegicus) which were the same as the group of rats given Simvastatin 10 mg/kg BW.

Correlation of Concentration Ethyl Acetate Fraction of Calabash Fruit (Crescentia cujete L.) with Mortality Haemonchus contortus Worm In Vitro Dyah Ajeng Suhita; Anwar Ma'ruf; Agus Sunarso; Eduardus Bimo Aksono Herupradoto; Dewa Ketut Meles
Journal of Parasite Science (JoPS) Vol. 6 No. 2 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v6i2.38973

The purpose of this study was to ascertain how the concentration of the ethyl acetate component of calabash fruit (Crescentia cujete L.) affected the in vitro mortality of the Haemonchus contortus worm. This study employed a post-test only control group design as its methodology. There were five treatments, with a total of four repetitions. Twenty H. contortus worms were utilized as samples in each treatment throughout all replications. Observation and recording of H. contortus mortality was carried out at 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60 minutes, and when all worms in the petri dish died. Worm mortality was observed by examining whether there was a movement reaction from the worms when given a touch or water droplets at 50Â°C. Linear regression test was used to assess the data. The resulting linear equation, y = 2.417 + 5.875x, demonstrated that the ethyl acetate component of calabash fruit had an impact on worm mortality. The conclusion of this study is that the increase in the concentration of the ethyl acetate fraction is directly proportional to the mortality of the worm Haemonchus contortus in vitro with an effect value of 93.4%.

QUICK DIAGNOSIS OF JAPANESE ENCEPHALITIS FOR NEW DIAGNOSED EMERGING DISEASE USING PCR TECHNIQUE IN SURABAYA, INDONESIA Matondang, Muhammad Qushai Y.; Nasronudin, Nasronudin; Aksono, Eduardus Bimo; Lusida, Maria Inge; Nastri, Aldise Mareta; Fajar, Nur Syamsiatul; Jannah, Lilis Mundri
Indonesian Journal of Tropical and Infectious Disease Vol. 4 No. 4 (2013)
Publisher : Institute of Topical Disease Universitas Airlangga

Background: Japanese enchepalitis (JE) is a viral disease that considered as zoonotic disease, which transmitted through mosquito vectors that had JE virus. Mainly caused by the mosquito C. Tritaeniorhynchus (the most important vector is the mosquito Culex, which feeds on cattle in preference to human). JE virus disease can also cause disturbances in the central nervous system eg. brain, bone marrow, and meninges which has serious impact on public health. This disease has been reported from Japan, Korea, Taiwan, India, Myanmar, Thailand, Western Pacific and Southeast Asia to Indonesia. However, the incidence of this disease in Indonesia has not been well known in various animal species or humans. Aim: The purpose of this study is to develop rapid diagnostic examinations on patient diagnosed JE virus in Surabaya by using PCR (Polymerase Chain Reaction). Because, JE disease can lead to dead-end at the patient if not treated immediately. Method: The research methods, extraction method, PCR (1st RT-PCR and 2nd Nested PCR) are conducted using Japanese encephalitis PCR detection kit. Result: The results of the examination showed that 2 out of 17 people (11,765%) are positive with PCR bands 227 bp (basepair). This diagnostic technique to determine and to deal with early onset of the disease. Solutions for preventive actions can be started from the termination of the cycle vectors to vaccination measures. Conclusion: For his own medical factors given to reduce fever and swelling and reduce the pain.

NO ASSOCIATION OF RECURRENT RESPIRATORY PAPILLOMATOSIS AGGRESSIVENESS AND HUMAN PAPILLOMAVIRUS TYPE 6 AND 11 Perdana, Rizka Fathoni; Herawati, Sri; Suroso, Bakti; Aksono, Eduardus Bimo
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 5 (2017)
Publisher : Institute of Topical Disease Universitas Airlangga

Recurrent Respiratory Papillomatosis (RRP) is the most common benign neoplasm of the larynx among children as a result of HPV infection mainly type 6 and 11. RRP is still considered as serious problem since papilloma in the airway can cause hoarseness and obstruction which later described as aggressive and non aggressive types. Patients underwent multiple surgeries to keep airway patency and in order to communicate vocally. Previous studies reported that HPV-11 was associated with a more aggressive disease course. This study aim is to determine the association of RRP aggressiveness with HPV-6 and 11. Papilloma specimens were taken from patients who underwent surgical treatment of RRP patients and subjected HPV typing. All 17 patients with completed data and epidemiologic questionaires were defined for their staging to disease severity. Informations in the database were analyzed to identity statistically significant relationship with HPV type and knowing the HPV type is to predict the disease course. 17 patients RRP (12 males and 5 females) with age at onset 3,15 + 2,28 years and age at diagnosis 3,81 + 2,51 years. Surgery was performed 2,71 ± 1,16 times per year and 9 patients treated more than 3 surgeries per year. 10 patients had distal papilloma and 11 patients had tracheotomy. Agrressive disease was show found in 14 patients among 17 patients. HPV-11 was detected in 10 patients, HPV-6 was detected in 7 patients. According to the statisitcal analysis (Chi square test), there is no relationship between HPV type and disease aggressiveness. HPV-6 and 11 are not the only cause that affect the aggressiveness of Recurrent Respiratory Papillomatosis.

Co-Authors Adelina Grace Ahmad Thoriqul Firdaus Al-Arief, Mohamad Anam Aldise Mareta Nastri, Aldise Mareta Anwar Ma'ruf Billa, Lutfiah Annisa Boedi Setiawan Candra Dwi Atma Dyah Ajeng Suhita Endang Suprihati Erlita Rusnaningtias Fedik Abdul Rantam Gandul Atik Yuliani HAK HOTTA Heni Puspitasari HERNOMO ONTOSENO KUSUMOBROTO Hidayatik, Nanik Ira Sari Yudaniayanti Juniastuti Juniastuti Kadek Rachmawati Kusnoto Kusnoto Leonardo Reza Srimaryanto Lilis Mundri Jannah, Lilis Mundri Lucia Tri Suwanti, Lucia Tri Maria Inge Lusida MARIA INGE LUSIDA Meles, Dewa Ketut Mirni Lamid Mochamad Lazuardi Mufasirin Muhammad Qushai Y. Matondang, Muhammad Qushai Y. Nasronudin Nasronudin Niken Meyliana Sari Nove Hidajati Nur Syamsiatul Fajar, Nur Syamsiatul Nurfitri, Yulia Nurhusien Yimer Nusdianto Triakoso Nusdianto Triakoso Perdana, Rizka Fathoni Puput, Sesa SOETJIPTO . Sri Herawati Sri Subekti Sudar Itafarida Sunarso, Agus Suroso, Bakti TAKAKO UTSUMI Tatik Hernawati Tri Wahyu Suprayogi Usma Nur Dian Rosyidah Wahyuni, Retno Sri Wiwik Misaco Yuniarti YOSHIHIKO YANO YOSHITAKE HAYASHI Yuliani, Margareth Gandul Atik Yunus, Muchammad

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