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All Journal BIOTROPIC
Arif Rahman Hikam
Program Studi Biologi, Fakultas Biologi, Universitas Jenderal Soedirman, Purwokerto, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Karakteristik Bakteri Pendegradasi Bahan Pangan Asal Saluran Pencernaan Kecoa Amerika (Periplaneta americana) dari Pasar Tradisional Dwiana Muflihah Yulianti; Arif Rahman Hikam; Trisnowati Budi Ambarningrum; Taruna Dwi Satwika; Dyah Fitri Kusharyati; Hilmiyah Al'alawiyah
Biotropic : The Journal of Tropical Biology Vol. 7 No. 1 (2023): Biotropic, Volume 7 Nomor 1, 2023
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v7i1.1662

Abstract

American cockroach (Periplaneta americana) is one of the most common pests found in houses, apartments, and public facilities such as markets. As vector insects, cockroaches have symbiosis with microorganisms. Some of these microorganisms can live in cockroaches' cuticles and digestive tracts. Besides being able to pose a danger to human health, the microbes in cockroach bodies also have the potential to cause food damage. This study aimed to determine the diversity of microorganisms originating from the digestive tract of the American cockroach and their potential to cause food spoilage. The research was conducted in several stages: sampling cockroaches at a traditional market, isolating bacteria from the digestive tract of cockroaches, qualitatively testing amylolytic, proteolytic, and lipolytic potentials, and characterizing bacterial isolate by morphologically and biochemically character. The results showed that there were 26 isolates of bacteria isolated from the digestive tract of American cockroaches from the market, 62.96% of the isolates were found to have the ability to degrade protein, 37.04% of the isolates had the ability to degrade starch, and 40.74% of the total isolates can degrade fat. These isolates had various morphological and biochemical characteristics. Bacterial isolates derived from American cockroaches from traditional markets have the potential to degrade food.
Potensi Fungi Rizosfer Tanaman Damar (Agathis dammara) Sebagai Agensia Biokontrol Fusarium oxysporum Galang Anahatta Hidayat; Arif Rahman Hikam; Yuriza Eshananda; Brenda Liestyaning Adi; Tyas Wanda Septiani
Biotropic : The Journal of Tropical Biology Vol. 8 No. 1 (2024): Biotropic, Volume 8 Nomor 1, 2024
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v8i1.2066

Abstract

Plant diseases become one of the dangerous threats to agriculture. Fusarium oxysporum is one of pathogen that attack many agricultural plants. The spread of plant pathogens must be controlled. One of the way to control plant pathogens is to use biological control methods by utilizing biocontrol agents using antagonistic fungi to control F. oxysporum. Antagonistic fungi can be found in various habitats such as rhizosphere soil that contains a lot of exudate released by the roots so that the area is rich in organic compounds. Sampling was carried out in the rhizosphere soil of Damar plants from the Baturraden Botanical Garden. Research on antagonistic fungi from the plant rhizosphere needs further exploration. Therefore, this study was conducted to obtain isolates of rhizosphere fungi that have potential as antagonists against F. oxysporum. This research was carried out with method stages ranging from sampling, isolation, characterization and identification to antagonism tests. The isolation results stated that five different fungi were found from the rhizosphere of the Damar plant. The fungi found were then tested for antagonistic ability against F. oxysporum and it was found that R5 isolate had the best ability to inhibit the growth of F. oxysporum with an inhibitory percentage value of 72.06%. This ability can be new information to be able to develop further about the potential of large plant rhizosphere fungi in their inhibition against plant pathogens.
Co-Authors Brenda Liestyaning Adi Dwiana Muflihah Yulianti Dyah Fitri Kusharyati Galang Anahatta Hidayat Hilmiyah Al'alawiyah Taruna Dwi Satwika Trisnowati Budi Ambarningrum Tyas Wanda Septiani Yuriza Eshananda