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Yuriza Eshananda
Program Studi Biologi, Fakultas Biologi, Universitas Jenderal Soedirman, Purwokerto, Indonesia

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Keragaman Morfologi Bakteri Nitrifikasi Asal Kompos Kotoran Domba pada Peternakan Domba dengan Sistem Bedding Sri Martina Wiraswati; Hendro Pramono; Oedjijono; Dini Ryandini; Dyah Fitri Kusharyati; Meyta Pratiwi; Taruna Dwi Satwika; Dwiana Muflihah Yulianti; Saefuddin Aziz; Daniel Joko Wahyono; Rizal Khoirun Alfisah; Yuriza Eshananda; Budi Rustomo; Afifah Mariana
Biotropic : The Journal of Tropical Biology Vol. 7 No. 1 (2023): Biotropic, Volume 7 Nomor 1, 2023
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v7i1.1695

Abstract

The application of compost bedding system on sheep farm is a technology to minimize the negative impact of sheep manure waste toward environment. The microorganisms which inhabit the compost bedding, especially nitrifying bacteria are expected to have significant role in odor emission reduction which become environmental problem around the sheep farm. This study aims to analyze the abundance and diversity of nitrifying bacteria of compost bedding obtained from sheep farm which have important role in reducing odor emission of ammonia. The 12 days, 1 and 1,5 months old of compost bedding samples were obtained from sheepfold, while 3 and 4 months old of compost samples were obtained from compost pile. Furthermore, nitrifying bacteria were isolated from compost samples by pour plate method using specific media for Nitrosomonas and Nitrobacter. The results indicated that compost bedding samples with different composting time duration displayed different abundance and diversity of nitrifying bacteria. The current study was successfully isolated 39 and 47 nitrifying bacterial isolates using specific media for Nitrobacter and Nitrosomonas, respectively. The highest diversity of nitrifying bacteria was gained from 12 days old compost bedding sample. Nitrifying bacterial isolates from compost bedding samples have significant role in odor emission declining as well as manure composting at sheep farm. The obtained bacterial isolates are also potentially to develop as bio-activator for compost bedding.
Potensi Fungi Rizosfer Tanaman Damar (Agathis dammara) Sebagai Agensia Biokontrol Fusarium oxysporum Galang Anahatta Hidayat; Arif Rahman Hikam; Yuriza Eshananda; Brenda Liestyaning Adi; Tyas Wanda Septiani
Biotropic : The Journal of Tropical Biology Vol. 8 No. 1 (2024): Biotropic, Volume 8 Nomor 1, 2024
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v8i1.2066

Abstract

Plant diseases become one of the dangerous threats to agriculture. Fusarium oxysporum is one of pathogen that attack many agricultural plants. The spread of plant pathogens must be controlled. One of the way to control plant pathogens is to use biological control methods by utilizing biocontrol agents using antagonistic fungi to control F. oxysporum. Antagonistic fungi can be found in various habitats such as rhizosphere soil that contains a lot of exudate released by the roots so that the area is rich in organic compounds. Sampling was carried out in the rhizosphere soil of Damar plants from the Baturraden Botanical Garden. Research on antagonistic fungi from the plant rhizosphere needs further exploration. Therefore, this study was conducted to obtain isolates of rhizosphere fungi that have potential as antagonists against F. oxysporum. This research was carried out with method stages ranging from sampling, isolation, characterization and identification to antagonism tests. The isolation results stated that five different fungi were found from the rhizosphere of the Damar plant. The fungi found were then tested for antagonistic ability against F. oxysporum and it was found that R5 isolate had the best ability to inhibit the growth of F. oxysporum with an inhibitory percentage value of 72.06%. This ability can be new information to be able to develop further about the potential of large plant rhizosphere fungi in their inhibition against plant pathogens.