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All Journal JURNAL BIOLOGI PAPUA
Verena Agustini
Cenderawasih University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Materials Science & Nanotechnology

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Phytochemical Screening, Antibacterial and Cytotoxic Activity of Dendobium lasianthera J.J.Sm. from Papua Verena Agustini; Eva S. Simaremare; Elsye Gunawan; Jane Awom; Susan Wopi
Jurnal Biologi Papua Vol 12, No 2 (2020)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.079 KB) | DOI: 10.31957/jbp.1289

Abstract

The aims of the study are to evaluate bioactive compounds, antibacterial and cytotoxic potential of D. lasianthera. This orchid grows well all over New Guinea Island as an ornamental plant because of their beautiful flowers. Orchids also known rich of its phytochemical compounds which already used as a traditional medicine in many countries around the world. However, research in pharmacological fields is still limited. In this study, leaves and stem of D. lasianthera were powdered and extracted with ethanol followed by fractionated using n-hexane, ethyl acetate, and ethanol solvent. Extract as well as fraction were tested for phytochemical screening and determined antibacterial activity against Escherichia coli and Staphylococcus aureus using Disc Diffusion Method. Brine Shrimp Lethality Test (BSLT) was used to observe cytotoxic potential of leaves and stem extract and fraction at 10, 50, 100, 250, 500, 750, and 1000 ppm. The results showed that the leaves and stem extract contained tannin and alkaloids, separately. The ethanol extract of D. lasianthera showed 7.35 mm (leaves) and 7.43 mm (stem) inhibition zone against S. aureus Furthermore, the maximum inhibition zone of ethanol fraction of leaves and n-hexane fraction of stem were 8.42 mm and 8.10 mm separately. The LC50 of stem extract and fractions in these study were 699.3 ppm (ethanol extract), 602.1 ppm (ethyl acetate), 329.6 ppm (n-hexane fraction) and 676 ppm (ethanol fraction), whereas for leaves, only ethyl acetate fraction has toxict activity with an average LC50 833.2 ppm. Key words: D. lasianthera; phytochemical screening; antibacterial; cytotoxicity; Papua. 
Studi Awal Regenerasi Sowang (Xanthostemon novaguineense Valet.) Sri Wilujeng; Verena Agustini
Jurnal Biologi Papua Vol 9, No 1 (2017)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (345.816 KB) | DOI: 10.31957/jbp.106

Abstract

Sowang (Xanthostemon novaguineense Valet.) is an endemic plant of Cycloops and strictly distributed. Since years, the population of the plants is threatened by human activities through land conversion, forest burning and logging. There are only few seedling grow in Cycloops naturally. Sowang wood is known as a good quality, because of its inherent hardness and high density. Scienfific information about this species is remain unclear. Regarding the plant regeneration, it is also not enough information so far. The study was conducted at three places around Cycloops areas namely Waena, Doyo Baru (sowing habitat) dan Wambena (no sowang areas) of Jayapura Regency, Papua and also greenhouse of FMIPA Cenderawasih University. The experimental units were set up in a completely randomized design with 3 treatments. Each experiment was carried out in three replicated and each replicate includes 100 seeds. Data were analyzed by analysis of variance (ANOVA), followed by LSD α=0.05. The result showed that there is no relation between geographical distribution of Sowang at Cycloops and the phisical and chemical soil. All type of soil used in this study was good as media of the seed germination. Regarding the ratio of the sapling population, the germination is still low only 18%, the seedling only survive for 38 days after sowing. Key words: Cycloops, regeneration, Sowang, Papua.
Co-Authors Elsye Gunawan Jane Awom Sri Wilujeng Susan Wopi