Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal BERITA BIOLOGI
Rizkita RE, Rizkita
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
PENGARUH PEMBERIAN EKSTRAK Saccharomyces cerevisiae Hansen TERHADAP KANDUNGAN GOSIPOL PADA KULTUR KALUS Gossypium hirsutum L. Sitinjak, Rama Riana; Siregar, Arbayah H; RE, Rizkita
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.19 KB) | DOI: 10.14203/beritabiologi.v5i2.1133

Abstract

This study concentrated on the effect of Saccharomyces cerevisiae Hansen extract on gosipol content of Gossypium hirsutum L callus cultures. There were many ways commonly used to obtain high secondary metabolites product by using tissue culture,i.e. elicitation.The objective of this study was to increase the production ofgossypol on G. hirsutum callus cultures using elicitor derived from S. cerevisiae.The callus grew optimally and produced gossypol on Linsmaierand Skoog (LS) medium with the addition of 10 M NAA and 10 M 2,4-D. Callus derived from cotyledon was subcultured five times.Callus was then elicited with elicitor derived from autoclaved S. cerevisiae and concentration of elicitor tested were 0.5, 1.0 and 2.5% (b/v). The harvesting times were 0, 2, 4, dan 6 days after elicitation. The gossypol was analyzed qualitatively and quantitatively by using High Performance Liquid Chromatography (HPLC).Gossypol content was influenced significantly by concentration of elicitor and harvesting time.The maximum ofgossypol content was obtained on the 4 day after elicitation i.e. 469.233 ± 2.332 pg/g dry weight in the elicited callus of G. hirsutum.
PENGARUH PEMBERIAN ELISITOR EKSTRAK KHAMIR Saccharomyces cerevisiae Hansen TERHADAP KANDUNGAN AJMALISIN DALAM KULTUR AGREGAT SEL Catharanthus roseus (L.) G. Don. Ratnasari, Jujun; Siregar, Arbayah H; RE, Rizkita
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (389.353 KB) | DOI: 10.14203/beritabiologi.v5i4.1118

Abstract

There were many ways to obtain high production of secondary metabolites in plant tissue culture; among the other is elicitation.An experiment to study the effect of elicitor derived from Saccharomyces cerevisiae Hansen extract on ajmalicine content in cell aggregates culture of Catharanthus roseus (L.) G.Don, has been conducted. The media used for callus induction and cell aggregates culture were Zenk 6 5(1977) with addition of 2.5 x 10" M Naphthalene Acetic Acid (NAA) and 10 M 6-Benzilaminopurine (BAP).The cell aggregates culturewas subcultured three times and then elicitated with elicitor derived from autoclaved 5.cerevisiae extract at concentrations 0.5, 1.0, 2.5%,and harvested at 18, 24,and 36 hours after elicitation.The ajmalicine was analyzed qualitatively and quantitatively by using High Pressure Liquid Chromatography (HPLC) connected to chromatopack CR-7A Plus. The cell aggregates of C. roseus culture produced ajmalicine both in the cells and the media.The result of elicitation showed that ajmalicine content was influenced significantly by concentration and harvesting time. The highest ajmalicine content in the cell aggregates was 25.288 ± 0.102 jig/g dw, whilst that in media was 524.600 ± 0.566 \ML. The optimum concentration of S. cerevisiae extract was 0.5%, and the best harvesting time was 24 hours.
Co-Authors Arbayah H Siregar, Arbayah H Jujun Ratnasari Rama Riana Sitinjak