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THE EFFECT OF PLANT GROWTH REGULATOR TRIACONTANOL TO THE GROWTH OF CACAO SEEDLINGS (Theobroma cacao L.) Sitinjak, Rama Riana; Pandiangan, Dingse
AGRIVITA, Journal of Agricultural Science Vol 36, No 3 (2014)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

The aim of this study was to evaluate the effect of  plant growth regulator triacontanol to the growth of cacao seedings (Theobroma cacao L.). A completely randomized design was applied using non-factorial pattern on the treatment triacontanol growing regulator substance to level: 0.0 ml/L, 0.1 ml/L, 0.5 ml/L, 1.0 ml/L and 2.0 ml/L replicated 5 times. The result shows that the given of tria-contanol significantly affected the growth of cacao seedings at the age of 14 weeks. The best tria-contanol concentration was 1.0 ml/L, which was effectively able to increase the growth of cacao seedlings; high seedlings, number of leaves, lenght of leaves, and diameter of stems respectively reached 26.21%; 3.70%; 42.28%; and 10.42% higher than the control. Plant growth regulator triacontanol was efective to increase the growth of cacao seedlings. Keywords: triacontanol, seedling, Theobroma cacao L.
PENGARUH 2,4-D DAN BA TERHADAPINDUKSIKALUS EMBRIOGENIK PADA KULTUR MERISTEM JAHE {Zingiber officinale Rose.) Sitinjak, Rama Riana; Rostiana, Otih; Karyono, Karyono; Supriatun, Titin
BERITA BIOLOGI Vol 8, No 2 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i2.2037

Abstract

The aim of this study was to evaluate the effects of 2,4-dichlorophenoxyacetic acid (2,4-D) and N -benzyladenin (BA) to embryogenic callus induction of meristem culture in ginger {Zingiber officinale Rose). Completely Randomixed Design was applied using factorial pattern, replicated 3 times. Two factors were assigned i.e: 2,4-D (1.0, 2.0 and 3.0 mg/L) and BA (0.0, 1.0, 3.0 and 5.0 mg/L). Result showed that 2,4-D and BA significantly affected the embryogenic callus induction. The highest embryogenic callus (93.33%) was achieved on MS (Murashige & Skoog) containing combination of 1.0 mg/L 2,4-D and 3.0 mg/L BA, after 8weeks incubation.Histological evaluation informed that the incubation of embryogenic calli started from cortex cells adjacent the meristem vascular tissues.
PENGARUH PEMBERIAN EKSTRAK Saccharomyces cerevisiae Hansen TERHADAP KANDUNGAN GOSIPOL PADA KULTUR KALUS Gossypium hirsutum L. Sitinjak, Rama Riana; Siregar, Arbayah H; RE, Rizkita
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.19 KB) | DOI: 10.14203/beritabiologi.v5i2.1133

Abstract

This study concentrated on the effect of Saccharomyces cerevisiae Hansen extract on gosipol content of Gossypium hirsutum L callus cultures. There were many ways commonly used to obtain high secondary metabolites product by using tissue culture,i.e. elicitation.The objective of this study was to increase the production ofgossypol on G. hirsutum callus cultures using elicitor derived from S. cerevisiae.The callus grew optimally and produced gossypol on Linsmaierand Skoog (LS) medium with the addition of 10 M NAA and 10 M 2,4-D. Callus derived from cotyledon was subcultured five times.Callus was then elicited with elicitor derived from autoclaved S. cerevisiae and concentration of elicitor tested were 0.5, 1.0 and 2.5% (b/v). The harvesting times were 0, 2, 4, dan 6 days after elicitation. The gossypol was analyzed qualitatively and quantitatively by using High Performance Liquid Chromatography (HPLC).Gossypol content was influenced significantly by concentration of elicitor and harvesting time.The maximum ofgossypol content was obtained on the 4 day after elicitation i.e. 469.233 ± 2.332 pg/g dry weight in the elicited callus of G. hirsutum.
KEANEKARAGAMAN JAMUR PADA POHON KELAPA SAWIT DI PESISIR PANTAI PT. MOPOLI RAYA UNIT DAMAR CONDONG KAB. LANGKAT SUMATERA UTARA Rama Riana Sitinjak; Harmileni; Supriyadi
JURNAL PELITA KOTA Vol 2 No 2 (2021): JURNAL PELITA KOTA, AUGUST 2021
Publisher : Karimun University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51742/pelita.v2i2.420

Abstract

Pentingnya dalam keanekaragaman tersebut termasuk berbagai jenis jamur yang tumbuh di Indonesia dan telah lama dikenal serta dimanfaatkan oleh masyarakat sumber pangan dan sebagai bahan obat. Jamur atau cendawan ini turut memberikan andil besar dalam memenuhi aneka ragam atau menu makanan khas Indonesia, seperti tape, tempe, oncom, tauco, roti, minuman fermentasi, serta berbagai macam makanan lainnya. Jamur juga berperan penting sebagai decomposer yang dapat membentuk jaring-jaring makanan bagi tumbuhan atau ekosistem hutan. Penelitian ini bertujuan untuk mengetahui tingkat keanekaragaman jamur pada pohon kelapa sawit dengan jenis-jenis. jamur di perkebunan pesisir pantai. Metode yang digunakan adalah metode purposive sampling, yaitu teknik pengambilan sampel dengan menentukan kriteria-kriteria tertentu. Parameter penelitian terdiri frekuensi mutlak (FM), frekuensi relatif (FR), kerapatan mutlak (KM), kerapatan relatif (KR), dominasi, indeks nilai penting, perbandingan nilai penting (H). Data dianalisis dengan indeks keanekaragaman Shannon-Wiener. Hasil penelitian menunjukkan bahwa tingkat keanekaragaman jamur pada pohon kelapa sawit di perkebunan pesisir pantai PT. Mopoli Raya Unit Damar Condong Kab. Langkat Sumatera Utara masih tergolong rendah dengan Nilai Indeks H’ 0.33.
Analisis Vegetasi Gulma pada Perkebunan Kelapa Sawit (Elaeis quineensis Jacq.) di Desa Salang Tungir, Namorambe, Deli Serdang Siregar, Dendi Alfiansyah; Sitinjak, Rama R; Afrianti, Suratni; Agustina, Nur Ariyanti
JURNAL BIOS LOGOS Vol 11, No 2 (2021): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v11i2.34674

Abstract

(Article History: Received July 7, 2021; Revised August 25, 2021; Accepted August 28, 2021) ABSTRAKPenelitian ini bertujuan untuk mengetahui komposisi dan struktur gulma pada perkebunan kelapa sawit (Elaeis quineensis Jacq.) di Desa Salang Tungir Kecamatan Namorambe Kabupaten Deli Serdang.Penelitian ini dilaksanakan pada bulan Februari-Juni 2021. Metode yang digunakan adalah survey dengan teknik pencuplikan sampel secara purposive sampling dengan dua kali ulangan. Hasil penelitian ditemukan 8 famili, 10 spesies gulma dengan jumlah keseluruhan individu 934 gulma.Jenis gulma yang ditemukan terdiri dari kelompok gulma rumput, gulma berdaun lebar, berdaun sempit, dan pakis-pakisan.Nilai kerapatan gulma yang tertinggi adalah 469/m2 dengan nilai relatifnya 50,21% ditemukan pada  spesies gulma Dryopteris aridus, sedangkan yang terendah ditemukan pada spesies Emelia sonchifoliasebesar 10/m2 dengan nilai relatifnya 1,07%.Nilai frekuensi gulma yang tertinggi adalah 1,94 dengan nilai relatifnya 28,17% ditemukan pada spesies gulma Dryopteris aridus. Indeks nilai penting gulma yang tertinggi dperoleh pada lahan perkebunan kelapa sawit adalah sebesar 78,39% ditemukan pada jenis gulma Dryopteris aridus.Kata Kunci: gulma; Elaeis quineensis Jacq.; analisis vegetasi; kerapatan spesies ABSTRACTThis study aims to determine the composition and structure of weeds in oil palm plantations (Elaeis quineensis Jacq.) in Salang Tungir Village, Namorambe District, Deli Serdang Regency. This research was conducted from February to June 2021. The method used is a survey with purposive sampling technique on the plots with two replications. The results of the study found 8 families, 10 species of weeds with a total of 934 individuals. The types of weeds found consisted of grass weeds, broad leaf weeds, narrow leaf weeds, and ferns.The highest weed density value was 469/m2 with a relative value of 50.21% found in the weed species Dryopteris aridus, while the lowest was found in the Emelia sonchifolia species of 10/m2 with a relative value of 1.07%. The highest weed frequency value was 1.94 with a relative value of 28.17% found in the weed species Dryopteris aridus. The highest important value index of weeds was obtained on oil palm plantations, which was 78.39% and was found in Dryopteris aridus weed. Keywords: Weeds; Elaeis quineensis Jacq.; vegetation analysis; species density
Respon Pertumbuhan Bibit Kelapa Sawit di Tahap Pre-Nursery dengan Aplikasi Pupuk Organik Cair Kulit Pisang Kepok. Tengku Muhammad Sahiril Anhar; Rama R. Sitinjak; Edy Fachrial; Bayu Pratomo
AGRIUM: Jurnal Ilmu Pertanian Vol 24, No 1 (2021)
Publisher : UNIVERSITAS MUHAMMADIYAH SUMATERA UTARA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30596/agrium.v23i2.6915

Abstract

Kelapa sawit merupakan satu dari sekian banyak komoditas penyumbang terbesar bagi devisa negara. Pertumbuhan bibit kelapa sawit sangat ditentukan oleh ketersediaan unsur hara dengan pemanfaatan limbah organik seperti kulit pisang kepok dan urine domba. Hal tersebut akan mengurangi penggunaan pupuk kimia (anorganik) yang dapat menciptakan pertanian ramah lingkungan. Selain itu, berdasarkan refrensi yang penulis baca masih sangat jarang dijumpai penelitian mengenai pemanfaatan kulit pisang kepok menjadi pupuk organik cair terutama aplikasi pada pembibitan kelapa sawit. Penelitian ini memiliki tujuan untuk mengetahui respon pertumbuhan bibit kelapa sawit di tahap Pre-Nursery setelah diberikan kulit pisang kepok sebagai Pupuk Organik Cair (POC) dengan dosis yang berbeda. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) 1 faktorial. Faktornya adalah POC kulit pisang kepok yang terdiri dengan 4 taraf yaitu: Kontrol (A0), 50 ml (A1), 150 ml (A2), 300 ml (A3). Data dianalisis dengan menggunakan sidik ragam (analysis of variance) di lanjutkan Duncans Multiple Range Test dengan signifikan 5 %. Parameter yang diamati yaitu tinggi tanaman, diameter batang, jumlah daun, berat segar tajuk, berat kering tajuk, berat segar akar dan berat kering akar tanaman. Berdasarkan hasil dari penelitian ini bahwa pengaplikasian pupuk organik cair kulit pisang kepok tidak berpengaruh nyata terhadap respon pertumbuhan bibit kelapa sawit di tahap pre-nursery, namun bila berdasarkan data rataan, pengaplikasian 50 ml pupuk organik cair kulit pisang kepok mampu mendorong pertumbuhan bibit kelapa sawit pada tahap pre-nursery.
PENGARUH 2,4-D DAN BA TERHADAPINDUKSIKALUS EMBRIOGENIK PADA KULTUR MERISTEM JAHE {Zingiber officinale Rose.) Rama Riana Sitinjak; Otih Rostiana; Karyono Karyono; Titin Supriatun
BERITA BIOLOGI Vol 8, No 2 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i2.2037

Abstract

The aim of this study was to evaluate the effects of 2,4-dichlorophenoxyacetic acid (2,4-D) and N -benzyladenin (BA) to embryogenic callus induction of meristem culture in ginger {Zingiber officinale Rose). Completely Randomixed Design was applied using factorial pattern, replicated 3 times. Two factors were assigned i.e: 2,4-D (1.0, 2.0 and 3.0 mg/L) and BA (0.0, 1.0, 3.0 and 5.0 mg/L). Result showed that 2,4-D and BA significantly affected the embryogenic callus induction. The highest embryogenic callus (93.33%) was achieved on MS (Murashige & Skoog) containing combination of 1.0 mg/L 2,4-D and 3.0 mg/L BA, after 8weeks incubation.Histological evaluation informed that the incubation of embryogenic calli started from cortex cells adjacent the meristem vascular tissues.
PENGARUH PEMBERIAN EKSTRAK Saccharomyces cerevisiae Hansen TERHADAP KANDUNGAN GOSIPOL PADA KULTUR KALUS Gossypium hirsutum L. Rama Riana Sitinjak; Arbayah H Siregar; Rizkita RE
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i2.1133

Abstract

This study concentrated on the effect of Saccharomyces cerevisiae Hansen extract on gosipol content of Gossypium hirsutum L callus cultures. There were many ways commonly used to obtain high secondary metabolites product by using tissue culture,i.e. elicitation.The objective of this study was to increase the production ofgossypol on G. hirsutum callus cultures using elicitor derived from S. cerevisiae.The callus grew optimally and produced gossypol on Linsmaierand Skoog (LS) medium with the addition of 10 M NAA and 10 M 2,4-D. Callus derived from cotyledon was subcultured five times.Callus was then elicited with elicitor derived from autoclaved S. cerevisiae and concentration of elicitor tested were 0.5, 1.0 and 2.5% (b/v). The harvesting times were 0, 2, 4, dan 6 days after elicitation. The gossypol was analyzed qualitatively and quantitatively by using High Performance Liquid Chromatography (HPLC).Gossypol content was influenced significantly by concentration of elicitor and harvesting time.The maximum ofgossypol content was obtained on the 4 day after elicitation i.e. 469.233 ± 2.332 pg/g dry weight in the elicited callus of G. hirsutum.
Pengaruh Bonggol Pisang (Musa Balbisiana Colla) sebagai MOL dan Lama Perendaman terhadap Pertumbuhan Mucuna bracteata Dini Ariyska; Bayu Pratomo; Rama R. Sitinjak; Abednego S. Karosekali; Laura J. Pinem; Edy Fachrial
Seminar Nasional Lahan Suboptimal 2020: Prosiding Seminar Nasional Lahan Suboptimal ke-8 “Komoditas Sumber Pangan untuk Meningkatkan K
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Abstract

Ariyska D, Pratomo B, Sitinjak RR, Karosekali SA, Pinem JL, & Fachrial E. 2020. The effect of banana weevil (Musa balbisiana Colla) as MOL and soaking time on the growth of mucuna bracteata. In: Herlinda S et al. (Eds.), Prosiding Seminar Nasional Lahan Suboptimal ke-8 Tahun 2020, Palembang 20 Oktober 2020. pp. xxx.  Palembang: Penerbit & Percetakan Universitas Sriwijaya (UNSRI).This study aims to determine the effect of concentration and soaking time of MOL banana weevil (Musa balbisiana Colla) on the growth of Mucuna bracteata. The banana species used in this study were bananas with seeds, and grew in abundance in several plantation areas. So that the potential is very disturbing to the main crop, namely oil palm, in other words it becomes a weed in plantations. In this study, bananas were used as a source of MOL. This study used an experimental method with a factorial randomized block design with 2 replications and 16 treatments. The first factor is the concentration of MOL banana weevil (Musa balbisiana Colla) (M) with 4 levels: M0 (0%), M1 (15%), M2 (30%), M3 (45%), the second factor is immersion time (P) with 4 levels: P0 (immersion), P1 (15 minutes), P2 (30 minutes), P3 (45 minutes). The data obtained were analyzed by means of analysis of variance with a significance of 5%. The results of data processing showed that anova did not significantly affect the interaction between the MOL concentration of banana weevil (Musa balbisiana Colla) and the immersion time on the growth of Mucuna bracteata. Various concentrations of banana weevil MOL (Musa balbisiana Colla) and soaking time did not significantly affect the growth of Mucuna bracteata plants. However, physically the growth of Mucuna bracteata is good, so that the presumption arises that this MOL actually acts as a nutrient/fertilizer for plants. Not as a growth regulator as the initial target of the research.
Pengaruh Mikroorganisme Lokal (MOL) Rebung Bambu dan Lama Perendaman terhadap Pertumbuhan Mucuna bracteata Indra Setiawan Rambe; Bayu Pratomo; Rama Riana Sitinjak; Abednego Suranta Karosekali; Laura Juita Pinem; Edy Fachrial
xxxx-xxxx
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Abstract

Rambe IS, Pratomo B, Sitinjak RR, Karosekali AS, Pinem LJ, Fachrial E. 2020. The effect of bamboo shoots as local microorganisms (MOL) and soaking time on the growth of mucuna bracteata. In: Herlinda S et al. (Eds.), Prosiding Seminar Nasional Lahan Suboptimal ke-8 Tahun 2020, Palembang 20 Oktober 2020. pp. xx.  Palembang: Penerbit & Percetakan Universitas Sriwijaya (UNSRI).The Effect of Bamboo Shoots as Local Microorganisms (MOL) and Soaking Time on The Growth of Mucuna bracteata. The research is aimed at obtaining the concentration of microorganism local bamboo shoots and long soaking the right to increase the growth of plants Mucuna bracteata. The study used an experimental method with a 2 factorial randomized block design (RBD). The first factor is the MOL concentration of the bamboo shoots which consist of 4 level that is: Control (R0), 100 ml/l (R1), 150 ml/l (R2), 200 ml/l (R3) and a factor of two is long immersion which consists of 4 level that is: Control (M0), 15 minutes (M1), 30 minutes (M2), 45 minutes (M3). The data were analyzed using (Analysis of Variance) with a significant 5% and followed by The DMRT (Duncan Multiple Range Test) with a significant 5% using SAS software version 9.3.1. Based on the results of data analysis, the results showed that the effect of local microorganism concentration (MOL) of bamboo shoots on Mucuna bracteata plants had a significant effect on tendril length at a concentration of 150 ml/l (R2) at the age of 4-5 MST and a concentration of 200 ml/l (R3) in age 6 MST, and root shoot ratio at a concentration of 150 ml/l (R2), but did not significantly affect the number of leaves, fresh and dry weight of shoots, fresh and dry weight of roots, number of root nodules, and root length. Soaking time and interaction had no significant effect on the number of leaves, tendrils length, weight of fresh and dry shoot, fresh and dry weight of root, root nodule number, root length, and the ratio of root header.