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Nico Aliannur
Laboratory of Genetics and Molecular Biology, Faculty of Mathematics and Natural Sciences, University of Lambung Mangkurat, Jl. A. Yani Km. 36, Banjarbaru, South Kalimantan
Agriculture, Biological Sciences & Forestry

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Genetic Diversity and Relationship of Durian (Durio spp.) Germplasm Based on the Internal Transcribed Spacer (ITS) Region: In Silico Analysis Dindin Hidayatul Mursyidin; Muhammad Irfan Makruf; Nico Aliannur
PLANTA TROPIKA Vol 11, No 1 (2023)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v11i1.13649

Abstract

Durian (Durio spp.) is a germplasm with a relatively high species diversity, with an estimated 27 species worldwide. However, the existence of several species has been threatened. This study aimed to reconstruct the DNA barcode of the durian and its relatives (Durio spp.) and analyze the genetic diversity and its relationship based on the internal transcribed spacer (ITS) region. Sixteen sequences of durians ITS were collected from GenBank (NCBI) and analyzed in silico using the BLAST, MultAlin, and MEGA-X software, then reconstructed phylogenetically by the UPGMA and Maximum Likelihood methods. The results show that the ITS region of Durio spp. has a base length of about 702 bp, where several mutations occur, substitution (transversion and transition) and indel (insertion and deletion). At the nucleotide level, this germplasm shows a relatively high diversity of 0.065. The cluster analyses (UPGMA and Maximum Likelihood) can separate this germplasm into four clusters and five main clades, respectively. In this study, D. zibethinus, the most popular species in the Durio genus, is closely related to D. lowianus and far from D. griffithii. This information is beneficial as reference data to support durian conservation and breeding programs, locally and globally, especially in Indonesia.
Co-Authors Muhammad Irfan Makruf Mursyidin, Dindin Hidayatul