Garuda - Garba Rujukan Digital

Genetic Diversity and Relationships of Phalaenopsis Based on the rbcL and trnL-F Markers: In Silico Approach Mursyidin, Dindin Hidayatul; Ahyar, Gusti Muhammad Zainal; Saputra, Ahmad Winarto; Hidayat, Aminoor
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.29904

In silicoÂ is the more comprehensive and applicable approach in supporting, both conservation and breeding programs of germplasm.Â The study aimed to analyze and determine the genetic diversity and relationships of 24 species ofÂ PhalaenopsisÂ using two DNA barcoding markers, namely theÂ rbcL andÂ trnL-F, by in silico approach. All sequences of theseÂ markersÂ were collected randomly from the NCBI website and analyzed using several softwares and methods, such as ClustalW and MultAlin for multiple sequence alignments and MEGA-X to determine its genetic diversity and relationships. Specifically, the genetic diversity was determined using a nucleotide diversity index and their relationships by the Maximum Likelihood method. The results showed thatÂ Phalaenopsis has a low genetic diversity of 0.24, 0.32, and 0.19, respectively. The phylogenetic analysis revealed that this orchid separated into five (for theÂ rbcL), six (trnL-F), and seven clades (a combined one), where the closest relationship is shown byÂ P. amboinensisÂ vs.Â P. venosa,Â whereas the farthest byÂ P. gibbosaÂ vs.Â P. doweryensis,Â P. stuartianaÂ vs.Â P. micholitzii, andÂ P. celebensisÂ vs.Â P. pulchra. The results have novel information on the diversity and relationships ofÂ PhalaenopsisÂ onÂ the in silicoÂ approach.Â Thus,Â our findings might be used in supporting the conservation and breeding program ofÂ Phalaenopsis, both locally and globally.

GENETIC RELATIONSHIP OF SEVERAL MORPHOLOGICAL AND MOLECULAR CHARACTERISTICS OF Phalaenopsis amabilis (L.) Blume ORCHIDS FROM THE MERATUS MOUNTAINS OF SOUTH KALIMANTAN, INDONESIA Dindin Hidayatul Mursyidin; Muhammad Rubiansyah; Badruzsaufari Badruzsaufari
Indonesian Journal of Forestry Research Vol 9, No 1 (2022): Indonesian Journal of Forestry Research
Publisher : Secretariat of Agency for Standardization of Environment and Forestry Instruments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2022.9.1.63-72

Phalaenopsis amabilis (L.) Blume orchid is one of the most popular orchid species in the world. However, this ornamental plant is threatened in its natural habitat, the Meratus Mountains of South Kalimantan, Indonesia. This study determines and analyzes the genetic relationship among several morphological characteristics of P. amabilis from this region combined with molecular (RAPD) markers. A total of ten orchid samples comprised of nine moth orchids (P. amabilis) and one species (P. cornu-cervi) as an outgroup, as well as ten RAPD primers were used in this study. Based on the morphological markers, the moth orchids have a moderate level of genetic diversity, indicated by Shannon's index value of 0.5. In contrast to molecular markers, this germplasm shows high genetic polymorphism, shown by the polymorphism degree of 100% for all primers used. The cluster analysis shows that this germplasm can be divided into two clusters for morphological and five for molecular markers. Following these markers, the grouping of moth orchids was nearly corresponding to their origin. Thus, this information could be useful as a reference for orchid conservation and breeding programs in the future.

Genetic Diversity of Elephant Foot Yam (Amorphophallus paeoniifolius) and Two Other Relatives from the Meratus Mountains of South Kalimantan, Indonesia Dindin Hidayatul Mursyidin; Muhammad Aldy Hernanda; Badruzsaufari Badruzsaufari
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 1 (2022): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.66231

Elephant foot yam (Amorphophallus paeoniifolius) is a tuber crop with high economic value, so it is very prospective to be developed. This study aimed to characterize and determine the genetic diversity and relationship of A. paeoniifolius and two other relatives from the Meratus Mountains of South Kalimantan, Indonesia, using the rbcL marker. Eight samples of A. paeoniifolius and three other ones (outgroups), two of A. muelleri and one of A. borneensis, were used in the study. The genetic diversity was determined using the nucleotide diversity index (π), whereas the phylogenetic relationships were reconstructed using the Maximum Likelihood (ML) and Neighbor-Joining (NJ) methods. The results show that this germplasm has a high diversity at an inter-species level of 0.95% and a low at intra-species (0.33%). The phylogenetic analyses revealed that Amorphophallus from this region separated into different clades, three for NJ and one for ML. In this case, A. paeoniifolius var. sylvestris from Bati-Bati, Tanah Laut is closely related to A. paeoniifolius var. hortensis from Marajai, Balangan. In conclusion, although Amorphophallus from the Meratus Mountains of South Kalimantan, Indonesia, shows a high diversity at an inter-species level, the phylogenetic analyses revealed a unique relationship. This finding is expected to be a reference in supporting efforts to conserve, cultivate, and utilize sustainable Amorphophallus, globally and locally, particularly for the Dayak Meratus community of the South Kalimantan, Indonesia.

KERAGAMAN DAN KEKERABATAN GENETIK GARCINIA BERDASARKAN KANDUNGAN SENYAWA BIOAKTIF DAN AKTIVITAS BIOLOGISNYA: KAJIAN IN SILICO Dindin Hidayatul Mursyidin; Fajar Nurrahman Maulana
BERITA BIOLOGI Vol 19, No 3A (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i3A.3862

Garcinia, belongs to the genus Guttiferae, is a very prospective plant for development. Because of apomixis, however, this plant has a narrow genetic diversity. This study aims to determine the genetic diversity and relationships of Garcinia based on the content of bioactive compounds and its biological activities, using in silico approach. A total of 64 Garcinia species were analyzed by a multivariate method using the MVSP ver. 3.1 software. The results showed that based on these characters, Garcinia had a low (narrow) genetic diversity, with a Shannon index of 0.28. However, the xanthone, antifungal activity, and leaf organ are the three of Garcinia characters with a high (wide) genetic diversity. The PCA revealed that these characters are contributed positively to this genetic diversity. The UPGMA analysis also revealed that this germplasm is divide into six main clusters, where the fifth is the largest (53 species). The farthest relationship is shown by G. hanburyi and G. cylindrocarpa, as well as G. bancana and G. excavata, at a coefficient of 0.54. In this case, G. mangostana, the most popular species of Garcinia, has the closest relationship with G. wightii at a coefficient of 0.87, and fartest to G. cylindrocarpa. This information might be valuable in supporting the preservation and breeding programs of Garcinia in Indonesia, particularly for parental selection in the development of superior cultivars.

KEANEKARAGAMAN DAN KEKERABATAN GENETIK ARTOCARPUS BERDASARKAN PENANDA DNA KLOROPLAS matK & rbcL: KAJIAN IN SILICO Dindin Hidayatul Mursyidin; Muhammad Irfan Makruf
Floribunda Vol. 6 No. 5 (2020)
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32556/floribunda.v6i5.2020.322

Keanekaragaman dan Kekerabatan Genetik Artocarpus Berdasarkan Penanda DNA Kloroplas matK & rbcL: Kajian in Silico. Floribunda 6(5): 195–206. — Artocarpus merupakan genus dari famili Moraceae yang memiliki keanekaragaman spesies tinggi. Namun akibat degradasi dan konversi habitat secara berlebihan, keberadaannya mulai terancam. Penelitian ini bertujuan untuk menganalisis keanekaragaman dan kekerabatan genetik Artocarpus secara in silico menggunakan penanda DNA kloroplas (matK dan rbcL). Sebanyak 2 set sekuen matK dan rbcL dari 48 spesies Artocarpus telah dianalisis menggunakan beberapa software, yaitu BLAST, Clustal Omega dan MEGA-X, serta direkonstruksi secara filogenetik menggunakan metode Maximum Likelihood. Hasil penelitian menunjukkan bahwa Artocarpus menunjukkan keanekaragaman genetik relatif tinggi pada tingkat nukleotida, terutama berdasarkan sekuen rbcL (0.56). Sementara itu, hasil analisis kekerabatan genetik menggunakan metode Maximum Likelihood, diperoleh gambaran bahwa Artocarpus secara umum terpisah menjadi dua (2) grup atau clade utama, baik berdasarkan sekuen matK, rbcL dan gabungan keduanya. Informasi ini diharapkan dapat dimanfaatkan untuk mendukung program pemuliaan dan pelestarian Artocarpus, terutama di Indonesia.

KANDUNGAN ASAM LEMAK OMEGA 6 PADA KETAM PASIR (Emerita spp) DI PANTAI SELATAN YOGYAKARTA Dindin Hidayatul Mursyidin
Bioscientiae Vol 4, No 2 (2007): Bioscientiae Volume 4 No 2
Publisher : Universitas Lambung Mangkurat

The objective of this study was to investigate the total content of two omega 6 fatty acids, namely linoleic acid (LA, C18:2n-6) and arachidonic acid (ARA, C20:4n-6), of mole crabs Emerita talpoida and E. Analoga. Samples of mole crabs were collected from intertidal zone of Yogyakarta’s south coast. The omega 6 fatty acids were prepared by in situ transesterification method, and were analyzed by gas chromatography. This study revealed that omega 6 fatty acid of E. talpoida consisted of 9.9% LA and 1.90% ARA, while that of E. analoga consisted of 11.11% LA and 1.83% ARA. It was concluded that the total content of omega 6 fatty acid of E. analoga was higher then that of E. talpoida.

Genetic Diversity and Relationship of Durian (Durio spp.) Germplasm Based on the Internal Transcribed Spacer (ITS) Region: In Silico Analysis Dindin Hidayatul Mursyidin; Muhammad Irfan Makruf; Nico Aliannur
PLANTA TROPIKA Vol 11, No 1 (2023)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v11i1.13649

Durian (Durio spp.) is a germplasm with a relatively high species diversity, with an estimated 27 species worldwide. However, the existence of several species has been threatened. This study aimed to reconstruct the DNA barcode of the durian and its relatives (Durio spp.) and analyze the genetic diversity and its relationship based on the internal transcribed spacer (ITS) region. Sixteen sequences of durians ITS were collected from GenBank (NCBI) and analyzed in silico using the BLAST, MultAlin, and MEGA-X software, then reconstructed phylogenetically by the UPGMA and Maximum Likelihood methods. The results show that the ITS region of Durio spp. has a base length of about 702 bp, where several mutations occur, substitution (transversion and transition) and indel (insertion and deletion). At the nucleotide level, this germplasm shows a relatively high diversity of 0.065. The cluster analyses (UPGMA and Maximum Likelihood) can separate this germplasm into four clusters and five main clades, respectively. In this study, D. zibethinus, the most popular species in the Durio genus, is closely related to D. lowianus and far from D. griffithii. This information is beneficial as reference data to support durian conservation and breeding programs, locally and globally, especially in Indonesia.

Introduksi Teknik Bioinformatika Dalam Mendukung Kegiatan Konservasi Ex-Situ Tumbuhan Langka di Kalimantan Selatan Dindin Hidayatul Mursyidin; Yudhi Ahmad Nazari; Amar Vijai Nasrulloh; Muhammad Riyan Firnanda
Jurnal Pengabdian ILUNG (Inovasi Lahan Basah Unggul) Vol 2, No 4 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/ilung.v2i4.6682

Kalimantan is part of the world's megadiversity that has high genetic diversity. However, due to habitat degradation and overexploitation, most of these biodiversity are endangered. Currently, in the Banua Botanical Garden (KRB), South Kalimantan, ex-situ conservation effort of some of rare plants has been carried out. However, this activity is still constrained by many things, one of which is the limited data collection and characterization of the collection of rare plants at the location. The objective of this study is to introduce bioinformatics techniques in supporting ex-situ conservation at the location, especially for research staff and conservation activists at the Banua Botanical Garden and several other government agencies, such as the Center for the Implementation of Environmental and Forestry Instrument Standards (BPSILHK), the Natural Resources Conservation Center (BKSDA), the Agricultural Technology Assessment Center (BPTP), the Swampland Agricultural Research Center (Balittra) and the Center for Supervision and Certification of Food Crop and Horticultural Crops Seeds (BPSBTPH), including the National Research and Innovation Agency (BRIN). In its implementation, 11 participants were present as representatives of the already mentioned institutions. In general, the activity was a success and received agood response from all participants. The evaluation showed that the output target of this activity was achieved, i.e., the increase in insight and science and technology ability of research staff and conservation activists at the Banua Botanical Garden as well as several agencies involved in the development and application of the latest bioinformatics techniques to support ex-situ conservation activities of rare plants in Kalimantan.

Introduksi Pembuatan Bioetanol Berbasis Pati dan Limbah Sagu di Kecamatan Sungai Tabuk, Banjar, Kalimantan Selatan Mursyidin, Dindin H; Susilo, Tanto Budi; Badruzsaufari, Badruzsaufari; Nazari, Yudhi Ahmad; Tuhuloula, Abubakar
Jurnal Pengabdian ILUNG (Inovasi Lahan Basah Unggul) Vol 3, No 4 (2024)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/ilung.v3i4.12289

Starch and sago waste are some of the raw materials most prospective to be developed into bioethanol. This activity aims to provide technical assistance in making bioethanol from starch and sago waste for sago farming communities in Sungai Tabuk District, Banjar, South Kalimantan. This activity is carried out in two stages, namely socialization and technical assistance in making bioethanol from starch and sago waste, as well as monitoring and evaluation. Technical socialization and assistance, including internal monitoring and evaluation, have been carried out by the implementation team for target communities. A total of 24 farmers who are members of sago cooperatives in the local area participated in socialization activities and technical assistance in making bioethanol from starch and sago waste. They were very enthusiastic to participate in the activity, as seen from the atmosphere of the discussion and the results of the questionnaire they filled out. In the next activity, several people were accompanied and intensively trained to make bioethanol using tools designed and made by the implementation team. As a result, these activities generally meet the targets and outputs of this activity, namely: (1) Increasing insight of sago farmers in Sungai Tabuk sub-district, South Kalimantan to utilize the starch and sago waste they produce into bioethanol products; (2) The establishment of a pilot project to make bioethanol from starch and sago waste on a small industrial scale managed by sago farming communities; (3) Good cooperation between the implementation team and the local sago farmers, and (4) Bioethanol products produced from starch and sago waste with unknown concentration levels.

GENETIC RELATIONSHIP OF SEVERAL MORPHOLOGICAL AND MOLECULAR CHARACTERISTICS OF Phalaenopsis amabilis (L.) Blume ORCHIDS FROM THE MERATUS MOUNTAINS OF SOUTH KALIMANTAN, INDONESIA Dindin Hidayatul Mursyidin; Muhammad Rubiansyah; Badruzsaufari Badruzsaufari
Indonesian Journal of Forestry Research Vol. 9 No. 1 (2022): Indonesian Journal of Forestry Research
Publisher : APTKLHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59465/ijfr.2022.9.1.63-72

Phalaenopsis amabilis (L.) Blume orchid is one of the most popular orchid species in the world. However, this ornamental plant is threatened in its natural habitat, the Meratus Mountains of South Kalimantan, Indonesia. This study determines and analyzes the genetic relationship among several morphological characteristics of P. amabilis from this region combined with molecular (RAPD) markers. A total of ten orchid samples comprised of nine moth orchids (P. amabilis) and one species (P. cornu-cervi) as an outgroup, as well as ten RAPD primers were used in this study. Based on the morphological markers, the moth orchids have a moderate level of genetic diversity, indicated by Shannon's index value of 0.5. In contrast to molecular markers, this germplasm shows high genetic polymorphism, shown by the polymorphism degree of 100% for all primers used. The cluster analysis shows that this germplasm can be divided into two clusters for morphological and five for molecular markers. Following these markers, the grouping of moth orchids was nearly corresponding to their origin. Thus, this information could be useful as a reference for orchid conservation and breeding programs in the future.

Title

Found 10 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 10 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 10 Documents
Search