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All Journal INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Spizaetus: Jurnal Biologi dan Pendidikan Biologi Jurnal Pengabdian Masyarakat Biologi dan Sains
Tito Nurseha
Universitas Islam Negeri Raden Fatah Palembang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Physics Social Sciences Other

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Pemberdayaan Masyarakat Kabupaten Banyuasin dalam Pemanfaatan Sekam Padi Menjadi Kertas Andi Saputra; Feliyanti Feliyanti; Riri Novita Sunarti; Ike Apriani; Ra. Hoetary Tirta Amallia; Tito Nurseha; Rr. Mini Sari Wulan; Awalul Fatiqin
Jurnal Pengabdian Masyarakat Biologi dan Sains Vol 1, No 1 (2022): Jurnal Pengabdian Masyarakat Biologi dan Sains
Publisher : Universitas Indraprasta PGRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1034.325 KB) | DOI: 10.30998/jpmbio.v1i1.950

Abstract

Paper generally uses wood as the main raw material, which is increasingly expensive, so it requires alternative raw materials such as rice husks. Banyasin Regency is a food producer and provides high by-products (rice husks), so the researchers aim to use rice husks into paper. The method used in this study includes display data (presentation of data) the results of the utilization of rice husks, and the conclusion (conclusion) of the resulting paper. The results obtained from the use of rice husk into paper in assisting the community of the Muara Telang District, Banyasin Regency, received positive responses from the community, which was indicated by the attitude of the residents in managing the husks into paper and the paper produced had characteristics, was bright brown in color, flexible, and had a husk motif. The paper obtained from this activity is suitable to be developed as a calligraphy and other craft materials.
Molecular approach to the characterization of lipase encoding genes from Moraxella sp. SBE01 Siti Soleha; Syarifah Syarifah; Tito Nurseha; Awalul Fatiqin; Endah Retnaningrum; Yitro Serang
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and Valuable Research Towards Diagnosis, Prognosis and Treatment of Dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.5596

Abstract

Lipase from Moraxella sp. SBE01 is an expression of the gene encoding lipase. Detection and characterization of the Moraxella sp. SBE01 lipase coding gene is necessary for large-scale lipase production through genetic engineering. This study aimed to observe the molecular weight, amino acid sequence, length, and conserved amino acids in the DNA encoding the lipase gene, with the goal of identifying and characterizing the lipase-coding gene from Moraxella sp. SBE01. The primer design process was conducted to amplify the lipase gene from Moraxella sp. SBE01 using specialized software for sequence alignment and phylogenetic analysis. Amplification was carried out using PCR with the designed primer, forward primer (GTC ATG ATG TAC TTC CAY GGN GGN GG), reverse primer (GGT TGC CGC CGG CDS WRT CNC C). PCR was carried out under pre-denatured conditions at 95°C (3 minutes), followed by 30 cycles of denaturation at 95°C, annealing at 66°C (30 seconds), 70°C elongations (1 minute) and final elongation of 70°C (10 minutes). The PCR results were electrophoresed using 1% agarose gel with a 1 kb DNA marker. The PCR results were sequenced and analyzed for gene and amino acid sequences and the type of lipase expressed. Sequencing resulted in 387 bp of the nucleotide sequence. The gene and amino acid sequences from Moraxella sp. SBE01 had high homology with the gene and amino acid sequences from Moraxella sp. strain TA144. The lipase gene encodes a protein consisting of 129 amino acids and contains a conserved HGG (His-Gly-Gly) motif, which is characteristic of lipases in family IV, also known as the hormone-sensitive lipase (HSL) family. This conserved sequence suggests that the lipase shares structural and functional similarities with other enzymes in the HSL family, playing a key role in lipid metabolism.
Penilaian Kualitas Air Sungai Sekanak dengan Pendekatan Mikrobiologi Fitri Hairul Nisak; Mutiara Manda Sari; Ririn Aulia RA; Ahmad Dwitanto; Tito Nurseha; Riri Novita Sunarti
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 1 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i1.439

Abstract

Sungai merupakan salah satu sumber kehidupan bagi masyarakat yang tinggal di daerah perkotaan. Banyaknya permukiman di sepanjang sungai sangat memengaruhi kualitas air dari sungai. Penurunan kualitas air ditandai dengan perubahan warna air, bau, dan adanya bakteri Coliform fecal (E. coli). Penelitian ini bertujuan untuk menganalisis kualitas air sungai Sekanak berdasarkan faktor fisika, kimia, dan mikrobiologi. Uji laboratorium yang dilakukan untuk mengetahui keberadaan bakteri Coliform yang merupakan indikator pathogen dalam air. Pengambilan sampel air menggunakan metode purposive sampling, dan uji laboratorium dengan menggunakan metode Most Probable Number (MPN). Hasil analisis mikrobiologi pada sampel air sungai sekanak 1-12 menunjukkan nilai indeks 1100/100 mL, 460/100 mL, 240/100 mL, 75/100 mL, 75/100 mL, 1100/100 mL, 64/100 mL, 1100/100 mL, 1100/100 mL, 1100/100 mL, 1100/100 mL, 2400/100 mL. 12 sampel uji menunjukkan hasil yang tidak memenuhi syarat nilai cemaran Coliform fecal (E. coli) sesuai surat keputusan menteri kesehatan Republik Indonesia Nomor 907 / MENKES / Sk / VII / 2002 menyatakan bahwa air minum harus memenuhi persyaratan dengan tingkat kontaminasi 0/100 mL untuk keberadaan Coliform. Data parameter fisika yang meliputi suhu, dan kekeruhan air, serta parameter kimia berupa pH menunjukkan hasil yang lebih besar dibandingkan baku mutu air sungai pada Peraturan Perundangan Republik Indonesia No.22 Tahun 2021 tentang penyelenggaraan perlindungan dan pengelolaan lingkungan hidup. Hal ini menunjukkan bahwa air sungai tidak memenuhi syarat untuk kebutuhan higiene sanitasi.
Co-Authors Ahmad Dwitanto Amallia, RA Hoetary Tirta Andi Saputra Endah Retnaningrum Fatiqin, Awalul Feliyanti Feliyanti Fitri Hairul Nisak Ike Apriani, Ike Mutiara Manda Sari Riri Novita Sunarti Riri Novita Sunarti Ririn Aulia RA Rr. Mini Sari Wulan Siti Soleha Syarifah Syarifah Yitro Serang