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All Journal Vegetalika Agriprima : Journal of Applied Agricultural Sciences
Fenny Irawati
University of Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Induction of Protocorm-Like Bodies (PLBs) Phalaenopsis spp. Hybrids Mutation through Ultraviolet Irradiation (UV254) and Ethyl Methane Sulfonate (EMS) Agatha Sullivania Kurniadi; Fenny Irawati; Sulistyo Emantoko Dwi Putra; Poppy Hartatie Hardjo
Agriprima : Journal of Applied Agricultural Sciences Vol 7 No 1 (2023): MARCH
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/agriprima.v7i1.512

Abstract

Phalaenopsis sp. is the most-produced orchid species in Indonesia. Compared to conventional breeding, mutation induction by using mutagens, such as Ultraviolet Light-C (λ = 254 nm) (UV254) and Ethyl Methane Sulfonate (EMS), could probably result in new superior orchid variants. This research aims to get some mutants with phenotypes that have visual differences in the Phalaenopsis spp. hybrids wild type. There were 4 durations of UV254 irradiation: 5’ on, 85’ off; 10’ on, 80’ off (1 day and 7 days for each treatment); 4 concentrations of EMS used in this research: 0.05%; 0.06%; 0.07%; 0.08% for 6 hours of immersion; selected UV254 irradiation (5’ on, 85’ off (7 days)) combined with these concentrations. UV254 irradiation treatment (5’ on, 85’ off (1 day and 7 days); 10’ on, 80’ off (7 days)) resulted in some mutants with leaf phenotypes that were visually different from the wild type; 0.05% EMS treatment resulted in PLBs mutant with a visually larger size than the wild type; 0.08% EMS treatment and combination treatments (for EMS 0.05% and 0.08% for each treatment) resulted in non-growing albino PLBs. Hence, mutation induction using UV254 and EMS in this research produced several most likely mutants having visual differences that may be more desirable than the wild type.
High Flowering Frequency of In Vitro Tomato Using 6-Benzylaminopurine (BA) and Uniform Light Wina Dian Savitri, S.Si., M.Agr.; Laurensius Dewa Senapati; Fenny Irawati; Siska Citra Dewi; Popy Hartatie Hardjo
Vegetalika Vol 15, No 3 (2026): In Publish
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.118343

Abstract

Light is essential for plant morphogenesis and flowering induction. Therefore, maintaining uniform light conditions across all cultures may enhance the success of in vitro tomato flower production. Nevertheless, limited information is available on how specific and uniform light conditions can affect in vitro flower production. Thus, the current research aimed to determine how uniform light influences in vitro flowering induction and flower production in tomato plantlets. Murashige and Skoog (MS) medium, MS medium supplemented with 1 ppm 6-Benzylaminopurine (BA), and Murashige Modified Shoot Multiplication medium were used. The effects of uniform light intensities of 1,300, 2,000, and 3,000 lux generated by white light-emitting diode grow lamps were also investigated to determine the optimum condition for flowering induction. The results showed that 1 ppm BA combined with 2,000 lux uniform light produced 100% in vitro flowers in the tomato variety Tymoti. The uniform 2,000 lux light intensity significantly improved in vitro flowering frequency. This study is the first to report on the effect of uniform light on in vitro flower induction in tomato plantlets. The results of this study will contribute to the improvement and innovations of tomato plant breeding.
Co-Authors Agatha Sullivania Kurniadi Laurensius Dewa Senapati Poppy Hartatie Hardjo Popy Hartatie Hardjo Siska Citra Dewi Sulistyo Emantoko Dwi Putra Wina Dian Savitri, S.Si., M.Agr.