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Quantity Evaluation of Laactic Acid Bacteria Using the Fncc 0027 and C410li Gene on Different Storage of Acid Bamboo Putri Widelia Welkriana; Halimatussa'diah Halimatussa'diah
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.296

Abstract

The fiber content in bamboo shoots is higher than other tropical vegetables. The water content in saw bamboo shoots is quite high, causing the bamboo shoots to have a shelf life of only 2 days. The fermentation process is characterized by the conversion of carbohydrates into lactic acid by bacteria. This study aimed to calculate LAB with FNCC 0027 and C410LI genes in tamarind shoots with different storage periods. The method used in this study is a descriptive method to describe the total plate number of microbes, LAB examination and molecular identification of bacteria found in tamarind shoots with different storage times. The results showed that all samples showed the presence of microbial TPC, examination. As for molecular identification, it was found that samples 1, 2, and 3 were identified as LAB with the C4101LI gene, while the FNCC0027 gene was not detected. Concluded that the total plate number values with different storage times of tamarind shoots, namely 0, 24, and 48 hours were 2.5x105 CFU/g, 5.4x105 CFU/g, and 7.3x105 CFU/g. In samples 1, 2, and 3, there were lactic acid bacteria of the type Bacillus plantarum C401L1.
Faktor Lingkungan Yang Berhubungan Dengan Kejadian Tuberkulosis Di Masyarakat Zuriani Rizki; Halimatussa'diah Halimatussa'diah
Barongko: Jurnal Ilmu Kesehatan Vol 4 No 2 (2026): Barongko : Jurnal Ilmu Kesehatan (Maret)
Publisher : Asosiasi Guru dan Dosen Seluruh Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59585/bajik.v4i2.1233

Abstract

ABSTRACT Tuberculosis (TB) remains a major public health problem worldwide, including in Indonesia. Environmental factors play a crucial role in the transmission of TB, particularly poor housing conditions that do not meet health standards. This study aimed to analyze the relationship between environmental factors and the incidence of tuberculosis in the community. This study used an analytic design with a cross-sectional approach. A total of 100 respondents were selected using purposive sampling technique. Data were collected through environmental observation and structured questionnaires. Data analysis was performed using univariate and bivariate analysis with chi-square test at a 95% confidence level. The results showed that there were significant relationships between ventilation (p=0.001), occupancy density (p=0.002), lighting (p=0.003), and floor condition (p=0.005) with the incidence of tuberculosis. Respondents living in houses with poor ventilation and high occupancy density had a higher risk of developing TB compared to those living in healthier environments. In conclusion, environmental factors are significantly associated with the incidence of tuberculosis. Environmental improvement and health education are strongly recommended to reduce TB cases in the community. Keywords: Tuberculosis, Environment, Ventilation, Occupancy Density ABSTRAK Tuberkulosis (TB) merupakan penyakit menular yang masih menjadi masalah kesehatan masyarakat utama di dunia, termasuk di Indonesia. Faktor lingkungan berperan penting dalam proses penularan TB, terutama kondisi rumah yang tidak memenuhi syarat kesehatan. Penelitian ini bertujuan untuk menganalisis hubungan faktor lingkungan dengan kejadian tuberkulosis di masyarakat. Penelitian ini menggunakan desain analitik dengan pendekatan cross-sectional. Sampel penelitian sebanyak 100 responden yang dipilih menggunakan teknik purposive sampling. Pengumpulan data dilakukan melalui observasi kondisi lingkungan rumah dan wawancara menggunakan kuesioner terstruktur. Analisis data dilakukan secara univariat dan bivariat menggunakan uji chi-square dengan tingkat signifikansi 95%. Hasil penelitian menunjukkan bahwa terdapat hubungan yang signifikan antara ventilasi rumah (p=0,001), kepadatan hunian (p=0,002), pencahayaan (p=0,003), dan kondisi lantai rumah (p=0,005) dengan kejadian tuberkulosis. Responden yang tinggal di lingkungan dengan ventilasi buruk dan kepadatan tinggi memiliki risiko lebih besar terkena TB dibandingkan dengan yang tinggal di lingkungan sehat. Kesimpulan penelitian ini adalah faktor lingkungan memiliki hubungan yang signifikan dengan kejadian tuberkulosis. Upaya perbaikan lingkungan dan peningkatan edukasi kesehatan sangat diperlukan untuk menurunkan angka kejadian TB di masyarakat. Kata Kunci: Tuberkulosis, Lingkungan, Ventilasi, Kepadatan Hunian