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All Journal INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY BIOEDUPAT: Pattimura Journal of Biology and Learning
Ana Bina Sari
Department Of Medical Laboratory Technology, Sekolah Tinggi Analis Bakti Asih, Bandung, Indonesia
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Social Sciences

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THE EFFECT OF TEST TUBE STERILIZATION FROM SERUM LIPEMIC AGAINST LEVELS OF TRIGLYCERIDE GPO-PAP METHOD Fitri Fadhilah; Ana Bina Sari; Astika Aprilianti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 1 (2019): The Value, Importance, and Oversight of Health Research
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i1.878

Abstract

In terms of analytic factors, it is important to define acceptable levels of common interferences, such as lipemia or hemolysis. For triglyceride, the laboratory technician must define whether samples with excess lipemia will be included in the study; this depends, in part, on whether the interferences affect the methods. In most laboratories, glass or plastic that is in direct contact associated with bio hazardous material is usually disposable. If not, it must be decontaminated according to appropriate protocols. Immediately rinsing glass or plastic supplies after use, followed by washing with a powder or liquid detergent designed for cleaning laboratory supplies and several distilled water rinses, may be insufficient. To ensure that all remaining fat from lipemic serum that attached to the tube wall has been removed, then the sterilization process is carried out so that a sterile tube is obtained. The purpose of this study is to determine the effect of test tube sterilization from serum lipemic against levels of triglyceride GPO-PAP (Glycerol-3-phosphate oxidase-p-aminophenazone) examination. This research method was a laboratory experiment. We used 8 times repetition with tubes used first are given liquid fat and cleaned by sterilization, washed with surfactant and washed with water only. By using statistical tests ANOVA obtained of this study showed results p>0.05 which is mean the treatment that used did not show a significant difference in the treatment of ordinary water-washed tubes with sterilized tubes and surfactant washed tubes. The conclusion of this study is cleaning of the test tube with the sterilization method is recommended because to avoiding the fear of remaining pollutants that can affect the results, It can also minimize the life of bacteria and viruses from the sample to be examined. However, if the sterilization method is difficult to do because of limited equipment and so on, the use of surfactants and the correct method of cleaning the tube is enough to remove impurities such as fat.
Potential of extract leaf cherry (Muntingia calabura L) on production macrophage mice (Mus musculus) Syahran Wael; Ana Bina Sari; Ine Arini; Milka Gabriel Lestuny
BIOEDUPAT: Pattimura Journal of Biology and Learning Vol 3 No 1 (2023): Bioedupat : Pattimura Journal of Biology and Learning
Publisher : Program Studi Pendidikan Biologi, Pascasarjana, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/bioedupat.v3.i1.pp95-101

Abstract

The cherry plant (Muntingia calabura L.) is a shrub or small tree only up to 12 meters high, although generally only around 3-6m. The purpose of this study was to see the application of cherry leaf extract to mice infected with Salmonella typimirium. This research is a laboratory experimental study with a post test-only control group design using Balb/c mice as the research object. This research was conducted from 10 February to 15 April 2022. The extract of cherry leaves was used at the basic biology laboratory of Pattimura University. The mice used were adult male mice aged 3 months and weighing about 20 grams. Macrophage analysis was measured from the spleen of mice using the SPPS 20 One Way Anova test and then continued with the LSD follow-up test. The results of the study showed that in the control group the average weight of the spleen was 0.126 lighter because it was not infected with Salmonella typimerium compared to the treatment of cherry leaf extract infected with Salmonella typimerium) dose of 15 mg/kgbw (0.556), dose of 75 mg/kgbw (0.516) and a dose of 150 mg/kgbw (0.510). While there was no significant difference in macrophages. Salmonella typimurium infection will initiate an inflammatory response at the site of spread of the bacteria, including reaching the spleen and causing enlargement of the spleen.
Co-Authors Astika Aprilianti Fitri Fadhilah Ine Arini Milka Gabriel Lestuny Syahran Wael